COVID-19
Our Role
Over the course of the pandemic the Infectious Diseases Laboratory has adapted and grown to support the needs our community. In 2020, our biorepository expanded to include high quality human specimens and data support for investigator research in the development of novel diagnostic and therapeutics for COVID-19.
The COVID-19 Biorepository offers prospective and retrospective specimen selection from the bank of whole blood, serum, plasma, urine, sputum, bronchioalveolar, and tissue samples from subjects hospitalized and tested positive for COVID-19.
Projects and Publications
Research Projects
| Project | Sample Quantity | Sample Type(s) |
|---|---|---|
| Surveillance with CPM (BR has only positive samples) | 893 | NP Swabs, Sputum |
| Norton HCW pilot | 85 | NP Swabs, Blood, plasma, serum, urine |
| Norton Healthcare patients (BR has only positive samples) | 406 | NP Swabs, Sputum |
| 3D swab project | 44 | NP Swabs |
| COVID-19 retrospective waste specimen | 1182 | Whole blood/ plasma/ serum, urine, NP Swabs |
| UAD 2 (Positive COVID-19) | 91 | Urine |
| Prospective sample collection project (ACRC) | 51 | Urine, Sputum, Blood/ Plasma |
| Post COVID-19 Research Clinic (CRC) | 113 | Grey top plasma, purple top blood, Tiger top serum, and gold top serum |
Sample Sharing
| Collaborator | Sample Quantity | Sample Type(s) | Date Transferred |
|---|---|---|---|
| Dr. Gabino Fernandez Botran: UofL | 30 | Plasma | August 2020 |
| Dr. Gabino Fernandez Botran: UofL | 40 | Plasma | August 2021 |
| Dr. Gabino Fernandez Botran: UofL | 40 | Plasma | September 2021 |
| Dr. Colleen Jonsson: UT HSC Regional Biocontainment Lab | 45 | NP Swabs | October 2020 |
| Dr. Colleen Jonsson: UT HSC Regional Biocontainment Lab | 45 | NP Swabs | September 2020 |
| Dr. Christina Kaufman: UofL | 21 | Whole Blood & Plasma | June 2021 |
| Dr. Haribabu Bodduluri | 60 | NP Swabs | February 2020 |
| Dr. Haribabu Bodduluri | 60 | NP Swabs | March 2021 |
| Dr. Donghoon Chung | 45 | NP Swabs | October 2020 |
Publications
February 2023
Performance of a SARS-CoV-2 RT-PCR Assay with Non-Traditional Specimen Types
Subathra Marimuthu, Holly Aliesky, Heather Ness, Daniya Sheikh, Sathya Rashmit, Dawn Balcom, Leslie A Wolf
January 2022
Impact of Pooling Samples on Analytic Sensitivity of a Real-Time Reverse Transcriptase PCR Assay for SARS CoV-2
Subathra Marimuthu, Stephen P Furmanek, Holly Aliesky, Leslie A Wolf
April 2021
Development of a real-time Reverse-Transcription PCR for SARS CoV-2 on the Luminex ARIES® Platform
Subathra Marimuthu, Holly Aliesky, Brigid Connelly, Danial Arsalan Malik, Leslie A Wolf
August 2021
Induction of interferon response by high viral loads at early stage infection may protect against severe outcomes in COVID-19 patients
Eric C. Rouchka, Julia H. Chariker, Brian Alejandro, Robert S. Adcock, Richa Singhal, Julio Ramirez, Kenneth E. Palmer, Amanda B. Lasnik, Ruth Carrico, Forest W. Arnold, Stephen Furmanek, Mei Zhang, Leslie A. Wolf, Sabine Waigel, Wolfgang Zacharias, Jose Bordon & Donghoon Chung
March 2020 - White Paper
Development and Evaluation of Two SARS-CoV-2 RT-PCR Laboratory Developed Tests on the ARIES® Automated, Sample-to-Answer, Real-Time PCR System
Arundhati Rao, DY Goldstein, Donna M. Wolk, Leslie A Wolf