Mahavir Singh, D.V.M., Ph.D.

updated 2/28/2022
Mahavir Singh, D.V.M., Ph.D.
mahavir.singh@louisville.edu
Assistant Professor (Term)


DVM: Veterinary Medicine, followed by a PhD: Cellular, and Molecular Biology

Tel. # (502) 852-3627 (Work)
Tel. # (502) 403-4125 (Cell)


 Prior Experience

  • Potentia Pharmaceuticals Inc., Louisville, KY, USA, Research & Development Division, Cell Biology, and Biotechnology Department.
  • Vaccine and Infectious Disease Organization (VIDO), Saskatoon, CANADA, Molecular Virology, and Protein-Protein Interactions during Infection, Viral Vector Development Group.

 

Dr. Mahavir Singh, and his team have been actively investigating the cellular, and molecular mechanisms of diseases employing state-of-the-art genomic, transcriptomic, metabolomic, proteomic, and epigenomic approaches. His laboratory makes use of both in vivo and in vitro disease models to understand and dissect out the complex regulatory aspects of genes’ activity, and their physiological/pathological impacts on host biology, and on how to restore health.

 

The overarching goal of Dr. Singh’s laboratory is to identify new disease pathways, and metabolic interactions that can be successfully targeted, with a translational view towards expediting the development of potential strategies to correct dysregulated cellular perturbations that are responsible for the disease etiology.

In his spare time, Dr. Singh enjoys participating in philanthropic activities.

 

Selected Publications:

  1.  Homme RP, George AK, Singh M*, Smolenkova I, Zheng Y, Pushpakumar S, Tyagi SC. Mechanism of Blood-Heart-Barrier Leakage: Implications for COVID-19 Induced Cardiovascular Injury. Int J Mol Sci. 2021 Dec 17; 22(24):13546.*Corresponding author
  2. Homme RP, Zheng Y, Smolenkova I, Singh M*, Tyagi SC. Remote Hind-Limb Ischemia Mechanism of Preserved Ejection Fraction During Heart Failure. Front Physiol. 2021 Nov 11; 12:745328.*Corresponding author
  3. Singh M*, Kapoor A, Bhatnagar A. Physiological and Pathological Roles of Aldose Reductase. Metabolites. 2021 Sep 27;11(10):655.*Corresponding author
  4. George AK, Behera J, Homme RP, Tyagi N, Tyagi SC, Singh M*. Rebuilding Microbiome for Mitigating Traumatic Brain Injury: Importance of Restructuring the Gut-Microbiome-Brain Axis. Mol Neurobiol. 2021 Aug; 58(8):3614-3627.*Corresponding author
  5. Homme RP, Sandhu HS, George AK, Tyagi SC, Singh M*. Sustained Inhibition of NF-κB Activity Mitigates Retinal Vasculopathy in Diabetes. Am J Pathol. 2021 May;191(5):947-964.*Corresponding author
  6. Tyagi SC, Singh M*. Multi-organ damage by covid-19: congestive (cardio-pulmonary) heart failure, and blood-heart barrier leakage. Mol Cell Biochem. 2021 Apr; 476(4):1891-1895.*Corresponding author
  7. Stanisic D, Jeremic N, Majumder S, Pushpakumar S, George A, Singh M*, Tyagi SC. High Fat Diet Dysbiotic Mechanism of Decreased Gingival Blood Flow. Front Physiol. 2021 Mar 3; 12:625780.*Corresponding author
  8. Singh M*, Hardin SJ, George AK, Eyob W, Stanisic D, Pushpakumar S, Tyagi SC. Epigenetics, 1-Carbon Metabolism, and Homocysteine During Dysbiosis. Front Physiol. 2021 Feb 23; 11:617953.*Corresponding author
  9. Eyob W, GeorgeAK, Homme RP, Stanisic D, Sandhu H, Tyagi SC, Singh M*. Regulation of the parental gene GRM4 by circGrm4 RNA transcript and glutamate-mediated neurovascular toxicity in eyes. Mol Cell Biochem. 2021 Feb; 476(2):663-673.*Corresponding author
  10. Tyagi SC, Stanisic D, Singh M*. Epigenetic memory: gene writer, eraser, and homocysteine. Mol Cell Biochem. 2021 Feb; 476(2):507-512. *Corresponding author
  11. George AK, Homme RP, Stanisic D, Tyagi SC, Singh M*. Protecting the aging eye with hydrogen sulfide. Can J Physiol Pharmacol. 2021 Feb; 99(2):161-170. *Corresponding author
  12. Singh M*, George AK, Eyob W, Homme RP, Stansic D, Tyagi SC. High-methionine diet in skeletal muscle remodeling: epigenetic mechanism of homocysteine-mediated growth retardation. Can J Physiol Pharmacol. 2021 Jan; 99(1):56-63.*Corresponding author
  13. Stanisic D, George AK, Smolenkova I, Singh M*, Tyagi SC. Hyperhomocysteinemia: an instigating factor for periodontal disease. Can J Physiol Pharmacol. 2021 Jan; 99(1):115-123. *Corresponding author 
  14. Stanisic D, Jovanovic M, George AK, Homme RP, Tyagi N, Singh M*, Tyagi SC. Gut microbiota and the periodontal disease: role of hyperhomocysteinemia. Can J Physiol Pharmacol. 2021 Jan; 99(1):9-17.*Corresponding author
  15. Molnar J, Mallonee CJ, Stanisic D, Homme RP, George AK, Singh M*, Tyagi SC. Hidradenitis Suppurativa and 1-Carbon Metabolism: Role of Gut Microbiome, Matrix Metalloproteinases, and Hyperhomocysteinemia. Front Immunol. 2020 Aug 19; 11:1730.*Corresponding author
  16. George AK, Singh M*, Pushpakumar S, Homme RP, Hardin SJ, Tyagi SC. Dysbiotic 1-carbon metabolism in cardiac muscle remodeling. J Cell Physiol. 2020 Mar; 235(3):2590-2598.*Corresponding author
  17. GeorgeAK, Majumder A, Ice H, Homme RP, Eyob W, Tyagi SC, Singh M*. Genes and genetics in hyperhomocysteinemia and the "1-carbon metabolism": implications for retinal structure and eye functions. Can J Physiol Pharmacol. 2020 Feb; 98(2):51-60. *Corresponding author
  18. George AK, Homme RP, Majumder A, Tyagi SC, Singh M*. Effect of MMP-9 gene knockout on retinal vascular form and function. Physiol Genomics. 2019 Dec 1;51(12):613-622.*Corresponding author
  19. Laha A, Singh M*, George, Homme RP, Tyagi SC. Dysregulation of 1-carbon metabolism and muscle atrophy: potential roles of forkhead box O proteins and PPARγ co-activator-1α. Can J Physiol Pharmacol. 2019 Nov; 97(11):1013-1017.*Corresponding author
  20. Hardin SJ, Singh M*, Eyob W, Molnar JC, Homme RP, George AK, Tyagi SC. Diet-induced chronic syndrome, metabolically transformed trimethylamine-N-oxide, and the cardiovascular functions. Rev Cardiovasc Med. 2019 Sep 30; 20(3):121-128.*Corresponding author
  21. George AK, Master K, Majumder A, Homme RP, Laha A, Sandhu HS, Suresh C Tyagi SC , Singh M*. Circular RNAs constitute an inherent gene regulatory axis in the mammalian eye and brain. Can J Physiol Pharmacol. 2019 Jun; 97(6):463-472.*Corresponding author
  22. Majumder A, Singh M*, George AK, Tyagi SC. Restoration of skeletal muscle homeostasis by hydrogen sulfide during hyperhomocysteinemia-mediated oxidative/ER-stress condition. Can J Physiol Pharmacol. 2019 Jun; 97(6):441-456.*Corresponding authorGeorge AK, Homme RP, Majumder A, Laha A, Metreveli N, Sandhu HS, Tyagi SC, Singh M*. Hydrogen sulfide intervention in cystathionine-β-synthase mutant mouse helps restore ocular homeostasis. Int J Ophthalmol.  2019 May; 12(5):754-764.*Corresponding author
  23. Singh M*, George AK, Homme RP, Majumder A, Laha A, Sandhu HS, Tyagi SC. Expression Analysis of the Circular RNA Molecules in the Human Retinal Cells Treated with Homocysteine. Curr Eye Res. 2019 Mar; 44(3):287-293.*Corresponding author
  24. Majumder A, Singh M*, George AK, Homme RP, Laha A, Tyagi SC. Remote ischemic conditioning as a cytoprotective strategy in vasculopathies during hyperhomocysteinemia: An emerging research perspective. J Cell Biochem 2019 Jan;120(1):77-92.*Corresponding author
  25. Majumder A, Singh M, Behera J, Theilen NT, George AK, Tyagi N, Metreveli N, Tyagi SC. Hydrogen sulfide alleviates hyperhomocysteinemia-mediated skeletal muscle atrophy via mitigation of oxidative and endoplasmic reticulum stress injury. Am J Physiol Cell Physiol. 2018 Nov; 315(5):C609-C622
  26. .Laha A, Majumder A, Singh M*, Tyagi SC. Connecting homocysteine and obesity through pyroptosis, gut microbiome, epigenetics, peroxisome proliferator-activator receptor γ (PPARγ) and zinc finger protein 407 (Zfp407). Can J Physiol Pharmacol. 2018 Oct; 96(10):971-976.*Corresponding author
  27. Majumder A, Singh M*, George AK, Behera J, Tyagi N, Tyagi SC. Hydrogen sulfide improves post-ischemic neoangiogenesis in the hind limb of cystathionine-β-synthase mutant mice via PPAR-γ/VEGF axis. Physiol Rep. 2018 Sep; 6(17): e13858.*Corresponding author
  28. Homme RH, Singh M*, Majumder A, George AK, Nair K, Sandhu HS, Tyagi N, Lominadze D, Tyagi SC. Remodeling of Retinal Architecture in Diabetic Retinopathy: Disruption of Ocular Physiology and Visual Functions by Inflammatory Gene Products and Pyroptosis Front Physiol. 2018 Sep 5; 9:1268. *Corresponding author
  29. Singh M*, George AK, Homme RP, Majumder A, Laha A, Sandhu HS, Tyagi SC. Circular RNAs profiling in the cystathionine-β-synthase mutant mouse reveals novel gene targets for hyperhomocysteinemia induced ocular disorders; Exp Eye Res. 2018 Sep; 174:80-92. *Corresponding author
  30. Baba SP, Zhang D, Singh M, Dassanayaka S, Xie Z, Jagatheesan G, Zhao J, Schmidtke VK, Brittian KR, Merchant ML, Conklin DJ, Jones SP, Bhatnagar A. Deficiency of Aldose Reductase Exacerbates Early Pressure Overload-Induced Cardiac Dysfunction and Autophagy in Mice. J Mol Cell Cardiol. 2018 May; 118:183-192.
  31. George AK, Singh M*, Homme RH, Majumder A, Sandhu HS, Tyagi SC. A hypothesis for treating inflammation and oxidative stress with hydrogen sulfide during age-related macular degeneration. Int J Ophthalmol. 2018 May; 11(5):881-887.*Corresponding author
  32. Singh M*, Tyagi SC. Genes and Genetics in Eye Diseases: A Genomic Medicine Approach for Investigating Hereditary and Inflammatory Ocular Disorders.  Int J Ophthalmol. 2018 Jan; 11(1):117-134.*Corresponding author
  33. Majumder A, Singh M*, Tyagi SC. Post-menopausal breast cancer: from estrogen to androgen receptor. Oncotarget. 2017 Oct; 8(60):102739-102758. *Corresponding author
  34. Singh M*, Tyagi SC. Metalloproteinases as mediators of inflammation and the eyes: molecular genetic underpinnings governing ocular pathophysiology. Int J Ophthalmol. 2017 Aug; 10(8):1308-1318. *Corresponding author
  35. Singh M*, Tyagi SC. Hyperhomocysteinemia and Age-related Macular Degeneration: Role of Inflammatory Mediators and Pyroptosis; A Proposal. Med Hypotheses. 2017 Aug; 105:17-21.*Corresponding author
  36. Singh M*, Tyagi SC. Homocysteine mediates transcriptional changes of the inflammatory pathway signature genes in human retinal pigment epithelial cells. Int J Ophthalmol. 2017 May; 10(5):696-704.*Corresponding author
  37. Singh M, Kapoor A, McCracken J, Hill B, Bhatnagar A. Aldose reductase (AKR1B) deficiency promotes phagocytosis in bone marrow derived mouse macrophages. Chem Biol Interact. 2017 Mar; 265:16-23.
  38. Singh M, Kapoor A, Bhatnagar A. Oxidative and reductive metabolism of lipid-peroxidation derived carbonyls. Chem Biol Interact. 2015 Jun; 234:261-73.
  39. Brooks AC, Guo Y, Singh M, McCracken J, Xuan YT, Srivastava S, Bolli R, Bhatnagar A. Endoplasmic Reticulum Stress-dependent Activation of ATF3 Mediates the Late Phase of Ischemic Preconditioning. J Mol Cell Cardiol. 2014 Nov; 76:138-47.
  40. Patel AK, Singh M*, Suryanarayana VV. Buffalo Alpha S1-Casein Gene 5'-Flanking Region and its Interspecies Comparison. J Appl Genet. 2014 Feb; 55(1):75-87.*Corresponding author
  41. Salabei JK, Cummins TD, Singh M, Jones SP, Bhatnagar A, Hill BG. PDGF-mediated Autophagy Regulates Vascular Smooth Muscle Cell Phenotype and Resistance to Oxidative Stress. Biochem J. 2013 May; 451(3):375-88.
  42. Moshal KS, Tipparaju SM, Vacek TP, Kumar M, Singh M, Frank IE, Patibandla PK, Tyagi N, Rai J, Metreveli N, Rodriguez WE, Tseng MT, Tyagi SC. Mitochondrial matrix metalloproteinase activation decreases myocyte contractility in hyperhomocysteinemia. Am J Physiol Heart Circ Physiol. 2008 Aug; 295(2):H890-7.
  43. Moshal KS, Zeldin DC, Sithu SD, Sen U, Tyagi N, Kumar M, Hughes WM Jr, Metreveli N, Rosenberger DS, Singh M, Vacek TP, Rodriguez WE, Ayotunde A, Tyagi SC. Cytochrome P450 (CYP) 2J2 gene transfection attenuates MMP-9 via inhibition of NF-kappabeta in hyperhomocysteinemia. J Cell Physiol. 2008 Jun; 215(3):771-81.
  44. Sen U, Moshal KS, Singh M, Tyagi N, Tyagi SC. Homocysteine-induced biochemical stress predisposes to cytoskeletal remodeling in stretched endothelial cells. Mol Cell Biochem. 2007 Aug; 302(1-2):133-43.
  45. Chauhan A, Tikoo A, Kapur AK, Singh M. The taming of the cell penetrating domain of the HIV Tat: myths and realities. J Control Release. 2007 Feb; 117(2):148-62.
  46. Moshal KS, Singh M, Sen U, Rosenberger DS, Henderson B, Tyagi N, Zhang H, Tyagi SC. Homocysteine-mediated activation, and mitochondrial translocation of calpain regulates MMP-9 in MVEC. Am J Physiol Heart Circ Physiol. 2006 Dec; 291(6):H2825-35.
  47. Sriveny D, Raina OK, Yadav SC, Chandra D, Jayraw AK, Singh M, Velusamy R, Singh BP. Cathepsin L cysteine proteinase in the diagnosis of bovine Fasciola gigantica infection. Vet Parasitol. 2006 Jan; 135(1):25-31.
  48. Singh M, Shmulevitz M, Tikoo SK. A newly identified interaction between IVa2 and pVIII proteins during porcine adenovirus type 3 infection. Virology. 2005 May 25;336(1):60-69.
  49. Singh M, Kumar V. Transgenic mouse models of hepatitis B virus-associated hepatocellular carcinoma. Rev Med Virol. Jul-Aug 2003; 13(4):243-53.
  50. Siddiqui MZ, Singh M, Gupta PK, and Das SK. Effect of feeder cells on the growth of murine hybridomas developed against recombinant bovine interferon-gamma (r-BoIFN-g). Indian J. Comp. Microbiol. Immunol. Infect. Dis. 24 (1); 2003, 71-74.
  51. Singh M*, Siddiqui MZ, and R. P. Singh. Intra-Species Mice Hybridomas Against a Recombinant Protein of Mycobacterium avium paratuberculosis. Journal of Applied Animal Research. Apr 2002; 137-144. *Corresponding author
  52. Singh M*, Bhat PP, Mishra BP, and Singh RK. Biological Transmissibility of Buffalopox Virus. Journal of Applied Animal Research. Oct 1995; 9:79-88. *Corresponding author