Mahavir Singh, D.V.M., Ph.D.

Mahavir Singh, Ph.D.
Mahavir Singh, D.V.M., Ph.D.

mahavir.singh@louisville.edu
Assistant Professor (Term)

Ph.D.: International Center for Genetic Engineering and Biotechnology (ICGEB), University of Delhi, New Delhi, INDIA, 2002
D.V.M: College of Veterinary Medicine, Ludhiana, Punjab, INDIA, 1987
Tel. # (502) 852-3627 (Work)
Tel. # (502) 403-4125 (Cell)

The Eye and Vision Science Laboratory

Research Focus

The Eye and Vision Science Laboratory in the Department of Physiology at the School of Medicine is studying the molecular mechanisms of ocular diseases employing genomics, transcriptomics, metabolomics, proteomics, and epigenomics approaches. We use both in vivo as well as in vitro models to understand and dissect-out the regulatory aspects of various genes’ activity and their impact on physiology and how to restore the homeostasis during the disease state. The overarching goal is to identify new pathways/interactions that can be successfully targeted to expedite the development of potential strategies to correct dysregulated cellular perturbations that are responsible for the disease etiology.

 
Prior Trainings/Experience

  • Senior Scientist, 2005-2010, Potentia Pharmaceuticals Inc., Louisville, KY, USA,  Research & Development Division, Cell Biology and Biotechnology Department
  • Post-doctoral Fellowship, 2001-2004, Vaccine and Infectious Disease Organization (VIDO), Saskatoon, CANADA, Molecular Virology and Protein-Protein Interactions during Infection, Viral Vector Development Group

Selected Publications:

  1. George AK, Homme RP, Majumder A , Laha A , Metreveli N , Sandhu HS, Tyagi SC , Singh M (2018). Hydrogen sulfide intervention in cystathionine-β-synthase mutant mouse helps restore ocular homeostasis, under review, International Journal of Ophthalmology.
  2. George AK, Master K,Majumder A, Homme RP, Laha A, Sandhu HS,Tyagi SC, Singh M (2018). Circular RNAs are part of an inherent gene-regulatory axis in the mammalian eye and brain. Accepted; Can J Physiol Pharmacol.
  3. Majumder A, Singh M, George AK, Tyagi SC (2018). Restoration of skeletal muscle homeostasis by hydrogen sulfide during hyperhomocysteinemia-mediated oxidative/ER-stress condition, Accepted; Can J Physiol Pharmacol.
  4. Singh M, George AK, Homme RP, Majumder A, Laha A, Sandhu HS, Tyagi SC (2018). Expression Analysis of the Circular RNA Molecules in the Human Retinal Cells Treated with Homocysteine. Curr Eye Res. Oct 27. Doi: 10.1080/02713683.2018.1542005. [Epub ahead of print].
  5. Majumder A, Singh M, George AK, Homme RP, Laha A, Tyagi SC (2018). Remote ischemic conditioning as a cytoprotective strategy in vasculopathies during hyperhomocysteinemia: An emerging research perspective. J Cell Biochem. Sep 11. Doi: 10.1002/jcb.27603. [Epub ahead of print].
  6. Homme RH, Singh M, Majumder A, George AK, Nair K, Sandhu HS, Tyagi N, Lominadze D, Tyagi SC (2018). Remodeling of Retinal Architecture in Diabetic Retinopathy: Disruption of Ocular Physiology and Visual Functions by Inflammatory Gene Products and Pyroptosis. Front Physiol. 2018 Sep 5; 9: 1268. Doi: 10.3389/fphys.2018.01268.
  7. Majumder A, Singh M, George AK, Behera J, Tyagi N, Tyagi SC (2018). Hydrogen sulfide improves post-ischemic neoangiogenesis in the hind limb of cystathionine-β-synthase mutant mice via PPAR-γ/VEGF axis. Physiol Rep.; 6(17):e13858. Doi: 10.14814/phy2.13858.
  8. Majumder A, Singh M, Behera J, Theilen NT, George AK, Tyagi N, Metreveli N, Tyagi SC (2018). Hydrogen sulfide alleviates hyperhomocysteinemia-mediated skeletal muscle atrophy via mitigation of oxidative and endoplasmic reticulum stress injury. Am J Physiol Cell Physiol. 2018 Aug 15. Doi: 10.1152/ajpcell.00147.2018. [Epub ahead of print].
  9. Singh M, George AK, Homme RP, Majumder A, Laha A, Sandhu HS, Tyagi SC (2018). Circular RNAs profiling in the cystathionine-β-synthase mutant mouse reveals novel gene targets for hyperhomocysteinemia induced ocular disorders; Exp Eye Res., 174:80-92.
  10. George AK, Singh M, Homme RH, Majumder A, Sandhu HS, Tyagi SC (2018). A hypothesis for treating inflammation and oxidative stress with hydrogen sulfide during age-related macular degeneration; Int J Ophthalmol., 11(5):881-887.
  11. Laha A, Majumder A, Singh M, Tyagi SC (2018). Connecting homocysteine and obesity through pyroptosis, gut microbiome, epigenetics, peroxisome proliferator-activator receptor γ (PPARγ) and zinc finger protein 407 (Zfp407). Can J Physiol Pharmacol.; 11: 1-6.
  12. Baba SP, Zhang D, Singh M, Dassanayaka S, Xie Z, Jagatheesan G, Zhao J, Schmidtke VK, Brittian KR, Merchant ML, Conklin DJ, Jones SP, Bhatnagar A (2018). Deficiency of Aldose Reductase Exacerbates Early Pressure Overload-Induced Cardiac Dysfunction and Autophagy in Mice. J Mol Cell Cardiol. 118:183-192.
  13. Singh M, Tyagi SC (2018). Genes and Genetics in Eye Diseases: A Genomic Medicine Approach for Investigating Hereditary and Inflammatory Ocular Disorders.  International Journal of Ophthalmology; 11(1): 117-134.
  14. Singh M, Tyagi SC (2017). Homocysteine Mediated Transcriptional Regulation in Human Retinal Pigment Epithelial Cells. International Journal of Ophthalmology; 10(5): 696-704.
  15. Majumder A, Singh M, Tyagi SC (2017). Post-menopausal Breast Cancer: From Estrogen to Androgen Receptor. Oncotarget; 8:102739-102758.
  16. Singh M, Kapoor A, McCracken J, Hill B, Bhatnagar A (2017). Aldose Reductase Deficiency Promotes Phagocytosis in Bone Marrow Derived Macrophages. Chemico-Biological Interactions; 265: 16-23.
  17. Singh M, Tyagi SC (2017). Hyperhomocysteinemia and Age-related Macular Degeneration: Role of Inflammatory Mediators and Pyroptosis; A Proposal. Medical Hypotheses; 105: 17-21.
  18. Singh M, Tyagi SC (2017). Metalloproteinases in Ocular Health and Diseases. International Journal of Ophthalmology; 10(8):1308-1318.
  19. Singh M, Kapoor A, Bhatnagar A (2015). Oxidative and Reductive Metabolism of Lipid-peroxidation Derived Carbonyls. Chem Biol Interact; 5 (234): 261-273.
  20. Babbarwal A, Patel AK, Singh M (2015). Harnessing Investigative Genomics in Heart Failure. Journal of Investigative Genomics; 2(3): 00027, http://medcraveonline.com/JIG/JIG-02-00027.pdf, an invited editorial.
  21. Patel AK, Singh M (2015). Biotechnological Approaches for Enhancing Animal Health and Production. J Dairy Vet Anim Res.; 2(1): 00023, http://medcraveonline.com/JDVAR/JDVAR-02-00023.pdf, an invited editorial.
  22. Brooks AC, Guo Y, Singh M, McCracken J, Xuan YT,Srivastava S, Bolli R, Bhatnagar A (2014). Endoplasmic Reticulum Stress-dependent Activation of ATF3 Mediates the Late Phase of Ischemic Preconditioning. J Mol Cell Cardiol. 76:138-147.
  23. Patel AK, Singh M, Suryanarayana VV (2014). Buffalo Alpha S1-Casein Gene 5'-Flanking Region and its Interspecies Comparison. J Appl Genet.; 55(1):75-87.
  24. Salabei JK, Cummins TD, Singh M, Jones SP, Bhatnagar A, Hill BG (2013). PDGF-mediated Autophagy Regulates Vascular Smooth Muscle Cell Phenotype and Resistance to Oxidative Stress. Biochem J.; 451(3):375-388.