Education and Training

Ph.D.: University of Louisville Department of Molecular, Cellular and Developmental Biology, Biology
Postdoctoral Fellowship: University of Louisville (Department of Medicine, KYINBRE Bioinformatics Core, Oral Immunology and Infectious Disease and Center for Cardiometabolic Science)

Research Interests

Dr. Singhal is an interdisciplinary medical researcher with a successful track record in integrating biological research with multi-omics technologies and data science.

She collaborates with faculty members in the department to address the center's research goal of understanding the molecular mechanisms of cardiometabolic disease, identifying predictive and prognostic biomarkers, and developing targeted novel therapeutics. Specifically, her role at the center involves discovering strategies to improve and promote cardiovascular multi-omics research by:

• Integrating wet lab experiments with advanced techniques in bioinformatics, statistical analysis, and programming to explore the mechanisms underlying cardiometabolic health and disease.
• Developing research protocols and generating customized data analysis pipelines and algorithms for processing, analyzing, and integrating multi-omics datasets that include transcriptomics, genomics, proteomics, metagenomics, metabolomics, single-cell RNA sequencing, and spatial transcriptomics.

Featured Publications

Google Scholar

• Tang XL, Nasr M, Zheng S, Zoubul T, Stephan JK, Uchida S, Singhal R, Khan A, Gumpert A, Bolli R, Wysoczynski M. Bone Marrow and Wharton's Jelly Mesenchymal Stromal Cells are Ineffective for Myocardial Repair in an Immunodeficient Rat Model of Chronic Ischemic Cardiomyopathy. Stem Cell Rev Rep. 2023 Oct;19(7):2429-2446. doi: 10.1007/s12015-023-10590-6. Epub 2023 Jul 28. PMID: 37500831; PMCID: PMC10579184.
• Kurlawala Z, McMillan JD, Singhal RA, Morehouse J, Burke DA, Sears SM, Duregon E, Beverly LJ, Siskind LJ, Friedland RP. Mutant and curli-producing E. coli enhance the disease phenotype in a hSOD1-G93A mouse model of ALS. Sci Rep. 2023 Apr 12;13(1):5945. doi: 10.1038/s41598-023-32594-5. PMID: 37045868; PMCID: PMC10097672.
• Singhal R, Lukose R, Carr G, Moktar A, Gonzales-Urday AL, Rouchka EC, Vajravelu BN. 2022. Differential Expression of Long Noncoding RNAs in Murine Myoblasts After Short Hairpin RNA-Mediated Dysferlin Silencing In Vitro: Microarray Profiling. JMIR Bioinformatics and Biotechnology, 3(1), p.e33186.
• Ghare S, Singhal R, Bryant V, Gautam S, Tirumala CC, Srisailam PK, Reyes-Vega A, Ghooray D, McClain CJ, Hoffman K, Petrosino J, Bryant K, Govind V, Cohen R, Cook RL, Barve S. Age-Associated Gut Dysbiosis, Marked by Loss of Butyrogenic Potential, Correlates With Altered Plasma Tryptophan Metabolites in Older People Living With HIV. J Acquir Immune Defic Syndr. 2022 Feb 1;89(Suppl 1):S56-S64. doi: 10.1097/QAI.0000000000002866. PMID: 35015746; PMCID: PMC8751293.
• Rouchka EC, Chariker JH, Alejandro B, Adcock RS, Singhal R, Ramirez J, Palmer KE, Lasnik AB, Carrico R, Arnold FW, Furmanek S, Zhang M, Wolf LA, Waigel S, Zacharias W, Bordon J, Chung D. Induction of interferon response by high viral loads at early stage infection may protect against severe outcomes in COVID-19 patients. Sci Rep. 2021 Aug 3;11(1):15715. doi: 10.1038/s41598-021-95197-y. PMID: 34344959; PMCID: PMC8333042.
• Singhal R, Donde H, Ghare S, Stocke K, Zhang J, Vadhanam M, Reddy S, Gobejishvili L, Chilton P, Joshi-Barve S, Feng W, McClain C, Hoffman K, Petrosino J, Vital M, Barve S. Decrease in acetyl-CoA pathway utilizing butyrate-producing bacteria is a key pathogenic feature of alcohol-induced functional gut microbial dysbiosis and development of liver disease in mice. Gut Microbes. 2021 Jan-Dec;13(1):1946367. doi: 10.1080/19490976.2021.1946367. PMID: 34369304; PMCID: PMC8354657.
• Lim GH, Singhal R, Kachroo A, Kachroo P. Fatty Acid- and Lipid-Mediated Signaling in Plant Defense. Annu Rev Phytopathol. 2017 Aug 4;55:505-536. doi: 10.1146/annurev-phyto-080516-035406. PMID: 28777926.