Pawel K. Lorkiewicz, Ph.D.

Assistant Professor of Chemistry
Technical Director, Mass Spectrometry and Bioanalytical Core,
Diabetes and Obesity Center

502-852-5750 E-mail

Education and Training

Ph.D.: University of Louisville, Analytical Chemistry
Postdoctoral Fellowship: University of Louisville, Stable Isotope Resolved Metabolomics

Research Interests

Development and implementation of workflows for Stable Isotope Resolved Metabolomics (SIRM)

SIRM technology allows for effective pathway tracing and elucidation by following the fate of isotopically labeled metabolites. The major bottleneck of this methodology is data reduction and analysis, as the mass spectral datasets consist of thousands of isotopologue peaks. Dr. Lorkiewicz's work has focused on developing appropriate frameworks to analyze, visualize and interpret SIRM data. He standardized methodology for examining glucose metabolism in cells and tissues using [13C6]-glucose and [15N2,13C]-glutamine. This data analysis methodology has been applied to studying major metabolic pathways in lung and breast cancer cells and recently in cardiac progenitor cells. Current work focuses on developing SIRM analysis frameworks to study [13C6]-glucose in-vivo, in mouse models.

Instrumental analysis and measurement of biomarkers of tobacco exposure

Dr. Lorkiewicz received funding for two pilot project human studies which measured the extent of exposure and/or levels of volatile organic compounds (VOCs) and tobacco alkaloids (TAs) in the urine and their removal over 3 hours after exposure to different tobacco products. Further, for the purpose of these AHA Tobacco Regulation and Addiction Center (ATRAC) he established robust, high-throughput liquid chromatography mass spectrometric (LC-MS) methods for detection and quantification of urinary metabolites of VOCs and TAs, as well as free biogenic monoamines and their metabolites in urine. These assays provided ATRAC with capacity to measure exposures to harmful and potentially harmful chemicals found in tobacco products and their systemic effects. Dr. Lorkiewicz's research efforts resulted in 7 publications and helped inform the design of the human tobacco study – CITU 2.0. development of a robust UHPLC-MS/MS assay - for analysis of 28 urinary metabolites of volatile organic compounds and five tobacco alkaloids. Current work focuses on developing additional LC-MS assays for measuring stress hormones and discovery of novel biomarkers of exposure to new and emerging tobacco products.

Featured Publications

Find all papers and citations via ResearchGate

  • Yeager R, Riggs DW, DeJarnett N, Srivastava S, Lorkiewicz P, Xie Z, Krivokhizhina T, Keith RJ, Srivastava S, Browning MHEM, Zafar N, Krishnasamy S, DeFilippis A, Turner J, Rai SN, Bhatnagar A. Association between residential greenness and exposure to volatile organic compounds. Sci Total Environ. 2020 Mar 10;707:135435. doi: 10.1016/j.scitotenv.2019.135435. Epub 2019 Nov 23. PubMed PMID: 31865083.
  • Keith RJ, Fetterman JL, Orimoloye OA, Dardari Z, Lorkiewicz PK, Hamburg NM, DeFilippis AP, Blaha MJ, Bhatnagar A. Characterization of Volatile Organic Compound Metabolites in Cigarette Smokers, Electronic Nicotine Device Users, Dual Users, and Nonusers of Tobacco. Nicotine Tob Res. 2020 Feb 6;22(2):264-272. doi: 10.1093/ntr/ntz021. PubMed PMID: 30759242.
  • Carll AP, Salatini R, Pirela SV, Wang Y, Xie Z, Lorkiewicz P, Naeem N, Qian Y, Castranova V, Godleski JJ, Demokritou P. Inhalation of printer-emitted particles impairs cardiac conduction, hemodynamics, and autonomic regulation and induces arrhythmia and electrical remodeling in rats. Part Fibre Toxicol. 2020 Jan 29;17(1):7. doi: 10.1186/s12989-019-0335-z. PubMed PMID: 31996220; PubMed Central PMCID: PMC6990551.
  • Conroy LR, Dougherty S, Kruer T, Metcalf S, Lorkiewicz P, He L, Yin X, Zhang X, Arumugam S, Young LEA, Sun RC, Clem BF. Loss of Rb1 Enhances Glycolytic Metabolism in Kras-Driven Lung Tumors In Vivo. Cancers (Basel). 2020 Jan 17;12(1). pii: E237. doi: 10.3390/cancers12010237. PubMed PMID: 31963621; PubMed Central PMCID: PMC7016860.
  • Nystoriak MA, Kilfoil PJ, Lorkiewicz PK, Ramesh B, Kuehl PJ, McDonald J, Bhatnagar A, Conklin DJ. Comparative effects of parent and heated cinnamaldehyde on the function of human iPSC-derived cardiac myocytes. Toxicol In Vitro. 2019 Dec;61:104648. doi: 10.1016/j.tiv.2019.104648. Epub 2019 Sep 10. PubMed PMID: 31518667.
  • Donde H, Ghare S, Joshi-Barve S, Zhang J, Vadhanam MV, Gobejishvili L, Lorkiewicz P, Srivastava S, McClain CJ, Barve S. Tributyrin Inhibits Ethanol-Induced Epigenetic Repression of CPT-1A and Attenuates Hepatic Steatosis and Injury. Cell Mol Gastroenterol Hepatol. 2019 Oct 22;9(4):569-585. doi: 10.1016/j.jcmgh.2019.10.005. [Epub ahead of print] PubMed PMID: 31654770; PubMed Central PMCID: PMC7078548.
  • Jin L, Lorkiewicz P, Malovichko MV, Bhatnagar A, Srivastava S, Conklin DJ. Acetaldehyde Induces an Endothelium-Dependent Relaxation of Superior Mesenteric Artery: Potential Role in Postprandial Hyperemia. Front Physiol. 2019 Oct 22;10:1315. doi: 10.3389/fphys.2019.01315. eCollection 2019. PubMed PMID: 31695624; PubMed Central PMCID: PMC6817488.
  • Lombardi AA, Gibb AA, Arif E, Kolmetzky DW, Tomar D, Luongo TS, Jadiya P, Murray EK, Lorkiewicz PK, Hajnóczky G, Murphy E, Arany ZP, Kelly DP, Margulies KB, Hill BG, Elrod JW. Mitochondrial calcium exchange links metabolism with the epigenome to control cellular differentiation. Nat Commun. 2019 Oct 4;10(1):4509. doi: 10.1038/s41467-019-12103-x. PubMed PMID: 31586055; PubMed Central PMCID: PMC6778142.
  • Lorkiewicz PK, Gibb AA, Rood BR, He L, Zheng Y, Clem BF, Zhang X, Hill BG. Integration of flux measurements and pharmacological controls to optimize stable isotope-resolved metabolomics workflows and interpretation. Sci Rep. 2019 Sep 23;9(1):13705. doi: 10.1038/s41598-019-50183-3. PubMed PMID: 31548575; PubMed Central PMCID: PMC6757038.
  • Lorkiewicz P, Riggs DW, Keith RJ, Conklin DJ, Xie Z, Sutaria S, Lynch B, Srivastava S, Bhatnagar A. Comparison of Urinary Biomarkers of Exposure in Humans Using Electronic Cigarettes, Combustible Cigarettes, and Smokeless Tobacco. Nicotine Tob Res. 2019 Aug 19;21(9):1228-1238. doi: 10.1093/ntr/nty089. PubMed PMID: 29868926; PubMed Central PMCID: PMC6698950.