Carolyn M. Klinge, Ph.D.

Education: Carolyn M. Klinge, Ph.D.

B.A. magna cum laude, Biology, Keuka College, Keuka Park, NY; 1979
M.S. Genetics, M.S. Hershey College of Medicine, Pennsylvania State University; 1981
Ph.D. Pharmacology, M.S. Hershey College of Medicine, Pennsylvania State University; 1984
Postdoctoral fellowship, Department of Biochemistry, University of Rochester School of Medicine, 1984-89

Curriculum Vitae

Current Positions: 

Professor of Biochemistry and Molecular Genetics, University of Louisville School of Medicine
University Scholar
Member, Experimental Therapeutics & Diagnostics Program, Brown Cancer Center

Contact Information: 

Delia Baxter Building, Room 221E
570 South Preston Street
Louisville, KY 40202
Office: 502-852-3668
Labs: 502-852-1643 and 502-852-7594
Email: carolyn.klinge@louisville.edu

Department Website:

http://louisville.edu/medicine/departments/biochemistry/our_people/klinge

Research Description:

My research centered on the molecular mechanisms of estrogen action in breast and lung cancer, endocrine resistance in breast cancer, and on sex-differences in liver responses to environmental pollutants.  I work with mice to study tissue-specific estrogen, diet, and PCB-exposure-regulated transcriptome and epitranscriptomic signaling.  I have expertise in cell-based studies on estrogen receptor-DNA interaction, transcriptional regulation of mRNA and non-coding RNAs, e.g., miRNA and lncRNAs and their gene targets, protein expression, baculovirus-production of recombinant estrogen receptors, nongenomic signaling by estrogens and endocrine disruptors, CRISPR/Cas9 knockout of gene targets, RNA- seq, miRNA-seq, m6A-RNA-immunoprecipitation seq, epitranscriptomics, and quantitative bioinformatic data analyses.  In collaboration with Dr. Matthew Cave, we are examining how epitranscriptomic changes in mouse liver following exposure to high fat or low fat diet in combination with PCB exposures regulate m6A epitranscriptome changes, splicing, transcript and protein abundance,  and pathways in non-alcoholic fatty liver disease in a mouse model of NASH.   

I teach graduate courses in the Graduate Program in Biochemistry and Molecular Genetics. I am course director for Molecular Toxicology, BMG Literature Review, and Cancer Biology. In addition I am a member of the three person biochemistry/genetics teaching team for the integrated Molecular Basis of Life, Defense, and Disease first year M.D. curriculum and provide a lecture on hormones and cancer for the second year M.D. students.  I am PI of NIH T35 DK072923 Summer Endocrine Research Training program for second year M.D. students since its initial funding in 2006.

I am PI on a DOD Breast Cancer Research Program (BCRP) grant BC220564 for a Breakthrough Award Level 2: “m6A epitranscriptome drivers of endocrine-resistant breast cancer”. This project addresses the role of m6A in the transcriptome of endocrine-sensitive versus endocrine-resistant breast cancer cells.

Examples of recent publications from my lab and of collaborations with other labs

  1. Klinge CM, Piell KM, Petri BJ, He L, Zhang X, Pan J, Rai SN, Andreeva K, Rouchka EC, Wahlang B, Beier JI, Cave MC.  Combined exposure to polychlorinated biphenyls and high-fat diet modifies the global epitranscriptomic landscape in mouse liver.  Environmental Epigenetics 2021 Sep 17;7(1):dvab008.  doi: 10.1093/eep/dvab008.  PMID: 34548932.  PMCID: PMC8448424.  (NOTE: CMK first and corresponding author)
  2. Petri BJ, Piell KM, South Whitt GC, Wilt AE, Poulton CC, Lehman NL, Clem BF, Nystoriak MA, Wysoczynski M, Klinge CM.  HNRNPA2B1 regulates tamoxifen- and fulvestrant-sensitivity and hallmarks of endocrine resistance in breast cancer cells.  Cancer Letters 2021 Oct 10;518:152-168.  doi: 10.1016/j.canlet.2021.07.015.  Epub 2021 Jul 14.  PMID: 34273466.  PMCID: PMC8358706.
  3. Metcalf S, Petri BJ, Kruer T, Green B, Dougherty S, Wittliff JL, Klinge CM, Clem BF.  Serine synthesis influences tamoxifen response in ER+ human breast carcinoma.  Endocrine Related Cancer 2021 Jan;28(1):27-37.  doi: 10.1530/ERC-19-0510.  PMID: 33112838.  PMCID: PMC7780089.
  4. Cave MC, Pinkston CM, Rai SN, Wahlang B, Pavuk M, Head KZ, Carswell GK, Nelson GM, Klinge CM, Bell DA, Birnbaum LS, Chorley BN.  Circulating microRNAs, polychlorinated biphenyls, and environmental liver disease in the Anniston Community Health Survey.  Environmental Health Perspectives 2022 Jan;130(1):17003.  doi: 10.1289/EHP9467.  Epub 2022 Jan 6.  PMID: 34989596.  PMCID: PMC8734566 .
  5. Petri BJ, Piell KM, Wahlang B, Head KZ, Andreeva K, Rouchka EC, Pan J, Rai SN, Cave MC, Klinge CM.  Multiomics analysis of the impact of polychlorinated biphenyls on environmental liver disease in a mouse model.  Environmental Toxicology & Pharmacology 2022 Aug;94:103928.  doi: 10.1016/j.etap.2022.103928.  Epub 2022 Jul 6.  PMID: 35803474.
  6. Petri BJ, Piell KM, Wahlang B, Head KZ, Andreeva K, Rouchka EC, Cave MC, Klinge CM.  Polychlorinated biphenyls alter hepatic m6A mRNA methylation in a mouse model of environmental liver disease.  Environmental Research 2023 Jan 1;216(Pt 3):114686.  doi: 10.1016/j.envres.2022.114686.  Epub 2022 Oct 28.  PMID: 36341798.
  7. Petri BJ, Klinge CM.  m6A readers, writers, erasers, and the m6A epitranscriptome in breast cancer.  Journal of Molecular Endocrinology 2022 Dec 21;70(2):e220110.  doi: 10.1530/JME-22-0110.  PMID: 36367225.  PMCID: PMC9790079.

 

Complete List of Published Work in PubMed:  http://www.ncbi.nlm.nih.gov/pubmed/?term=Klinge+CM%2C