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Jan S. Potempa

potempaName: Jan S. Potempa

Title:    Professor, PhD, DSc

Current Position:

Professor (Term) and University Scholar at UofL

Research Professor and the Departmental Head of Microbiology at the Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Krakow, Poland.

    Phone:             502 852-1319

    Fax:                 502-852-5572

    E-mail:             jspote01@louisville.edu

     

    Background

    Dr. Potempa earned his Ph.D. and D.Sc. (habilitation) in Biochemistry in 1982 and 1993, respectively, from Jagiellonian University, Krakow, Poland.  In 1985-1988 he conducted postdoctoral research at the University of Georgia and from 1989 to 2008, he worked there as a Senior Research Scientist.  From January 2009, Dr. Potempa was recruited to the University of Louisville Dental School as a Professor and Academic Scholar.  Simultaneously, Dr. Potempa maintained his teaching and research position at the Jagiellonian University since 1983, where he was head of Department of Microbiology since 2001 and promoted to Research Professor in 2005.  His current scientific interest revolves around proteolytic events in the pathogenicity of infectious diseases.  Dr. Potempa’s research achievements are highly recognized internationally as indicated by more than 6000 citations of his publications.  Of note, for a decade (1995-2005) he was the second most cited scientist in Poland.

     

    Faculty Practice Location (Address):

    University of Louisville
    School of Dentistry
    Center of Oral Health & Systemic Disease
    501 S. Preston St
    Room 256

    Louisville, KY, 40202

    Research Interests and Current Projects: (brief description):

    Dr. Potempa’s investigations are focused on proteolytic enzymes of bacterial pathogens that play important roles in the deregulation of a number of physiological pathways and evasion of host immunity.  Specifically, studies in Dr. Potempa’s laboratories concentrate on proteolytic systems of bacteria, including predominantly staphylococci and periodontal pathogens.  He discovered new families of proteinase inhibitors and proteinases, referred to as staphostatins and gingipains, respectively.  Furthermore, he found out that Porphyromonas gingivalis-derived gingipains could not only activate coagulation, fibrinolysis, and kinin pathways and signal through protease activated receptors in an unrestricted manner, but also deregulate host proteinase inhibitors through specific cleavage within their reactive site loops, thereby allowing for the additional involvement of host proteinases in the disease process.  Dr. Potempa’s current research has expanded to enzymes from Prevotella intermedia and Tannerella forsythia.  In addition to the pathophysiological significance of establishing new traits in microbial pathogenesis, these studies have led to discovery of unique proteinases in these two species of bacteria.  Dr. Potempa’s other interests are focused on bacterial pathogens interaction with the innate immunity system, a mechanistic link between periodontitis and rheumatoid arthritis, the characterization of new enzymes and elucidation of a novel protein secretion system (PerioGate) used by periodontopathogens to secrete their virulence factors.

    Teaching Activities:

    • Supervision of post-doctoral research trainees
    • Mentoring master-degree students
    • Biol 390:  Biology Honors Seminars
    • MBIO 687 Microbial Pathogenesis

    Service (current and last three years):

    • Head of Microbiology Department, Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Krakow, Poland (since 2001)
    • Committee for Dental School promotion  (Dr. David Scott)
    • Temporary Member, NIH, Dental and Craniofacial Sciences Study Section, May 27-28, 2009
    • PhD thesis supervisor (promoter) at the UofL and Jagiellonian University, Krakow, Poland
    • PhD thesis external examiner, worldwide
    • Chair of the 2008 Gordon Conference on Proteolytic Enzymes and Their Inhibitors.
    • International Scientific Advisory Panel, The 6th General Meeting of the International Proteolysis Society (IPS), Surfers Paradise QLD, Australia, October 26-30, 2009
    • ad hoc referee for Biochemica Biophysica Acta, Journal of Biological Chemistry, Infection and Immunity, European Journal of Biochemistry, Molecular Microbiology, Biochemical Journal, Journal of Bacteriology, Microbiology, Biochimie, Journal of Proteome Research, and Journal of Clinical Periodontology.

    Memberships:

    • International Proteolysis Society (IPS) (Member)
    • Polish Biochemical Society (PTBioch) (Member)
    • Graduate Faculty, Jagiellonian University, Krakow, Poland
    • The Editorial Boards member: Biological Chemistry (since 2003), Current Protein and Peptide Science (since 2004), The Open Biochemistry Journal (since 2007), Journal of Innate Immunity (since 2008), and Molecular and Oral Microbiology (previously Oral Microbiology and Immunology) (since 2010)

    Honors and Awards (Top 3-5):

    • Title of Professor, President of The Republic of Poland, Warsaw, 1998
    • Prime Minister of The Republic of Poland Award for Extraordinary Academic Achievements, Warsaw, Poland, 2001
    • The Professor’s Subsidy, Foundation for Polish Science (FNP), Warsaw, 2004
    • Award of the President of the Jagiellonian University for scientific achievements, Krakow, 2007
    • The Team Program Laureate, Foundation for Polish Science (FNP), Warsaw, 2010

    Support Staff:

    • Barbara Potempa - Research Coordinator, M.Sc., April 2009 – present
    • Zuzanna Nowakowska- Visiting Scholar, May 2013 - present

    Laboratory phone No. (502) 852-2850

     

    Publications (since 2006)

    • Journal Article (Peer review experimental research):
    1. Cronan, C.A., Potempa, J., Travis, J., & Mayo, J.A. (2006) Inhibition of gingipains by chlorhexidine: Synergistic effect of Zn(II). Oral Microbiol. Immunol. 21: 212-217.
    2. Popowicz, G., Dubin G., Stec-Niemczyk, J., Czarna, A., Dubin A., Potempa, J. & Holak, T.A. (2006) Functional and structural characterization of Spl proteases from Staphylococcus aureus. J. Mol. Biol. 358: 270-279.
    3. Białas, A., Grembecka, J., Krowarsch, D., Otlewski, J., Potempa, J., & Mucha, A. (2006) Exploring the Sn pockets in gingipains by newly developed inhibitors: Structure-based design, chemistry and activity.  J. Med. Chem. 49: 1744-1753.
    4. Matheson, N., Potempa, J., & Travis, J. (2006) Interaction of a novel form of Pseudomonas aeruginosa alkaline protease (Aeruginolysin) with interleukin-6 and interleukin-8.  Biol. Chem. 387: 911-915.
    5. Moon, J.L., Shaw, L.N., Mayo, J.A., Potempa, J., & Travis, J. (2006) Isolation and properties of extracellular proteinases of Pencillium marneffeiBiol. Chem. 387: 985-993.
    6. Smalley, J.W., Birss, J.A., Smigielski, B., & Potempa, J. (2006) The HA2 haemagglutinin domain of the lysine-specific gingipain (Kgp) of Porphyromonas gingivalis promotes µ-oxo bishaem formation from monomeric iron(III) protoporphyrin IX.  Microbiology 152: 1839-1845.
    7. Matsushita, K., Imamura, T., Tancharoen, S., Tatsuyama, S., Tomikawa, M., Travis, J., Potempa, J., Tori,i M., & Maruyama, I. (2006) Selective inhibition of Porphyromonas gingivalis growth by a factor Xa inhibitor, DX-9065a.  J. Periodontal. Res. 41: 171-176.
    8. Shaw, L.N., Aish, J., Davenport, J.E., Brown, M.C., Lithgow, J.K., Simmonite, K., Crossley, H., Travis, J., Potempa, J., Foster, S.J. (2006) Investigations into sigma B modulated regulatory pathways governing extracellular virulence determinant production in Staphylococcus aureus. J. Bacteriol188: 6070-6080.
    9. Mydel, P., Takahashi, Y., Yumoto, H., Sztukowska, M., Kubica, M., Gibson, F.C. 3rd, Kurtz, D.M., Jr., Travis, J., Collins, V.L., Nguyen, K.A., Genco, C.A. & Potempa, J. (2006) Role of the host oxidative immune response and the bacterial antioxidant, rubrerythrin, during anaerobic infection.  PLOS Pathogens 2: 712- 625.
    10. Sheets, S.M., Potempa, J, Travis, J., Fletcher, H.M., & Casiano, C.A. (2006) Gingipains from Porphyromonas gingivalis W83 synergistically disrupt endothelial cell adhesion and can induce caspase-independent apoptosis.  Infect. Immun. 74: 5667-5678.
    11. Dubin, G., Wladyka, B., Stec-Niemczyk, J., Chmiel, D., Zdzalik, M., Dubin A., & Potempa, J. (2007) The staphostatin family of cysteine protease inhibitors in Staphylococcus genus as an example of parallel evolution of protease and inhibitor specificity.  Biol. Chem. 388: 227-235.
    12. Guzik, K., Bzowska, M., Smagur, J., Krupa, O., Sieprawska, M., Travis, J., & Potempa, J. (2007) New trait in bacterial pathogenicity: Gingipains divert apoptotic cell recognition and clearance.  Cell Death Differ. 14: 171-182.
    13. Valnickova, Z., Thøgersen, I.B., Potempa, J., & Enghild, J.J. (2007) Thrombin-activable fibrinolysis inhibitor (TAFI) zymogen is an active carboxypeptidase.  J. Biol. Chem. 282: 3066-3076.
    14. Nguyn, K.A., Travis, J., & Potempa, J. (2007) Does the importance of the C-terminal residues in the maturation of RgpB from Porphyromonas gingivalis reveal a novel mechanism for protein export in a subgroup of Gram-negative bacteria?  J. Bacteriol. 189: 833-843.
    15. Kulig, P., Dubin, G., Zabel, B.A., Potempa, J., Butcher, E.C., & Cichy, J. (2007) Role of staphopain A, Staphylococcus aureus-derived cysteine protease in activation of chemerin, a new chemoattractant specific for plasmacytoid dendritic cells and macrophages.  J. Immunol. 178: 3713-3720.
    16. Vincents, B., Önnerfjord, P., Gruca, M., Potempa, J., & Abrahamson, M. (2007) Down-regulation of human extracellular cysteine protease inhibitors by the secreted staphylococcal cysteine proteases, staphopain A and B.  Biol. Chem. 388: 437-446.
    17. Popadiak, K., Potempa, J., Riesbeck, K., & Blom, A.M. (2007) Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system.  J. Immunol. 178: 7242-7250.
    18. Valnickova, Z., Thogersen, I.B., Potempa, J., & Enghild, J.J. (2007) The intrinsic enzymatic activity of procarboxypeptidase U (TAFI) does not significantly influence the fibrinolytic rate: reply to a rebuttal.  J. Thromb. Haemost. 5: 1336-1337.
    19. Kirdis, E., Jonsson, I.M., Kubica, M., Potempa, J., Josefsson, E., Masalha, M., Foster, S.J., & Tarkowski, A. (2007) Ribonucleotide reductase class III, an essential enzyme for the anaerobic growth of Staphylococcus aureus, is a virulence determinant in septic arthritis.  Microb. Pathol. 43: 179-188.
    20. Smalley, J.W., Birss, A.J., Szmigielski, B., & Potempa, J. (2007) Sequential action of R- and K-specific gingipains of Porphyromonas gingivalis in the generation of the heam-containing pigment from oxyhaemoglobin.  Arch. Biochem. Biophys. 465: 44-49.
    21. Hannes, B., Vieillard, J., Bou-Chakra, E., Mazurczyk, R., Potempa, J., Krawczyk, S., & Cabrera, M. (2008) Etching of glass patterned by microcontact printing with application to microfluidics and electrophoresis.  Sens. Actuator B-Chem. Sens. Actuator B-Chem. 129: 255-262.
    22. Josefsson, E., Kubica, M., Mydel, P., Potempa, J., & Tarkowski, A. (2008) In vivo Sortase A and Clumping factor A mRNA expression during Staphylococcus aureus infection. Microb. Pathol. 44: 103-110.
    23. Olczak, T., Sroka, A., Potempa, J. & Olczak, M. (2008) Porphyromonas gingivalis HmuY and HmuR – further characterization of a novel mechanism of heme utilization. Arch. Microbiol. 189: 197-210.
    24. Beaufort, N., Wojciechowski, P., Sommerhoff, C.P., Szmyd, G., Dubin, G., Eick, S., Kellermann, J., Schmitt, M., Potempa, J., & Magdolen, V. (2008): The human fibrinolytic system is a target for the staphylococcal metalloprotease aureolysin.  Biochem. J. 410: 157-165.
    25. Mallorquí-Fernández, N., Manandhar, S.P., Mallorquí-Fernández, G., Usón, I., Wawrzonek, K., Kantyka, T., Solà, M., Thøgersen, I.B., Enghild, J.J., Potempa, J., & Gomis-Rüth, F.X. (2008) A novel autocatalytic activation mechanism for cysteine proteases revealed by Prevotella intermedia interpain A.  J. Biol. Chem. 283: 2871-2882.
    26. Calander, A.-M., Dubin G., Potempa, J., Tarkowski, A. (2008) Staphylococcus aureus infection triggers production of neutralizing, V8-protease-specific antibodies.  FEMS Immunol. Med. Microbiol. 52: 267-272
    27. Kubica, M., Guzik, K., Koziel, J., Zarebski, M., Richter, W., Gajkowska, B., Golda, A., Maciag, A., Brix, K., Shaw, L.N., Foster, T., and Potempa, J. (2008) A potential new pathway for Staphylococcus aureus dissemination: The silent survival of S. aureus phagocytosed by human monocyte-derived macrophages.  PLoS ONE 3: e1409.
    28. Puklo, M., Guentsch, A., Hiemstra, P., Eick, S., & Potempa, J. (2008) Analysis of neutrophil-derived antimicrobial peptides in gingival crevicular fluid suggests importance of cathalicidin LL-37 in innate immune response against periodontopathogenic bacteria.  Oral Microbiol. Immunol. 23: 328-335.
    29. Uehara, A., Naito, M., Imamura, T., Potempa, J., Travis, J., Nakayama, K., & Takada, H. (2008) Dual regulation of IL-8 production in human oral epithelial cells upon stimulation with gingipains from Porphyromonas gingivalisJ. Med. Microbiol. 57: 500-507.
    30. Uehara, A., Imamura, T., Potempa, J., Travis, J., & Takada, H. (2008) Gingipains from Porphyromonas gingivalis synergistically induce the production of proinflammatory cytokines through protease-activated receptors with Toll-like receptor and NOD1/2 ligands in human monocytic cells.  Cell. Microbiol. 10: 1181-1189.
    31. Dubin, G., Stec-Niemczyk, J., Kisielewska, M., Pustelny, K., Popowicz, G., Bista, M., Kantyka, T., Boulware, K.T., Stennicke, H.R., Czarny, A., Phopaisarn, M., Daugherty, P.S., Thogersen, I.B., Enghild, J.J., Thornberry, N., Dubin, A., & Potempa, J. (2008) Enzymatic activity of the Staphylococcus aureus SplB serine protease is induced by substrates containing the sequence Trp-Glu-Leu-Gln.  J. Mol. Biol. 379: 343-356.
    32. Smalley, J.W., Birrs, A.J.. Smigielski, B., & Potempa, J. (2008) Mechanism of oxyheamoglobin breakdown by the Lysine-specific gingipain of the periodontal pathogen Porphyromonas gingivalisBiol. Chem. 389: 1235-1238.
    33. Potempa, M., Potempa,, J., Okroj, M., Popadiak, K., Eick, S., Nguyen, K.A., Riesbeck, K., & Blom, A.M. (2008) Binding of complement inhibitor C4b-binding protein contributes to serum resistance of Porphyromonas gingivalis.  J. Immunol. 181: 5537-5544.
    34. Shaw, L.N., Lindholm, C., Prajsner, T., Miller, K.K., Brown, M.C., Golonka, E., Stewart, G.C., Tarkowski, A., & Potempa, J. (2008) Identification and characterization of Sigma S, a novel missing component of the stress and virulence responses of Staphylococcus aureusPLoS ONE 3: e3844.
    35. McAlister, A.D., Sroka, A., Fitzpatrick, R., Quinsey, N.S., Travis, J., Potempa, J., & Pike, R.N. (2008) Gingipain enzymes from Porphyromonas gingivalis preferentially bind immobilised extracellular proteins: a mechanism favouring colonisation?  J. Periodontal. Res. 44: 348-353.
    36. Guentsch, A., Puklo, M., Preshaw, P.M., Glockmann, E., Pfister, W., Potempa, J., & Eick, S. (2009) Neutrophils in chronic and aggressive periodontitis in interaction with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. J. Periodontal. Res. 44: 368-377.
    37. Fitzpatrick, R.E., Campbell, P., Sivagurunathan, S., Pagel, C.N., Potempa, J., Mackie, E.J., & Pike, R.N. (2008) The gingipains from Porphyromonas gingivalis do not directly induce osteoclast differentiation.  J. Periodontal. Res. 44: 565-567.
    38. Smagur, J., Guzik, K., Magiera, L., Bzowska, M., Gruca, M., Thøgersen, I., Enghild, J.J., & Potempa, J. (2009) A new pathway of staphylococcal pathogenesis: Apoptosis-like death induced by Staphopain B (SspB) in human peripheral blood neutrophils and monocytes.  J. Innate Immun. 1: 98-108
    39. Fitzpatrick, R.E., Aprico, A., Lakshmi C. Wijeyewickrema, L.C., Pagel, C.N., Wong, D., Potempa, J., Eleanor J., E.J., & Pike, R.N. (2009) High molecular weight gingipains from Porphyromonas gingivalis induce cytokine responses from macrophage-like human cells via a non-proteolytic mechanism.  J. Innate Immun. 1: 109-117.
    40. Nguyen, K.A., Żylicz, J., Szczesny, P., Sroka, A., Hunter, N., & Potempa, J. (2009) Deciphering a novel protein secretion system in Porphyromonas gingivalis: PorT as an outer membrane protein.  Microbiology 155: 328-337.
    41. Yasuhara, R., Miyamoto, Y., Takami, M., Imamura, T., Potempa, J., Yoshimura, K., & Kamijo, R. (2009) Lysine-specific gingipain promotes lipopolysaccharide- and active vitamin D3-induced osteoclast differentiation by degrading osteoprotegerin.  Biochem. J. 419: 159-166
    42. Stec-Niemczyk, J., Pustelny, K., Kisielewska, M., Bista, M., Boulware, K.T., Stennicke, H.R., Thogersen, I.B., Daugherty, P.S., Enghild, J.J., Dubin, A., Potempa, J. & Dubin, G. (2009) Structural and functional characterization of SplA, an exclusively specific protease of Staphylococcus aureus. Biochem. J. 419: 555-564.
    43. Smagur, J., Guzik, K., Bzowska, M., Kuzak, M., Zarebski, M., Kantyka, T., Walski, M., Gajkowska, G. & Potempa, J. (2009) Staphylococcal cysteine protease staphopain B (SspB) induced rapid engulfment of human neutrophils and monocytes by macrophages.  Biol. Chem. 390: 361-371.
    44. Potempa, M., Potempa, J., Kantyka, T., Nguyen, K.A., Wawrzonek, K., Manandhar, S.P., Popadiak, K., Riesbeck, K., Eick, S. and Blom A.M. (2009) Interpain A, cysteine proteinase from Prevotella intermedia inhibits complement by degrading complement factor C3.  PLoS Pathogens 5: e1000316
    45. Chakra, E.B., Hannes, B., Vieillard, J., Mansfield, C.D., Mazurczyk, R., Bouchard, A., Potempa, J., Krawczyk, S., & Cabrera, M. (2009) Grafting of antibodies inside integrated microfluidic-microoptic devices by means of automated microcontact printing. Sens. Actuator B-Chem. Sens. Actuator B-Chem. 140: 278-286.
    46. Koziel, J., Maciag-Gudowska, A., Mikolajczyk, T., Bzowska, M., Adeline R. Whitney, A.R., Shaw, L.N., DeLeo F.R., & Potempa, J. (2009) Phagocytosis of Staphylococcus aureus by macrophages exerts cytoprotective effects manifested by the upregulation of antiapoptotic factors.  PLoS ONE 4: e5210.
    47. Wojtowicz, H., Guevara, T., Tallant, C., Olczak, M., Sroka, A., Potempa, J., Solà, M., Olczak, T., & Gomis-Rüth, F.X. (2009) Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis. PLoS Pathogens 5: e1000419
    48. Stathopoulou, P.G., Galicia, J.C., Benakanakere, M.R., Garcia, C.A., Potempa, J., & Kinane, D.F. (2009) Porphyromonas gingivalis induce apoptosis in human gingival epithelial cells through a gingipain-dependent mechanism. BMC Microbiol. 9: 107.
    49. Monteiro, A.C., Scovino, A., Raposo, S., Gaze, V.M., Cruz, C., Svensjo, E., Narciso, M.S., Colombo, A.C., Pesquero, J.B., Feres-Filho, E., Nguyen, K.-A., Sroka, A., Potempa, J., & Scharfstein, J. (2009) Induction of fimbriae-specific IFN-g and IL-17-producing T cells in mice orally infected by Porphyromonas gingivalis depends on bradykinin B2 receptor activation by kinin danger signals proteolytically generated by gingipain. J. Immunol. 183: 3700-3711.
    50. Kantyka, T., Latendorf, T., Wiedow, O, Dubin, G., Bartels, J., Gläser, R., Schröder, J.-M., Potempa, J., & Meyer-Hoffert, U. (2009) RgpB from Porphyromonas gingivalis specifically inactivates the elastase inhibitor Elafin by distinct proteolytic cleavage.  Biol. Chem. 390: 1313-1320.
    51. Byrne, D., Wawrzonek, K., Jaworska, A., Birss, A.J., Potempa, J., & Smalley, J.W. (2010) Role of the cysteine protease Interpain A of Prevotella intermedia in breakdown and release of haem from haemoglobin. Biochem. J. 425: 257-264.
    52. Karim, A.Y., Kulczycka, M., Kantyka, T., Dubin, G., Jabaiah, A., Daugherty, P.S., Thogersen I.B., Enghild, J.J., Ky-Anh Nguyen, K.-A., and Potempa, J. (2010) A Novel Matrix Metalloprotease-like Enzyme (Karilysin) of the Periodontal Pathogen Tannerella forsythia ATCC 43037.  Biol. Chem. 391: 105-117.
    53. Koziel, J., Karim, A.Y., Przybyszewska, K., Ksiazek, M., Rapala-Kozik, M., Nguyen, K.-A., & Potempa, J. (2010) Proteolytic inactivation of LL-37 by karilysin, a novel virulence mechanism of Tannerella forsythia. J. Innate Immun. 2: 288-293.
    54. Wegner, N., Sroka, A., Eick, S., Wait, R., Nguyen, K.-A., Lundberg, K., Culshaw, S., Potempa, J., & Venable, P.J. (2010) Peptidylarginine deiminase from Porphyromonas gingivalis citrullinates human fibrinogen: Implications for autoimmunity in rheumatoid arthritis.  Arthrits Rheum. 62: 2662-2672
    55. Ishihara, K., Wawrzonek, K., Shaw, R.N., Inagaki, S., Okuda, K., Potempa, J. (2010) Dentipain, a Streptococcus-pyogenes-IdeS-protease homologue, is a novel virulence factor of Treponema denticola.  Biol. Chem. 391: 1047-1055.
    56. Zdzalik, M., Pustelny, K., Kedracka-Krok, S., Huben, K., Pecak, A., Wladyka, B., Jankowski, S., Dubin, A., Potempa, J. & Dubin, A. (2010) Interaction of regulators Mdm2 and Mdm4 with transcription factors p53, p63, and p73.  Cell Cycle 9: 4575-4582.
    57. Cerdà-Costa, N., Karim, A.Y., Guevara, T., Ksiazek, M., Nguyen, K.A., Potempa, J. & Gomis-Rüth, F.X. (2011) The structure of Tannerella forsythia karilysin catalytic domain reveals it is a bacterial xenolog of animal matrix metalloproteinases. Mol. Microbiol. 79: 119-132.
    58. Rapala-Kozik, M., Cholewa, G., Chruscicka, B., Karkowska-Kuleta, J., Sroka, A., Harwald, H., Nguyen, K.A., Eick, S., Potempa, J., & Kozik, A.: Adsorption of components of the plasma kinin-forming system on the surface of Porphyromonas gingivalis involves gingipains as the major docking platforms. Infect. Immun. 79: 797-805.
    59. Smalley, J.W., Byrne, D.P., Birss, A.J., Wojtowicz H., Sroka A., Potempa, J., & Olczak, T. (2011) HmuY haemophore and gingipain proteases constitute a unique syntrophic system of heam acquisition by Porphyromonas gingivalis.  PLoS ONE 6: 217182.
    60. Ohbayashi, T., Irie, A., Murakami, Y., Nowak, M., Potempa, J., Nishimura, Y., Shinohara, M. & Imamura, T. (2011) Degradation of fibrinogen and collagen by staphopains, cysteine proteinases released from Staphylococcus aureus. Microbiology 157: 786-792.
      1. Guentsch, A., Kramesberger, M., Sroka, A., Glockmann, E., Pfister, W., Potempa, J., Jentsch, H., & Eick, S. (2011) Comparison of gingival crevicular sampling methods in patients with severe chronic periodontitis.  J. Periodontol. (in press), PMID: 21235330
      2. Kantyka, T., Plaza, K., Koziel, J., Florczyk, D., Stennicke, H.R., Thogersen, I.B., Enghild, J.J., Silverman, G.A., Pak, S.C., & Potempa, J. (2011) Inhibition of Staphylococcus aureus cysteine proteases by human serpin potentially limits staphylococcal virulence. Biol. Chem. 392: 493-489.
      3. Golda, A., Malek, N., Dudek, B., Zeglen, S., Wojarski, J., Ochman, M., Kucewicz, E., Zembala, M., Potempa, J., & Pyrc, K.: Infection with human coronavirus NL63 enhances streptococcal adherence to epithelial cells.  J. Gen. Virol. (in press)
      4. Guzik, K., Skret, J., Smagur, J., Bzowska, M., Gajkowska, B., Scott, D. & Potempa, J. (2011) Ciggarett smoke-exposed neutrophils die unconventionally but are rapidly phagocytosed by macrophages. Cell Death Disease 2: e131)

    • Review Articles:
    1. Golonka, E., Travis, J., Potempa, J., & Shaw, L.N. (2006) Poison-antidote systems in bacteria: the co-evolution of functional counterparts.  Cell. Mol. Biol. 52: 18-22
    2. Guzik, K., & Potempa, J. (2008) Friendly fire against neutrophils: proteolytic enzymes confuse the recognition of apoptotic cells by macrophages. Biochimie 90: 405-415.
    3. Koziel, J., Maciag-Gudowska, A., & Potempa, J. (2008) Staphylococcus aureus as an intracellular pathogen of immunological cellsPostepy Microbiol. 47: 393-399 (in Polish).
    4. Potempa, J., & Pike, R.N. (2009) Corruption of innate immunity by bacterial proteases.  J. Innate Immun. 1: 70-87
    5. Krauss, J.L., Potempa, J., Lambris, J.D., & Hajishengallis, G. (2010) Complementary Tolls in the periodontium: How periodontal bacteria modify complement and Toll-like receptor responses to prevail in the host.  Periodontology 2000 52: 141-162.
    6. Mangat, P., Wegner, N., Venables, P.J., & Potempa, J. (2010) Bacterial and human peptidylarginine deiminases: targets for inhibiting the autoimmune response in rheumatoid arthritis?  Arthritis Res. Ther. 12: 209
    7. Guo, Y., Ky-Anh Nguyen, K.A. & Potempa, J. (2010) Dichotomy of gingipains action as virulence factors: from cleaving substrates with the precision of a surgeon’s knife to a meat chopper-like brutal degradation of proteins.  Periodontology 2000, 54: 15-44.
    8. Kantyka, T., Rawlings, N.D., & Potempa, J. (2010) Prokaryote-derived protein inhibitors of proteases: a sketchy occurrence and mostly unknown function.  Biochimie 92: 1644-1650..

     

    • Book Chapters:
    1. Potempa, J., Kantyka, T., & Nelson, D. (2007) Serpins interaction with bacterial proteases. In: Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity (G. A. Silverman and D. A. Lomas, Eds.), pp. 425-444, New Jersey, World Scientific Publishing Company
    2. Potempa, J., & Nguyen, K.A. (2007) Purification and characterization of gingipains.  Curr. Protoc. Protein Sci. (J.E. Coligan, B.M. Dunn, D.W. Speicher, and P.T. Wingfield, eds.), John Wiley and Sons, Inc., Malden, MA, USA, Suppl. 49: 21.19.1-21.19.16
    3. Kantyka, T., Shaw, L.N. & Potempa, J. (2011) Papain-like proteases of Staphylococcus aureus. In: Cathepsin Proteases of Pathogenic Organisms (Eds. Mark W. Robinson & John P. Dalton), Springer and Landes Bioscience, pp. 1-14.
    4. Yongqing, T., Potempa, J., Pike, R.N. and Wijeyewickrema, L.C. (2011) The lysine-specific gingipain of Porphyromonas gingivalis: importance to pathogenicity and potential strategies for inhibition.  In: Cathepsin Proteases of Pathogenic Organisms (Eds. Mark W. Robinson & John P. Dalton), Springer and Landes Bioscience, pp. 15-29.
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