Research Personnel Qualifications and Training

One of the primary responsibilities of the IACUC is to ensure that research personnel have the qualifications and training necessary to perform proposed work using animal subjects. 

Level I - Does not allow unescorted access into animal facilities; for individuals exposed to research facilities/lab animals on single occasions with no intention of working independently.

Level II - Required for all personnel wishing to work with laboratory animals and for access into animal facilities.

Level III- "hands-on" one-on-one or group sessions for practical training with animals; may be tailored to the needs of the individual or laboratory.



Periodically, individuals must be exposed to laboratory animals or research facilities on single occasions with no intention of working independently (e.g., students involved in a teaching exercise involving animals, project sponsors wishing to review or participate in a procedure). Although these individuals must understand their responsibilities and limitations, as well as the hazards associated with research animal contact, the depth of training necessary is minimal. “Level I Training” consists of the informative brochures entitled, “The Use of Animals in Biomedical Research at the University of Louisville,” and “Introduction to Occupational Hazards Associated with the Care and Use of Laboratory Animals.”

These visiting personnel must remain under the supervision of a Level II trained staff member.

To ensure a basic level of understanding the animal welfare responsibilities and occupational health risks associated with animal use, documented receipt and review of Level I Training materials is also required for individuals prior to IACUC approval as project participants until Level II Training is completed. This includes the requirement for supervision by a Level II trained staff member during all animal use.




The IACUC also sponsors monthly didactic (Level II) and "hands-on" (Level III) training sessions for scientists, research technicians, and others involved in animal research. Level II is a training program, which is described in the IACUC Policy entitled "Required Training" is designed to ensure that these individuals understand the principles of humane animal care and are qualified to handle the species proposed for use. All research personnel listed on an animal-use proposal must attend a "Level II" seminar within 90 days of their association with an animal research study. Those who have not met training requirements at the time of proposal submission are informed of the need to do so during the proposal review process; animal use privileges are contingent upon meeting the IACUC training requirements.


2018 Level II Training Schedule


Contents of Level II Packet:

Instructional Material & Info

Use of Animals in Biomedical Research

Occupational Health ans Safety Enrollment in CITI

Periodic Health Survey

Information Sheet: The Use of SASPs

List of Approved IACUC Policies

Policies and Procedure: Reporting and Investigating animal Welfare Concerns

IACUC Member Roster and Key Personnel Contact



The Research Resources Facility (RRF) has been offering Level III training for Principal Investigators and laboratory personnel on an individual basis. Generalized Level III training consists of standard techniques: appropriate animal handling and restraint, physical exam, blood collection (intravascular, vessel stick, terminal cardiac puncture), injection techniques (subcutaneous, intravascular, intraperitoneal, intramuscular), and euthanasia.

Level III training is intended to help new or established personnel at any level develop and/or hone techniques common to most all research efforts.  The RRF offers Level III training with requested species as necessary, .

Unless otherwise specified, Level III Training will be held in the RRC which is located in the basement of the Medical Dental Research (MDR) building (at Floyd St. and Abraham Flexner). Click here for MAP to MDR facility.

When entering the main entrance of the MDR (Floyd street side), take the elevators to the basement. You will see a vending machine area where you can wait once you exit the elevators; a technicians will be around to gather everyone and direct groups to the main training room.