Research Personnel Qualifications and Training

One of the primary responsibilities of the IACUC is to ensure that research personnel have the qualifications and training necessary to perform proposed work using animal subjects. Below are brief summaries of the training courses offered, please scroll down for more information and links. 

Level I - Does not allow unescorted access into animal facilities; for individuals exposed to research facilities/lab animals on single occasions with no intention of working independently.

Level II "Working with the IACUC" - Required for all personnel wishing to work with laboratory animals and for access into animal facilities. *Level II Training is now offered online. Read below for more information.*

Level III - "hands-on" one-on-one or group sessions for practical training with animals; may be tailored to the needs of the individual or laboratory.

Euthanasia Training (effective February 1, 2019) - Required for all laboratory personnel responsible for euthanasia. All new Proposals, 3-year renewal Proposals, and study participant additions will be subject to this euthanasia training requirement. *Read below for more information*


Periodically, individuals must be exposed to laboratory animals or research facilities on single occasions with no intention of working independently (e.g., students involved in a teaching exercise involving animals, project sponsors wishing to review or participate in a procedure). Although these individuals must understand their responsibilities and limitations, as well as the hazards associated with research animal contact, the depth of training necessary is minimal. “Level I Training” consists of the informative brochures entitled, “The Use of Animals in Biomedical Research at the University of Louisville,” and “Introduction to Occupational Hazards Associated with the Care and Use of Laboratory Animals.”

These visiting personnel must remain under the supervision of a Level II trained staff member.

To ensure a basic level of understanding the animal welfare responsibilities and occupational health risks associated with animal use, documented receipt and review of Level I Training materials is also required for individuals prior to IACUC approval as project participants until Level II Training is completed. This includes the requirement for supervision by a Level II trained staff member during all animal use.




Level II Training ("Working with the IACUC" in CITI) is required for all personnel wishing to work with laboratory animals and for access into animal facilities.Level II is a training program that is designed to ensure that these individuals understand the principles of humane animal care and are qualified to handle the species proposed for use. The IACUC Policy entitled "Required Training" describes Level II Training in detail, along with the other offered training programs. All research personnel listed on an animal-use proposal must complete the "Level II" course before they can participate on an animal research study. In addition, Level II refresher training is required on a triennial basis to ensure that all personnel working with animals stay informed of the regulatory requirements. Those who have not met training requirements at the time of proposal submission are informed of the need to do so during the proposal review process; animal use privileges are contingent upon meeting the IACUC training requirements.

Level II Training is now offered online through CITI Program as a set of courses titled “Working with the IACUC.”  Currently, no in-person training sessions are planned and all Level II Training should be completed online, unless a special request is made through the IACUC office for an in-person session.

Link to CITI Program

Instructions on Registering for Level II ("Working with the IACUC"):

Registering a NEW CITI Account for Level II (for users who do not already have a CITI account)

Registering an EXISTING CITI Account for Level II (for users who have a CITI account)


You will receive additional instructional materials during your facility orientation:

Instructional Materials & Info

Information Sheet: The Use of SASPs

List of Approved IACUC Policies

Policies and Procedure: Reporting and Investigating Animal Welfare Concerns

Caution: Laboratory Animal Allergies Sign

IACUC Member Roster and Key Personnel Contact



The Research Resources Facility (RRF) offers Level III training for Principal Investigators and laboratory personnel regularly, as well as on an individual basis. Generalized Level III training consists of standard techniques: appropriate animal handling and restraint, physical exam, blood collection (intravascular, vessel stick, terminal cardiac puncture), injection techniques (subcutaneous, intravascular, intraperitoneal, intramuscular), and euthanasia.

Level III training is intended to help new or established personnel at any level develop and/or hone techniques common to most all research efforts. The RRF has posted its Level III Training schedule for 2019, which you may register for through the IACUC website. The RRF provides Level III training for Principal Investigators and laboratory personnel once a month with mice. The training is scheduled for the third Wednesday of each month at 1PM and lasts approximately 2 hours.

The RRF also offers Level III training with specialized techniques or other species as necessary, .

Euthanasia Training

Effective February 1, 2019: All laboratory personnel responsible for euthanasia must attend a hands-on euthanasia training session with RRF faculty and/or staff. The IACUC is also offering the opportunity for key laboratory personnel to become RRF-certified euthanasia trainers to train individuals within their lab; lab personnel wanting to become RRF-certified euthanasia trainers must attend a hands-on euthanasia trainer certification course with RRF vet staff. The RRF offers training monthly, as well as on an individualized basis, for both the euthanasia course and the euthanasia trainer certification course. All new Proposals, 3-year renewal Proposals, and study participant additions will be subject to this euthanasia training requirement. Further information is available in the IACUC policy, "Required Training." Information regarding available training sessions is available through the "Euthanasia Training Registration" tab. 

RRF-certified euthanasia trainers should document any training sessions they conduct with the Euthanasia Training Sign-In Sheet and promptly email the IACUC Office after the sessions with sign-in sheet of participants and species-specific methods of euthanasia taught.