Animal Users & Facility Access

Below is a summary of the 5 requirements that all personnel wishing to work with laboratory animals and/or gain facility access must complete. Note: Principal Investigators on an animal use proposal must complete these requirements regardless of whether they will be directly working animals.

Once you have completed all of the steps below, you will have fulfilled the IACUC training requirements and can be added to an IACUC Proposal in iRIS. Your Principal Investigator or Laboratory Manager will submit a request to have you added to an IACUC Proposal, and you will receive an email from iRIS once you are approved. After you are approved as a project participant on a proposal in iRIS, you can submit a facility access request.

Educational information about each training requirement is available here.

1. Complete the following 2 courses in CITI Program:

  • Working with the IACUC (also known as "Level II Training")
  • Occupational Health and Safety Training for Animal Handlers

Instructions for Registering a NEW CITI Account for Level II and OHS(for users who do not have a CITI account) NOTE: You must register with the UofL ID version of your email address (e.g.,

Instructions for Adding new courses to an EXISTING CITI Account for Level II and OHS(for users who already have a CITI account)

Link to CITI Program


2. Complete the Periodic Animal Health Survey.

Health surveys should be submitted directly to Campus Health via email to  with the subject line "Animal Contact Health Survey"

NOTE:  Health Surveys may take 1-2 weeks to process due to COVID responsibilities of Campus Health as well as the student return to campus.

3. Fill out your IACUC Participant Training Log.

You should record all prior experience and training. All training and experience should be species-specific. Note: you should periodically update your training log to reflect all newly-obtained training. Training should be obtained and documented prior to performing any animal-related procedures unsupervised. An Information Sheet for Training Log Completion is available here. Note: Users can now assign editors to their training log. Instructions on how to assign editing or viewing privileges are available HERE. Go to theTraining Log Website

4. Complete Euthanasia Training, if applicable.

If you are responsible for euthanasia, you must attend a hands-on euthanasia training session with CMRU veterinary faculty/staff or an CMRU-certified euthanasia trainer. Register to take a euthanasia training course with the CMRU here.

5. Complete Rodent Surgery Training, if applicable.

If you are responsible for performing surgery (both survival and non-survival) on rodents, you must complete IACUC-mandated training requirements:

  • CITI Program Course: “Post-Procedure Care of Mice and Rats in Research: Minimizing Pain and Distress”
  • CITI Program Course: “Rodent Aseptic Surgery" (see instructions in #1 above to register for a CITI Account and add courses)
  • Rodent Surgery I and II, or Surgery Observation [Register for courses here]

Link to CITI Program

Educational information about each component of IACUC Training

Note: See the instructions above for materials/links to complete these requirements

All personnel wishing to work with laboratory animals and requesting access into animal facilities must complete this training before they will be approved as project participants and granted facility access. Level II Training is now offered online through CITI Program as a set of courses titled “Working with the IACUC.”Currently, no in-person training sessions are planned and all Level II Training should be completed online, unless a special request is made through the IACUC office for an in-person session.

Level II is designed to ensure that individuals understand the principles of humane animal care and are qualified to handle the species proposed for use. All research personnel listed on an animal-use proposal must complete the "Level II" course before they can participate on an animal research study. In addition, Level II refresher training is required every three years to ensure that all personnel working with animals stay informed of the regulatory requirements. Those who have not met training requirements at the time of proposal submission are informed of the need to do so during the proposal review process; animal use privileges are contingent upon meeting the IACUC training requirements.

See the IACUC policy "Required Training" for further information about Level II "Working with the IACUC".

Enrollment in the Occupational Health and Safety Program (OHSP) is mandatory for all personnel exposed to animals or animal areas and must be renewed every three years.OHSP enrollment consists of 2 parts: a CITI training course and the Individual Risk Assessment (the Periodic Animal Contact Health Survey). The CITI training course, titled "Occupational Health and Safety Training for Animal Handlers," includes information about animal-related hazards such as allergies, bites, and scratches; zoonotic diseases; physical hazards such as "sharps," electrical hazards, and compressed gas cylinders; and the use of hazardous agents. All individuals wishing to work independently with animals must take this course and successfully pass a quiz based on its contents.

The University has a multidisciplinary approach to assessing hazards associated with the use of animals and potentially hazardous agents. Animal facilities are designed with engineering controls over potential hazards; operational practices and personal protective equipment (PPE) augment this control. For example, research personnel working in animal facilities are required to wear a clean laboratory coat or disposable gown as minimal protection. Disposable gloves are available in all animal-housing rooms; their use is strongly encouraged during any animal handling activities. Depending on the health status of the animals involved or the use of hazardous agents in research, additional PPE may be required, such as additional disposable gowns, masks, shoe covers, and special gloves (e.g., nitrile). Project-specific PPE requirements are outlined in individual Special Animal Safety Protocols (SASPs).

Your understanding of potential hazards in animals and animal facilities does not prepare you to mitigate risks associated with your personal medical status. Conditions such as pregnancy, immune-suppression, and preexisting allergies may increase risks of occupationally-acquired illness. The Individual Risk Assessment allows individualized risk assessment by an OHS Physician. You are encouraged to complete a new Periodic Animal Contact Health Survey if your health or medical status changes in any way. You will be required to submit a new survey at least once every three years.

NOTE: The IACUC provides oversight for enrollment in the OHSP related to animal subjects. IACUC-approved animal use privileges are contingent upon complete enrollment. Failure to re-enroll will result in administrative removal as a participant in associated Proposals and thereby loss of animal use privileges. Failure of Principal Investigator enrollment or re-enrollment will result in administrative inactivation of all pertinent Proposals and thereby loss of animal use privileges for the laboratory. The CMRU also assists in monitoring by controlling facility access; access into central vivaria is not granted until individuals have completed the enrollment process (i.e., completion of OHS, Level II Training and the Periodic Animal Contact Health Survey). The CMRU will also be instructed to remove access to animal facilities for individuals failing to re-enroll.

See the IACUC policies "Individual Risk Assessment and Medical Surveillance" and "Required Training" for further information about OHSP. Note: Some activities may require general safety training provided by DEHS, such as General Laboratory Safety / Hazardous Waste Training, General Bloodborne Pathogen Training, and Radiation Training.

The IACUC Participant Training Logis used to document species- and procedure-specific training; each animal user must maintain an IACUC Participant Training Log. This Log should describe prior experience for which specific procedure proficiency was obtained and outline each newly-acquired training. IACUC Participant Training Logs must be made available to the IACUC upon request. Principal Investigators are responsible for ensuring that training is obtained and documented prior to a Participant performing any animal-related procedures unsupervised. Participants conducting procedure for which the Log does not record an appropriate level of training may also lose animal use privileges. Note: Users can now assign editors to their training log. Instructions on how to assign editing or viewing privileges is available HERE.

Effective February 1, 2019: All laboratory personnel responsible for euthanasia must attend a hands-on euthanasia training session with CMRU faculty and/or staff. The IACUC is also offering the opportunity for key laboratory personnel to become CMRU-certified euthanasia trainers to train individuals within their lab; lab personnel wanting to become CMRU-certified euthanasia trainers must attend a hands-on euthanasia trainer certification course with CMRU vet staff. The CMRU offers training monthly, as well as on an individualized basis, for both the euthanasia course and the euthanasia trainer certification course. All new Proposals, 3-year renewal Proposals, and study participant additions will be subject to this euthanasia training requirement. Further information is available in the IACUC policy, "Required Training."

Information regarding available training sessions is available HERE.

CMRU-certified euthanasia trainers should document any training sessions they conduct with the Euthanasia Training Sign-In Sheet and promptly email the IACUC Office after the sessions with the sign-in sheet of participants and species-specific methods of euthanasia taught.