February 2022 Member Publications
- Xie W, Tackett AP, Berlowitz JB, Harlow AF, Kathuria H, Galiatsatos P, Fetterman JL, Cho J, Blaha MJ, Hamburg NM, Robertson RM, DeFilippis AP, Hall ME, Bhatnagar A, Benjamin EJ, Stokes AC. Association of Electronic Cigarette Use with Respiratory Symptom Development among US Young Adults. Am J Respir Crit Care Med. 2022 Jan 28. doi: 10.1164/rccm.202107-1718OC. Epub ahead of print. PMID: 35089853.
Impact Statement: This study aimed to determine whether e-cigarette use is associated with the development of respiratory symptoms in young adults. The research found that former and current e-cigarette use was associated with higher odds of developing wheezing-related respiratory symptoms, after accounting for cigarette smoking and other combustible tobacco product use.
- Cohen JE, Krishnan-Sarin S, Eissenberg T, Gould TJ, Berman ML, Bhatnagar A, Barnett TE, Soule E, Popova L, Tan ASL, Blank MD, Ling PM, O'Connor R. Balancing Risks and Benefits of E-Cigarettes in the Real World. Am J Public Health. 2022 Feb;112(2):e1-e2. doi: 10.2105/AJPH.2021.306607. PMID: 35080942; PMCID: PMC8802586.
Impact Statement: This publication is a review of Balfour's article entitled "Balancing Consideration of the Risks and Benefits of E-Cigarettes". We challenge the public health and scientific community to move away from characterizing scientists as “opponents” or “supporters” of e-cigarettes.
- Zhang H, Wang Y, Men H, Zhou W, Zhou S, Liu Q, Cai L. CARD9 Regulation and its Role in Cardiovascular Diseases. Int J Biol Sci. 2022 Jan 1;18(3):970-982. doi: 10.7150/ijbs.65979. PMID: 35173530; PMCID: PMC8771857.
Impact Statement: CARD9 is an adaptor protein expressed on myeloid cells and located downstream of pattern recognition receptors (PRRs), which transduces signals involved in innate immunity, has been extensively investigated in various fungal diseases. However, this review summarized the information for its regulating role in activating p38 MAPK, NF-κB, and NLRP3 inflammasome in various CVDs via promoting the production of proinflammatory cytokines and chemokines, leading to cardiac remodeling and dysfunction.
- Sundaram K, Mu J, Kumar A, Behera J, Lei C, Sriwastva MK, Xu F, Dryden GW, Zhang L, Chen S, Yan J, Zhang X, Park JW, Merchant ML, Tyagi N, Teng Y, Zhang HG. Garlic exosome-like nanoparticles reverse high-fat diet induced obesity via the gut/brain axis. Theranostics. 2022 Jan 1;12(3):1220-1246. doi: 10.7150/thno.65427. PMID: 35154484; PMCID: PMC8771565.
Impact Statement: Obesity is a global epidemic and reversing obesity induced chronic inflammation including brain inflammation is a hallmark of obesity via the gut-brain axis. This study demonstrated that garlic exosome-like nanoparticles (GaELNs) that inhibit systemic as well as brain inflammatory activity and reverse a HFD induced obesity in mice. These results demonstrate how nanoparticles from a healthy diet can inhibit unhealthy high-fat diet induced brain inflammation and reveal a link between brain microglia/diet to brain inflammatory disease outcomes via diet-derived exosome-like nanoparticles.
- Salazar-González RA, Doll MA, Hein DW. Arylamine N-Acetyltransferase 1 Activity is Regulated by the Protein Acetylation Status. Front Pharmacol. 2022 Jan 27;13:797469. doi: 10.3389/fphar.2022.797469. PMID: 35153780; PMCID: PMC8828969.
Impact Statement: Histone deacetylases (HDACs) and Sirtuins (SIRT) may have an important role on the N-acetyltransferase 1 (NAT1) acetylation status, affecting its catalytic capacity and having an impact on the downstream functions of this protein. We investigated the contribution of Lysine deacetylases (KDAC) in the catalytic activity of NAT1 using two different breast cancer cell lines: MDA-MB-231 (ER-, PR-, HER2-) and ZR-75-1 (ER+, PR+, HER2+) and tested the ability of Sirtuin and HDAC inhibitors to impact the transcription, translation and function of NAT1. We transiently knocked down (KD) Sirtuin 1/2 and evaluated the role of these specific proteins in the same experimental outcomes. The results show that the acetylation status of NAT1 is an important factor that might have a relevant role in the progression of cancer.
- Doll MA, Ray AR, Salazar-González RA, Shah PP, Vega AA, Sears SM, Krueger AM, Hong KU, Beverly LJ, Hein DW. Deletion of arylamine N-acetyltransferase 1 in MDA-MB-231 human breast cancer cells reduces primary and secondary tumor growth in vivo with no significant effects on metastasis. Mol Carcinog. 2022 Feb 8. doi: 10.1002/mc.23392. Epub ahead of print. PMID: 35133049.
Impact Statement: Previous studies showed that inhibition or depletion of N-acetyltransferase (NAT1) in human breast cancer cells diminishes anchorage-independent growth in culture, suggesting that NAT1 contributes to breast cancer growth and metastasis. Metastasis of parental and NAT1 knockout (KO) cells was investigated in vivo in a mouse xenograft model. NAT1 KO MDA-MB-231 cells produced primary tumors smaller than those formed by parental cells, which was contributed by an increased rate of apoptosis in KO cells. The frequency of lung metastasis, however, was not altered in NAT1 KO cells suggesting that NAT1 contributes to primary and secondary tumor growth in vivo in MDA-MB-231 breast cancer cells but does not appear to affect its metastatic potential.
- Nathala P, Salunkhe V, Samanapally H, Xu Q, Furmanek S, Fahmy OH, Deepti F, Glynn A, McGuffin T, Goldsmith DC, Petrey J, Ali T, Titus D, Carrico R, Ramirez J, Antimisiaris D, Clifford SP, Pahwa S, Roser L, Kong M, Huang J; Center of Excellence for Research in Infectious Diseases (CERID) Coronavirus Study Group on behalf of the COVID-19 Cardiovascular Research Group (COVID-CVRG). Electrocardiographic Features and Outcome: Correlations in 124 Hospitalized Patients With COVID-19 and Cardiovascular Events. J Cardiothorac Vasc Anesth. 2022 Jan 13:S1053-0770(22)00042-8. doi: 10.1053/j.jvca.2022.01.011. Epub ahead of print. PMID: 35165040; PMCID: PMC8757653.
Impact Statement: In hospitalized patients with COVID-19 and cardiovascular events, electrocardiogram at various stages of COVID-19 hospitalization showed significantly different features with dissimilar clinical outcome correlations.
- Xu Q, Liu Y, Cepulis D, Jerde A, Sheppard RA, Reichle W, Scott L, Oppy L, Stevenson G, Bishop S, Clifford SP, Liu P, Kong M, Huang J. Hand Hygiene Behaviours Monitored by an Electronic System in the Intensive Care Unit-A Prospective Observational Study. J Hosp Infect. 2022 Feb 2:S0195-6701(22)00032-9. doi: 10.1016/j.jhin.2022.01.017. Epub ahead of print. PMID: 35122887.
Impact Statement: Patterns of healthcare workers’ hand hygiene behaviors are complex and variable, which could facilitate targeted and personalized interventions to improve hand hygiene compliance.
- Zhang G, Kang Y, Cathey D, LeBlanc AJ, Cai J, Cai L, Wang S, Huang J, Keller BB. Sulforaphane Does Not Protect Right Ventricular Systolic and Diastolic Functions in Nrf2 Knockout Pulmonary Artery Hypertension Mice. Cardiovasc Drugs Ther. 2022 Feb 14. doi: 10.1007/s10557-022-07323-1. Epub ahead of print. PMID: 35157168.
Impact Statement: Nrf2 is essential in sulforaphane-mediated prevention of right ventricular dysfunction and pulmonary hypertension. Increasing Nrf2 activity in patients by supplements or intakes of vegetables may have therapeutic potential for pulmonary hypertension.
- Tracy EP, Steilberg V, Rowe G, Benson D, Nunes SS, Hoying J, Murfee WL, LeBlanc AJ. State of the Field: Cellular Therapy Approaches in Microvascular Regeneration. Am J Physiol Heart Circ Physiol. 2022 Feb 18. doi: 10.1152/ajpheart.00674.2021. Epub ahead of print. PMID: 35179976.
Impact Statement: This is a comprehensive review that gives the most recent update on cellular and exosomal therapeutic approaches in treating vascular and microvascular dysfunction. Relevant and recent clinical and animal studies utilizing cellular therapies to restore vascular function are highlighted throughout the text, and the figures and tables provided in the review are particularly helpful visual representations and also an excellent reference guide.
- Cummins TD, Korte EA, Bhayana S, Merchant ML, Barati MT, Smoyer WE, Klein JB. Advances in proteomic profiling of pediatric kidney diseases. Pediatr Nephrol. 2022 Feb 26. doi: 10.1007/s00467-022-05497-2. Epub ahead of print. PMID: 35220505.
Impact Statement: Early detection of adult and pediatric CKD and detailed mechanistic understandings of the kidney damage may be helpful in delaying or curtailing disease progression. Clinically, serum creatinine and albumin levels can be indicative of CKD, but often are a lagging indicator only significantly affected once kidney function has severely diminished. The evolution of proteomics and mass spectrometry technologies has begun to provide a powerful research tool in defining these mechanisms and identifying novel biomarkers of CKD. This review addresses the potential for improving the clinical toolkit toward better treatment of pediatric kidney diseases aided by current and future development of proteomic applications.
- Kumar A, Sundaram K, Teng Y, Mu J, Sriwastva MK, Zhang L, Hood JL, Yan J, Zhang X, Park JW, Merchant ML, Zhang HG. Ginger nanoparticles mediated induction of Foxa2 prevents high-fat diet-induced insulin resistance. Theranostics. 2022 Jan 1;12(3):1388-1403. doi: 10.7150/thno.62514. PMID: 35154496; PMCID: PMC8771553.
Impact Statement: High-fat diets (HFD) are known to increase the risk of obesity and type 2 diabetes. While diet manipulation is a foundation of prevention and treatment of obesity and diabetes, the molecular mechanisms mediating diet-based prevention of insulin resistance remain open. Here, we report that treatment with orally administered ginger-derived nanoparticles (GDNP) prevents insulin resistance by restoring homeostasis in gut epithelial Foxa2 mediated signaling in mice fed a high-fat diet (HFD).
- Smith HAB, Besunder JB, Betters KA, Johnson PN, Srinivasan V, Stormorken A, Farrington E, Golianu B, Godshall AJ, Acinelli L, Almgren C, Bailey CH, Boyd JM, Cisco MJ, Damian M, deAlmeida ML, Fehr J, Fenton KE, Gilliland F, Grant MJC, Howell J, Ruggles CA, Simone S, Su F, Sullivan JE, Tegtmeyer K, Traube C, Williams S, Berkenbosch JW. 2022 Society of Critical Care Medicine Clinical Practice Guidelines on Prevention and Management of Pain, Agitation, Neuromuscular Blockade, and Delirium in Critically Ill Pediatric Patients With Consideration of the ICU Environment and Early Mobility. Pediatr Crit Care Med. 2022 Feb 1;23(2):e74-e110. doi: 10.1097/PCC.0000000000002873. PMID: 35119438.
Impact Statement: The Society of Critical Care Medicine Practice guidelines for managing pediatric patients who require pain relief, sedation and other medications provides guidance to keep the patients comfortable and safe. These guidelines provide comprehensive clinical practice guidance for those caring for critically ill infants and children with one of the goals being to prevent adverse and long term effects from these medications.
- Coleman CJ, Yeager RA, Pond ZA, Riggs DW, Bhatnagar A, Arden Pope C 3rd. Mortality risk associated with greenness, air pollution, and physical activity in a representative U.S. cohort. Sci Total Environ. 2022 Feb 14;824:153848. doi: 10.1016/j.scitotenv.2022.153848. Epub ahead of print. PMID: 35176374.
Impact Statement: This study found that physical inactivity and exposure to air pollution were highly associated with mortality risk and that risk was especially lower among highly active individuals in areas with higher greenness. This study contributes evidence that greener environments are associated with lower risk of mortality and may help ameliorate the effects of exposure to air pollution, especially among highly active individuals.