November 2021 Member Publications
Banerjee M, Al-Eryani L, Srivastava S, Rai SN, Pan J, Kalbfleisch TS, States JC. Delineating the Effects of Passaging and Exposure in a Longitudinal Study of Arsenic-Induced Squamous Cell Carcinoma in a HaCaT Cell Line Model. Toxicol Sci. 2021 Nov 3:kfab129. doi: 10.1093/toxsci/kfab129. Epub ahead of print. PMID: 34730829.
Impact Statement: This work provides strong evidence of the requirement for passage matched controls in long term (chronic) exposure studies in cultured cells. Using passage matched controls identifies changes in gene expression that are related to the passage of time and not to the exposure, thus reducing artifacts that otherwise would be interpreted as due to the exposure.
- Petriello MC, Mottaleb MA, Serio TC, Balyan B, Cave MC, Pavuk M, Birnbaum LS, Morris AJ. Serum concentrations of legacy and emerging per- and polyfluoroalkyl substances in the Anniston Community Health Surveys (ACHS I and ACHS II). Environ Int. 2021 Nov 8;158:106907. doi: 10.1016/j.envint.2021.106907. Epub ahead of print. PMID: 34763231.
Impact Statement: We participate in a longstanding multicenter and interagency collaboration examining the metabolic health effect of PCB and dioxin exposures in residents living near a former PCB production facility in Anniston, Alabama. PFAS exposures have also been associated with metabolic diseases. Here we determined higher concentrations of PFAS in Anniston participants than the general US residential population. This finding will allow us to examine associations between PFAS exposures and previously collected disease biomarkers in this cohort.
- Jin L, Conklin DJ. A novel evaluation of endothelial dysfunction ex vivo: "Teaching an Old Drug a New Trick". Physiol Rep. 2021 Nov;9(21):e15120. doi: 10.14814/phy2.15120. PMID: 34755498; PMCID: PMC8579072.
Impact Statement: Endothelial dysfunction (ED) is sine qua non of atherosclerosis and other cardiovascular diseases in humans, yet the mechanisms that contribute to ED are still being investigated. Our study demonstrates a novel methodological twist using an old drug (L-NAME) to screen for ED in murine aorta following acute exposure to toxicants including air pollution, aldehydes, and tobacco-derived aerosols. This approach complements currently existing tools providing greater insight into the mechanisms of vascular injury due to environmental toxicants.
- Leggett CS, Doll MA, Salazar-González RA, Habil MR, Trent JO, Hein DW. Identification and characterization of potent, selective, and efficacious inhibitors of human arylamine N-acetyltransferase 1. Arch Toxicol. 2021 Nov 16. doi: 10.1007/s00204-021-03194-x. Epub ahead of print. PMID: 34783865.
Impact Statement: The study identifies potent, selective, and efficacious inhibitors of human arylamine N-acetyltransferase 1 (NAT1) important in the activation of arylamine carcinogens. The ability to inhibit NAT1 could reduce breast cancer metastasis particularly to bone.
- Wang L, Cai H, Wang Y, Liu J, Chen T, Liu J, Huang J, Guo Q, Zou W. Enhanced recovery after elective craniotomy: A randomized controlled trial. J Clin Anesth. 2022 Feb;76:110575. doi: 10.1016/j.jclinane.2021.110575. Epub 2021 Nov 2. PMID: 34739947.
Impact Statement: Implementation of an enhanced recovery after elective craniotomy protocol had significant benefits over conventional perioperative management. It was associated with a significant reduction in postoperative length of stay, medical cost, and postoperative complications.
- Polivka BJ, Eldeirawi K, Huntington-Moskos L, Nyenhuis SM. Mask Use Experiences, COVID-19, and Adults with Asthma: A Mixed-Methods Approach. J Allergy Clin Immunol Pract. 2021 Nov 14:S2213-2198(21)01262-9. doi: 10.1016/j.jaip.2021.10.071. Epub ahead of print. PMID: 34785392; PMCID: PMC8590602.
Impact Statement: This study reports that most adults with asthma reported wearing a mask in public places. Lower asthma control and longer daily mask wearing were associated with more self-reported problems with wearing masks. To increase wearability, study participants recommended using a comfortable mask that fits, staying calm, breathing slowly and deeply were recommended. Recommendations also included taking safe “mask breaks” when needed.
- Kotla NG, Isa ILM, Rasala S, Demir S, Singh R, Baby BV, Swamy SK, Dockery P, Jala VR, Rochev Y, Pandit A. Modulation of Gut Barrier Functions in Ulcerative Colitis by Hyaluronic Acid System. Adv Sci (Weinh). 2021 Nov 10:e2103189. doi: 10.1002/advs.202103189. Epub ahead of print. PMID: 34761543.
Impact Statement: This study was accomplished in collaboration (lead the project) with Drs. Yury Rochev, Abhay Pandit, CÚRAM, SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway, H91 W2TY, Ireland. The goal of this study is to discover the compounds and mechanisms that can enhance gut barrier function in ulcerative colitis conditions. Current study showed that treatment with biphasic hyaluronan (HA) enema suspension, naïve-HA systems protect against gut barrier dysfunction in ulcerative colitis by increasing tight junction proteins. These studies provide novel approaches to target ulcerative colitis.
- Lindsey ML, LeBlanc AJ, Ripplinger CM, Carter JR, Kirk JA, Hansell Keehan K, Brunt KR, Kleinbongard P, Kassiri Z. Reinforcing rigor and reproducibility expectations for use of sex and gender in cardiovascular research. Am J Physiol Heart Circ Physiol. 2021 Nov 1;321(5):H819-H824. doi: 10.1152/ajpheart.00418.2021. Epub 2021 Sep 15. PMID: 34524922.
Impact Statement: This is an editorial from the Editor-in-chief and the 7 Associate Editors of the American Journal of Physiology Heart and Circulatory Physiology that states our expectation that males and females be incorporated into all studies unless a very strong rationale prevents the authors from doing so, starting in 2023. Some examples of rationale include examining processes that occur only in one sex (i.e. cardiovascular complications of pregnancy), or testing the effects of oral contraceptives on cardiovascular health. By including studies utilizing both sexes/genders, this will result in a more complete, unbiased and reliable data set that more accurately mirrors the population.
- Schneider G, Kaliappan A, Nguyen TQ, Buscaglia R, Brock GN, Hall MB, DeSpirito C, Wilkey DW, Merchant ML, Klein JB, Wiese TA, Rivas-Perez HL, Kloecker GH, Garbett NC. The Utility of Differential Scanning Calorimetry Curves of Blood Plasma for Diagnosis, Subtype Differentiation and Predicted Survival in Lung Cancer. Cancers (Basel). 2021 Oct 23;13(21):5326. doi: 10.3390/cancers13215326. PMID: 34771491; PMCID: PMC8582427.
Impact Statement: The recent increase in the number of lung cancer cases unrelated to the well-known risk factors, and the high false-positive rate of low-dose CT scans, indicate a need to develop new, non-invasive methods for lung cancer detection. Our results using differential scanning calorimetry (DSC) supported by targeted proteomic studies suggests the DSC curve features could be useful for differentiation of lung cancer patients from controls with some capable of distinction between subtypes and stages of lung cancer.
- Trace ME, Feygin YB, Williams PG, Winders Davis D, Brothers KB, Sullivan JE, Calhoun AW. Attention-Deficit/Hyperactivity Disorder Practice Patterns: A Survey of Kentucky Pediatric Providers. J Dev Behav Pediatr. 2021 Nov 17. doi: 10.1097/DBP.0000000000001037. Epub ahead of print. PMID: 34799539.
Impact Statement: Kentucky ranks among the highest in the nation for attention-deficit/hyperactivity disorder (ADHD) prevalence in children aged 4 to 17 years. In 2011, the American Academy of Pediatrics (AAP) released a clinical practice guideline based on the DSM-IV. A guideline revision based on the Diagnostic and Statistical Manual of Mental Disorders-Fifth Edition (DSM-5) was released in October 2019. In this study, we assessed and described a sample of Kentucky pediatric providers' ADHD practices using the 2011 guideline and DSM-5 diagnostic criteria. Although pediatricians in the KY AAP that responded adhere to the DSM-5 criteria for diagnosing ADHD, pediatric providers' practices would benefit from education in improvements in pharmacotherapy titration, surveillance of coexisting conditions associated with ADHD, discussion of psychosocial interventions, and school support strategies.