Department of Biochemistry and Molecular Biology

Professor

Email Dr. Gray
Phone Number: 502-852-5226
Fax: 502-852-6222

Address: 319 Abraham Flexner Way
Louisville, KY 40202

PhD, 1968, Florida State University

Research Interests:

My research deals with the biochemistry of zinc metalloproteinases and their inhibitors. I am currently investigating the interaction between the serralysins, a family of bacterial metalloproteinases, and their endogenous inhibitors. I am also interested in the biochemistry of the matrix metalloproteinases.

Recent Publication:

Feltzer R.E., Trent J.O., and Gray R.D., Alkaline Proteinase Inhibitor Of Pseudomonas aeruginosa: A Mutational and Molecular Dynamics Study of the Role of N-Terminal Residues in the Inhibition Of Pseudomonas Alkaline Proteinase. J. Biol. Chem. 278: 25952, 2003.

Hege T., Feltzer R.E., Gray R.D., and Baumann U., Crystal Structure of a Complex between Pseudomonas aeruginosa Alkaline Protease and Its Cognate Inhibitor. Inhibition by a Zinc-NH2 Coordinative Bond. J. Biol. Chem. 276: 35087, 2001.

Feltzer R.E., Gray R.D., Dean W.L., and Pierce W.M., Jr., Alkaline Proteinase Inhibitor of Pseudomonas aeruginosa. Interaction of Native and N-Terminally Truncated Inhibitor Proteins with Pseudomonas Metalloproteinases. J. Biol. Chem. 275: 21002, 2000.

Zhang Y., Dean W.L., and Gray R.D., Cooperative Binding of Ca2+ to Human Interstitial Collagenase Assessed by Circular Dichroism, Fluorescence, and Catalytic Activity. J. Biol. Chem. 272: 1444, 1997.

Zhang Y. and Gray R.D., Characterization of Folded, Intermediate, and Unfolded States of Recombinant Human Interstitial Collagenase. J. Biol. Chem. 271: 8015, 1996.