Jonathan B. Chaires, Ph.D.

Education:

B.A., Biology (1972) University of California, Santa Cruz, CA
Physiology Course (1972 & 1973), Marine Biological Laboratory, Woods Hole, MA
Ph.D., Biophysics (1978) University of Connecticut , Storrs, CT
NIH Postdoctoral Fellow (1979 -1981) Yale University, New Haven , CT

Curriculum Vitae

Current Positions:

James Graham Brown Chair in Cancer Biophysics (2004-present), University of Louisville, Louisville, KY
Professor with Tenure (2004 – present) Department of Medicine,  University of Louisville (Louisville, KY)
Senior Scientist (July, 2004 –present), James Graham Brown Cancer Center, Louisville, KY
Professor (Joint) (2004 – present), Department of Biochemistry & Molecular Biology, University of Louisville,     Louisville, KY

Contact Information:

Clinical Translational Research Building, Room 220
University of Louisville
505 Hancock St.
Louisville, KY 40202, USA
Phone 502-852-1172
Fax 502-852-7979

Email: j.chaires@louisville.edu

Research Description:

My laboratory is interested in the biophysical chemistry of nucleic acids and their interactions.  Current interest is focused on the structure, stability and folding kinetics of G-quadruplex structures.  G-quadruplexes are four-stranded DNA structures found in telomeres and in gene promoters.  They are functionally important and are emerging targets for designed drugs.

Literature Cited:

  1. R. Buscaglia, D. M. Jameson and J. B. Chaires (2012) G-quadruplex structure and stability illuminated by 2-aminopurine phasor plots. Nucleic Acids Research 40(9): 4203-4215. PMID:22241767
  2. Nichola C. Garbett and Jonathan B. Chaires (2012) Thermodynamic studies for drug design and screening. Expert Opinion in Drug Discovery 7: 299-314. PMID: 22458502
  3. Robert D. Gray and Jonathan B. Chaires (2012) Isothermal Folding of G-quadruplexes. Methods 57: 47–55 PMID:22525787
  4. Robert D. Gray, Robert Buscaglia and Jonathan B. Chaires (2012)Populated Intermediates in the Thermal Unfolding of the Human Telomeric Quadruplex. Journal of the American Chemical Society 134: 16834–16844 PMID:22989179
  5. Huy Le, M. C Miller, Robert Buscaglia, William Dean, Patrick Holt, Jonathan B. Chaires, John O. Trent (2012) Not All G-Quadruplexes are Created Equally: An Investigation of the Structural Polymorphism of the c-Myc G-Quadruplex-Forming Sequence and its Interaction with the Porphyrin meso-Tetra(N-methyl-4-pyridyl)porphine. Organic & Biomolecular Chemistry 10, 9393-9404. PMID:23108607
  6. Vu H. Le,Robert Buscaglia, Jonathan B. Chaires, and Edwin A. Lewis (2013) Modeling Complex Equilibria in Isothermal Titration Calorimetry Experiments: Estimation of Thermodynamic Parameters for Multiple Competitive Binding Processes. Analytical Biochemistry434: 233-241 PMID: 23262283
  7. Shesh N. Rai, Jianmin Pan, Alex Cambon, Jonathan B. Chaires and Nichola C. Garbett (2013) Group Classification based on High-Dimensional Data: Application to Differential Scanning Calorimetry Plasma Thermogram Analysis of Cervical Cancer and Control Samples. Open Access Medical Statistics, 2013:3 1-9
  8. Robert Buscaglia, R.D. Gray, and Jonathan B. Chaires (2013) Thermodynamic  Characterization of Human Telomere Quadruplex Unfolding. Biopolymers, 99: 1006–1018 PMID:23536479
  9. Robert Buscaglia, M. Clarke Miller, William L. Dean, Robert D. Gray, Andrew N. Lane,John O. Trent and Jonathan B. Chaires (2013) Polyethylene glycol binding alters human telomere G-quadruplex structure by conformational selection. Nucleic Acids Research41: 7934–7946 PMID:23804761
  10. Nichola C. Garbett, Michael L. Merchant, Jonathan B. Chaires and Jon B. Klein (2013)  Calorimetric analysis of the plasma proteome: identification of type I diabetes patients with early renal function decline. BBA – General Subjects 1830: 4675–4680.  PMID: 23665587
  11. Nichola C. Garbett, Michael L. Merchant, C. William Helm, Alfred B. Jenson, Jon B. Klein, and Jonathan B. Chaires (2014) Detection of cervical cancer biomarker patterns in blood plasma and urine by differential scanning calorimetry and mass spectrometry. PLoS One 9(1): e84710. doi:10.1371/journal.pone.0084710 PMID: 24416269
  12. Guoping Zhao, Yanglong Zhu, Colins O. Eno, Yanlong Liu, Lynn DeLeeuw, Joseph A. Burlison, Jonathan B. Chaires, John O. Trent, and Chi Li (2014) Activating the Pro-apoptotic Bcl-2 Protein Bax by a Small Molecule Induces Tumor Cell Apoptosis. Molecular and Cellular Biology 34(7):1198-1207.
  13. Robert D. Gray, John O. Trent and Jonathan B. Chaires (2014) Folding and Unfolding Pathways of the Human Telomeric G-Quadruplex. Journal of Molecular Biology 426: 1629-1650. http://dx.doi.org/10.1016/j.jmb.2014.01.009Featured Article with cover image and Commentary
  14. Huy Le, William Dean, Robert Buscaglia, Jonathan B. Chaires and John O. Trent (2014) An Investigation of G-Quadruplex Structural Polymorphism in the Human Telomere Using a Combined Approach of Hydrodynamic Bead Modeling and Molecular Dynamics Simulation. Journal of Physical Chemistry B118:5390-5405.
  15. Jonathan B. Chaires, John O. Trent, Robert D. Gray, William L. Dean, Robert Buscaglia, Shelia D. Thomas, Donald M. Miller (2014) An Improved Model for the hTERTPromoter Quadruplex. Plos One9: e115580 doi:10.1371/journal.pone.0115580

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