Marsha Cole, Ph.D.

Assistant Professor

Department of Biochemistry and Molecular Biology

Baxter II, 102C, 580 S. Preston St. 502-852-7157 502-852-2558 (lab)

Research Interests

Fatty Acid Transduction of Nitric Oxide Signaling Dr. Cole's research focuses on anti-inflammatory cell signaling related to obesity, metabolic disease and subsequent cardiovascular injury. Hyperglycemic and hyperlipidemic conditions support the presence of a pro-oxidative milieu, which can lead to increased mitochondrial production of reactive oxygen species and nitric oxide synthase activity. In particular, endogenous modification of fatty acids occurs through nitric oxide-dependent oxidation and nitration in heart mitochondria. These adaptive cell signaling mediators, through posttranslational protein modification and activation of phase II enzyme expression, can potentially attenuate hyperglycemia and hyperglycemic-induced myocardial injury.

Lab Personnel

Kellianne Piell

Patrick Van Hoose

Natia Qipshidze

Megan Caroway

Shyamali Mukerjee

Selected Publications

Bonacci G, Baker PRS, Salvatore SR, Shores D, Khoo NKH, Koenitzer JR, Vitturi DA, Woodcock SR, Golin-Bisello F, Cole MP, Watkins S, St. Croix C, Batthyany CI, Freeman BA and Schopfer FJ.  Conjugated linoleic acid is a preferential substrate for fatty acid nitration.  J Biol Chem, 287(53):44071-44082, 2012.

Jung SB, Kim CS, Naqvi A, Yamamori T, Mattagajasingh I, Hoffman TA, Cole MP, Kumar A, Dericco JS, Jeon BH, Irani K. (2010) Histone deacetylase 3 antagonizes aspirin-stimulated endothelial nitric oxide production by reversing aspirin-induced lysine acetylation of endothelial nitric oxide synthase. Circ Res. 2010 Oct 1;107(7):877-87. Epub 2010 Aug 12. PMID: 20705923 [PubMed - indexed for MEDLINE]

Zhang J, Villacorta L, Chang L, Fan Z, Hamblin M, Zhu T, Chen CS, Cole MP, Schopfer FJ, Deng CX, Garcia-Barrio MT, Feng YH, Freeman BA, Chen YE. (2010) Nitro-oleic acid inhibits angiotensin II-induced hypertension. Circ Res. 2010 Aug 20;107(4):540-8. Epub 2010 Jun 17. PMID: 20558825 [PubMed - indexed for MEDLINE]

Groeger AL, Cipollina C, Cole MP, Woodcock SR, Bonacci G, Rudolph TK, Rudolph V, Freeman BA, Schopfer FJ. (2010) Cyclooxygenase-2 generates anti-inflammatory mediators from omega-3 fatty acids. Nat Chem Biol. 2010 Jun;6(6):433-41. Epub 2010 May 2. PMID: 20436486 [PubMed - indexed for MEDLINE]

Rudolph TK, Rudolph V, Edreira MM, Cole MP, Bonacci G, Schopfer FJ, Woodcock SR, Franek A, Pekarova M, Khoo NK, Hasty AH, Baldus S, Freeman BA. (2010) Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol. 2010 May;30(5):938-45. Epub 2010 Feb 18. PMID: 20167658 [PubMed - indexed for MEDLINE]

Schopfer FJ, Cole MP, Groeger AL, Chen CS, Khoo NK, Woodcock SR, Golin-Bisello F, Motanya UN, Li Y, Zhang J, Garcia-Barrio MT, Rudolph TK, Rudolph V, Bonacci G, Baker PR, Xu HE, Batthyany CI, Chen YE, Hallis TM, Freeman BA. Covalent peroxisome proliferator-activated receptor gamma adduction by nitro-fatty acids: selective ligand activity and anti-diabetic signaling actions. J Biol Chem. 2010 Apr 16;285(16):12321-33. Epub 2010 Jan 22. PMID: 20097754 [PubMed - indexed for MEDLINE]

Borniquel S, Jansson EA, Cole MP, Freeman BA, Lundberg JO. (2010) Nitrated oleic acid up-regulates PPARgamma and attenuates experimental inflammatory bowel disease. Free Radic Biol Med. 2010 Feb 15;48(4):499-505. Epub 2009 Nov 20. PMID: 19932165 [PubMed - indexed for MEDLINE]

Khoo NK, Rudolph V, Cole MP, Golin-Bisello F, Schopfer FJ, Woodcock SR, Batthyany C, Freeman BA. (2010) Activation of vascular endothelial nitric oxide synthase and heme oxygenase-1 expression by electrophilic nitro-fatty acids. Free Radic Biol Med. 2010 Jan 15;48(2):230-9. Epub 2009 Oct 24. PMID: 19857569 [PubMed - indexed for MEDLINE]

Rudolph V, Rudolph TK, Schopfer FJ, Bonacci G, Woodcock SR, Cole MP, Baker PR, Ramani R, Freeman BA. (2009) Endogenous generation and protective effects of nitro-fatty acids in a murine model of focal cardiac ischaemia and reperfusion. Cardiovasc Res. 2010 Jan 1;85(1):155-66. Epub. PMID: 19666678 [PubMed - indexed for MEDLINE]

Cole MP, Rudolph TK, Khoo NK, Motanya UN, Golin-Bisello F, Wertz JW, Schopfer FJ, Rudolph V, Woodcock SR, Bolisetty S, Ali MS, Zhang J, Chen YE, Agarwal A, Freeman BA, Bauer PM. (2009) Nitro-fatty acid inhibition of neointima formation after endoluminal vessel injury. Circ Res. 2009 Nov 6;105(10):965-72. Epub 2009 Sep 24. PMID: 19797175 [PubMed - indexed for MEDLINE]

Cole MP, Freeman BA. (2009) Promotion of cardiovascular disease by exposure to the air pollutant ozone. Am J Physiol Lung Cell Mol Physiol. 2009 Aug;297(2):L205-8. Epub 2009 Jun 12. Review. No abstract available. PMID: 19525390 [PubMed - indexed for MEDLINE]

Iles KE, Wright MM, Cole MP, Welty NE, Ware LB, Matthay MA, Schopfer FJ, Baker PR, Agarwal A, Freeman BA. (2008) Fatty acid transduction of nitric oxide signaling: nitrolinoleic acid mediates protective effects through regulation of the ERK pathway. Free Radic Biol Med. 2009 Apr 1;46(7):866-75. Epub 2008 Dec 14. PMID: 19133325 [PubMed - indexed for MEDLINE]

Rudolph V, Schopfer FJ, Khoo NK, Rudolph TK, Cole MP, Woodcock SR, Bonacci G, Groeger AL, Golin-Bisello F, Chen CS, Baker PR, Freeman BA. (2008) Nitro-fatty acid metabolome: saturation, desaturation, beta-oxidation, and protein adduction. J Biol Chem. 2009 Jan 16;284(3):1461-73. Epub 2008 Nov 17. PMID: 19015269 [PubMed - indexed for MEDLINE]

Kim CS, Jung SB, Naqvi A, Hoffman TA, DeRicco J, Yamamori T, Cole MP, Jeon BH, Irani K. (2008) p53 impairs endothelium-dependent vasomotor function through transcriptional upregulation of p66shc. Circ Res. 2008 Dec 5;103(12):1441-50. Epub 2008 Nov 6. PMID: 18988897 [PubMed - indexed for MEDLINE]

Kiningham KK, Cardozo ZA, Cook C, Cole MP, Stewart JC, Tassone M, Coleman MC, Spitz DR. (2008) All-trans-retinoic acid induces manganese superoxide dismutase in human neuroblastoma through NF-kappaB. Free Radic Biol Med. Apr 15;44(8):1610-6. Epub 2008 Jan 30. PMID: 18280257 [PubMed - indexed for MEDLINE]

Nithipongvanitch R, Ittarat W, Cole MP, Tangpong J, Clair DK, Oberley TD. (2007) Mitochondrial and nuclear p53 localization in cardiomyocytes: redox modulation by doxorubicin (Adriamycin)? Antioxid Redox Signal. 2007 Jul;9(7):1001-8. Review. PMID: 17508921 [PubMed - indexed for MEDLINE]

Spasojevic I, Chen Y, Noel TJ, Yu Y, Cole MP, Zhang L, Zhao Y, St Clair DK, Batinic-Haberle I. (2007) Mn porphyrin-based superoxide dismutase (SOD) mimic, MnIIITE-2-PyP5+, targets mouse heart mitochondria. Free Radic Biol Med. 2007 Apr 15;42(8):1193-200. Epub 2007 Jan 13. PMID: 17382200 [PubMed - indexed for MEDLINE]

Tangpong J, Cole MP, Sultana R, Estus S, Vore M, St Clair W, Ratanachaiyavong S, St Clair DK, Butterfield DA. (2007) Adriamycin-mediated nitration of manganese superoxide dismutase in the central nervous system: insight into the mechanism of chemobrain. J Neurochem. 2007 Jan;100(1):191-201. PMID: 17227439 [PubMed - indexed for MEDLINE]

Jungsuwadee P, Cole MP, Sultana R, Joshi G, Tangpong J, Butterfield DA, St Clair DK, Vore M. (2006) Increase in Mrp1 expression and 4-hydroxy-2-nonenal adduction in heart tissue of Adriamycin-treated C57BL/6 mice. Mol Cancer Ther. 2006 Nov;5(11):2851-60. PMID: 17121932 [PubMed - indexed for MEDLINE]

Tangpong J, Cole MP, Sultana R, Joshi G, Estus S, Vore M, St Clair W, Ratanachaiyavong S, St Clair DK, Butterfield DA. (2006) Adriamycin-induced, TNF-alpha-mediated central nervous system toxicity. Neurobiol Dis. 2006 Jul;23(1):127-39. Epub 2006 May 11. PMID: 16697651 [PubMed - indexed for MEDLINE]

Cole MP, Chaiswing L, Oberley TD, Edelmann SE, Piascik MT, Lin SM, Kiningham KK, St Clair DK. (2005) The protective roles of nitric oxide and superoxide dismutase in adriamycin-induced cardiotoxicity. Cardiovasc Res. 2006 Jan;69(1):186-97. Epub 2005 Sep 12. PMID: 16157314 [PubMed - indexed for MEDLINE] Free Article

Chaiswing L, Cole MP, Ittarat W, Szweda LI, St Clair DK, Oberley TD. (2005) Manganese superoxide dismutase and inducible nitric oxide synthase modify early oxidative events in acute adriamycin-induced mitochondrial toxicity. Mol Cancer Ther. 2005 Jul;4(7):1056-64. PMID: 16020663 [PubMed - indexed for MEDLINE] Free Article

Humphrey ML, Cole MP, Pendergrass JC, Kiningham KK. (2005) Mitochondrial mediated thimerosal-induced apoptosis in a human neuroblastoma cell line (SK-N-SH). Neurotoxicology. 2005 Jun;26(3):407-16. PMID: 15869795 [PubMed - indexed for MEDLINE]

Chaiswing L, Cole MP, St Clair DK, Ittarat W, Szweda LI, Oberley TD. (2004) Oxidative damage precedes nitrative damage in adriamycin-induced cardiac mitochondrial injury. Toxicol Pathol. 2004 Sep-Oct;32(5):536-47. PMID: 15605432 [PubMed - indexed for MEDLINE] Free Article