Marsha Cole, Ph.D.

Assistant Professor

Department of Biochemistry and Molecular Biology


Baxter II, 102C, 580 S. Preston St. 502-852-7157 502-852-2558 (lab) mpcole01@louisville.edu

Research Interests

  • Biochemical events related to the occurrence, progression and prevention of heart injury (and myocardial infarction)

  • Impaired mitochondrial function in obesity- and age-induced cardiac injury

My research is focused on the role of redox signaling in oxidative cardiac injury and heart disease.  Mitochondria are unique organelles that carry out specialized functions, but in addition to being a source of energy, they are also a primary source of reactive oxygen species (ROS) generation. Since mitochondria are abundant in cardiac tissue and control cell cycle/growth, they are typically implemented in numerous diseases and inflammatory models, including cardiovascular dysfunction and aging.  In particular, disease and inflammation support the presence of a pro-oxidative milieu, which can lead to increased mitochondrial production of ROS and nitric oxide synthase activity.  Moreover, my lab is interested in further elucidating the cell signaling and pathogenesis (and measurement) of nitric oxide (NO) and NOx, including consumption of NO via ROS, as well as NO and (nitrite)-mediated nitration of unsaturated fatty acids.

Lab Personnel

Kellianne Piell, Senior Research Technician and Lab Manager

Natia Qipshidze-Kelm, Postdoctoral Fellow

Patrick Van Hoose, Graduate Student

Linda Omer, Graduate Student

Catherine Cobb, Graduate Student

Jane Solinger, High School Student

Selected Publications

Piel, KM, Qipshidze Kelm N, Caroway MP, Aman M, Cole MP.  Nitrite treatment rescues cardiac dysfunction in aged mice treated with conjugated linoleic acid.  Free Radic Biol Med. 2014 Apr 8;72C:66-75. doi: 10.1016/j.freeradbiomed.2014.03.043. [Epub ahead of print]  PMID:  24721151

Cole MP*, Tangpong J, Oberley TD, Chaiswing L, Kiningham KK, St. Clair DK.  Nuclear interaction between ADR-induced p65 and p53 mediates cardiac injury in iNOS (-/-) mice.  PLoS One, 9(2):e89251.doi:  10.1371/journal/pone.0089251. PMID: 24586632                 *corresponding author

Kelm NQ, Piell KM, Solinger JC, Cole MP.  Combination treatment with conjugated linoleic acid and ntrite protects against myocardial infarction.  Redox Biol, 7(2):1-7, 2013. Doi:  10.1016/j.redox.2013.10.009. PMID: 24363996

Bonacci G, Baker PRS, Salvatore SR, Shores D, Khoo NKH, Koenitzer JR, Vitturi DA, Woodcock SR, Golin-Bisello F, Cole MP, Watkins S, St. Croix C, Batthyany CI, Freeman BA and Schopfer FJ.  Conjugated linoleic acid is a preferential substrate for fatty acid nitration.  J Biol Chem. 2012 Dec 28;287(53):44071-82. doi: 10.1074/jbc.M112.401356. Epub 2012 Nov 9. PMID: 23144452 [PubMed - indexed for MEDLINE] Free PMC Article

Jung S-B, Kim CS, Naqvi A, Yamamori T, Mattagajasingh I, Hoffman TA, Cole MP, Kumar A, Dericco JS, Jeon BH, Irani K. (2010) Histone deacetylase 3 antagonizes aspirin-stimulated endothelial nitric oxide production by reversing aspirin-induced lysine acetylation of endothelial nitric oxide synthase. Circ Res. 2010 Oct 1;107(7):877-87. Epub 2010 Aug 12. PMID: 20705923 [PubMed - indexed for MEDLINE]

Zhang J, Villacorta L, Chang L, Fan Z, Hamblin M, Zhu T, Chen CS, Cole MP, Schopfer FJ, Deng CX, Garcia-Barrio MT, Feng YH, Freeman BA, Chen YE. (2010) Nitro-oleic acid inhibits angiotensin II-induced hypertension. Circ Res. 2010 Aug 20;107(4):540-8. Epub 2010 Jun 17. PMID: 20558825 [PubMed - indexed for MEDLINE]

Groeger AL, Cipollina C, Cole MP, Woodcock SR, Bonacci G, Rudolph TK, Rudolph V, Freeman BA, Schopfer FJ. (2010) Cyclooxygenase-2 generates anti-inflammatory mediators from omega-3 fatty acids. Nat Chem Biol. 2010 Jun;6(6):433-41. Epub 2010 May 2. PMID: 20436486 [PubMed - indexed for MEDLINE]

Rudolph TK, Rudolph V, Edreira MM, Cole MP, Bonacci G, Schopfer FJ, Woodcock SR, Franek A, Pekarova M, Khoo NK, Hasty AH, Baldus S, Freeman BA. (2010) Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol. 2010 May;30(5):938-45. Epub 2010 Feb 18. PMID: 20167658 [PubMed - indexed for MEDLINE]

Schopfer FJ*, Cole MP*, Groeger AL*, Chen CS, Khoo NK, Woodcock SR, Golin-Bisello F, Motanya UN, Li Y, Zhang J, Garcia-Barrio MT, Rudolph TK, Rudolph V, Bonacci G, Baker PR, Xu HE, Batthyany CI, Chen YE, Hallis TM, Freeman BA. Covalent peroxisome proliferator-activated receptor gamma adduction by nitro-fatty acids: selective ligand activity and anti-diabetic signaling actions. J Biol Chem. 2010 Apr 16;285(16):12321-33. Epub 2010 Jan 22. PMID: 20097754 [PubMed - indexed for MEDLINE] *contributed equally

Joshi G, Aluise CD, Cole MP, Sultana R, Pierce WM, Vore M, St. Clair DK, Butterfield DA.  Alterations in brain antioxidant enzymes and redox proteiomic identification of oxidized brain proteins induced by the anti-cancer drug Adriamycin:  implications for oxidative stress-mediated chemobrain. Neuroscience. 2010 Mar 31;166(3):796-807. doi: 10.1016/j.neuroscience.2010.01.021. Epub 2010 Jan 20. PMID: 20096337 [PubMed - indexed for MEDLINE] Free PMC Article

Borniquel S, Jansson EA, Cole MP, Freeman BA, Lundberg JO. (2010) Nitrated oleic acid up-regulates PPARgamma and attenuates experimental inflammatory bowel disease. Free Radic Biol Med. 2010 Feb 15;48(4):499-505. Epub 2009 Nov 20. PMID: 19932165 [PubMed - indexed for MEDLINE]

Khoo NK, Rudolph V, Cole MP, Golin-Bisello F, Schopfer FJ, Woodcock SR, Batthyany C, Freeman BA. (2010) Activation of vascular endothelial nitric oxide synthase and heme oxygenase-1 expression by electrophilic nitro-fatty acids. Free Radic Biol Med. 2010 Jan 15;48(2):230-9. Epub 2009 Oct 24. PMID: 19857569 [PubMed - indexed for MEDLINE]

Rudolph V, Rudolph TK, Schopfer FJ, Bonacci G, Woodcock SR, Cole MP, Baker PR, Ramani R, Freeman BA. (2009) Endogenous generation and protective effects of nitro-fatty acids in a murine model of focal cardiac ischaemia and reperfusion. Cardiovasc Res. 2010 Jan 1;85(1):155-66. Epub. PMID: 19666678 [PubMed - indexed for MEDLINE]

Schopfer FJ, Batthyány C, Baker PRS, Bonacci G, Cole MP, Rudolph V, Rudolph TK, Nadtochiy S, Brookes PS, Freeman BA.  Detection and quantification of protein adduction by electrophilic fatty acid nitration products:  mitochondrial generation of fatty acid nitroalkene derivatives. Free Radic Biol Med. 2009 May 1;46(9):1250-9. doi: 10.1016/j.freeradbiomed.2008.12.025. PMID: 19353781 [PubMed - indexed for MEDLINE] Free PMC Article

Cole MP, Rudolph TK, Khoo NK, Motanya UN, Golin-Bisello F, Wertz JW, Schopfer FJ, Rudolph V, Woodcock SR, Bolisetty S, Ali MS, Zhang J, Chen YE, Agarwal A, Freeman BA, Bauer PM. (2009) Nitro-fatty acid inhibition of neointima formation after endoluminal vessel injury. Circ Res. 2009 Nov 6;105(10):965-72. Epub 2009 Sep 24. PMID: 19797175 [PubMed - indexed for MEDLINE]

Cole MP, Freeman BA. (2009) Promotion of cardiovascular disease by exposure to the air pollutant ozone. Am J Physiol Lung Cell Mol Physiol. 2009 Aug;297(2):L205-8. Epub 2009 Jun 12. Review. No abstract available. PMID: 19525390 [PubMed - indexed for MEDLINE]

Cole MP, Chaiswing L, Oberley TD, Edelmann SE, Piascik MT, Lin SM, Kiningham KK, St Clair DK. (2005) The protective roles of nitric oxide and superoxide dismutase in adriamycin-induced cardiotoxicity. Cardiovasc Res. 2006 Jan;69(1):186-97. Epub 2005 Sep 12. PMID: 16157314 [PubMed - indexed for MEDLINE] Free Article