Imaging

Imaging

Equipment

Image Core Facilities

The Imaging Core offers use and training on the equipment listed on tabs to left. To request and schedule use, please complete the online contract. Once the contract is approved, scheduling can proceed. Please contact Michael Eisenback, 852-5173, Cell: 502-333-4466, Email for details.

Unauthorized use will cause restriction or loss of privileges.

 

Director: Michael Eisenback, 852-5173, Cell: 502-333-4466, Email
Location: Bldg. A, Rm 1015D, 500 South Preston St., Louisville, KY 40202

Olympus FluoView™ 500 Confocal Laser Scanning Microscope

  • Fully Automated Scanning Unit; Variety of image processing and analysis functions
  • Optical sectioning, 3D construction; Time-series observation; Differential Interference Contrast
  • Automatic Laser Control (AOTF or ND) / Laser Combiner
  • Wide range of laser light sources from UV to near infrared lasers:
    • Argon, HeNeG, HeNeR
    • Wavelengths: 405nm, 488nm, 543nm, 633nm.
  • Intuitive user friendly software; High resolution: 2048 x 2048 pixels; 10-fold digital zoom

 

confocal laser scanning microscope

Two Philips CM10 Transmission Electron Microscopes

TEM microscope Phillips CM12
  • High Contrast objective lens, Single condenser lens
  • Tilt Specimen holder, 60 degrees rotation
  • 15 Mega pixel SIA Digital Camera

 

Director: Michael Eisenback, 852-5173, Cell: 502-333-4466, Email
Location: Analytical Microscopy Facility, Room 1012D, Health Sciences Tower

Two Philips CM10 Transmission Electron Microscopes

  • High Contrast objective lens, Single condenser lens
  • Tilt Specimen holder, 60 degrees rotation
  • 15 Mega pixel SIA Digital Camera
TEM microscope Phillips CM12

Director: Michael Eisenback, 852-5173, Cell: 502-333-4466, Email
Location: Lower Level, Health Sciences Center, Building A

The VEGA scanning electron microscope.

  • Configured with a tungsten heated cathode; Unique four-lens Wide Field Optics™
  • Live stereoscopic imaging, using advanced 3D Beam Technology
  • Large chamber with an extended 3 axes fully motorized compucentric stage
  • YAG-based Backscattered Detectors for excellent durability
  • High speed imaging and excellent signal to noise ratio
  • 3D metrology software for Height, Surface and Profile surface measurements
  • Built-in image management and report generation

VEGA scanning electron microscopen workstation

Director: Michael Eisenback, 852-5173, Cell: 502-333-4466, Email
Location: Room 1008, Health Sciences Center, Building A, Louisville, KY

Neurolucida system

Advanced software for neuron tracing and reconstruction, neuron analysis, and 3D brain mapping

Quantify neuronal morphology

Neurolucida is a powerful tool for creating and analyzing realistic, meaningful, and quantifiable neuron reconstructions that can perform detailed morphometric analysis of neurons, such as quantifying:

  • the number of dendrites, axons, nodes, synapses, and spines
  • the length, width, and volume of dendrites and axons
  • area and volume of the soma
  • the complexity and extension of neurons

Most of what is visible through the oculars is displayed on the computer screen. In addition to software for neuron tracing and analysis, the Neurolucida system includes a microscope, computer, motorized stage, and camera. With the joy stick remote you can:

  • control motorized stages
  • control cameras to change or save settings for future use
  • operate the focus knob or change objectives
  • change filter turrets, filter wheels, and mirror cubes when acquiring fluorescent images or image stacks

Neurolucida system

Director: Michael Eisenback, 852-5173, Cell: 502-333-4466, Email
Location: Analytical Microscopy Facility, Room 1012D, Health Sciences Tower

Digital Darkroom with both digital and X-ray film capabilities available

Request service or schedule equipment

  1. Complete the online contract form
  2. Once the contract is approved, scheduling can proceed. Please contact Michael Eisenback, 852-5173, Cell: 502-333-4466, Email for details.
  3. Unauthorized use will cause restriction or loss of privileges.

Imaging Core Facility Fees