Address:511 South Floyd, Room 111, Louisville, KY 40202
Dr. Guido's research focuses on the form and function of developing sensory systems. Of particular interest is understanding the cellular and molecular mechanisms responsible for the activity dependent refinement of sensory connections. The laboratory's model system has been the mouse lateral geniculate nucleus, the thalamic relay between retina and visual cortex. This pathway develops from a crudely wired, relatively undifferentiated network of cells into a highly ordered sensory system comprised of precise retinotopic patterns of connectivity, separate eye-specific domains, distinct cell types, and elaborate intrinsic circuitry.
A variety of in vitro electrophysiological recording techniques are utilized, including visualized whole cell patch recordings. Anatomical experiments involve the use of anterograde tracers, molecular and immunocytochemical markers, and biocytin labeled material to delineate the functional and structural organization of the developing visual system. Biochemical experiments make use of western blots and PCR to examine the molecular composition of neural elements underlying visual system development. More recently, confocal microscopy and optogenetics have been utilized to capture the dynamics of developing thalamic circuitry.
Present research activities include defining the anatomical and functional state of the developing retinogeniculate synapse, understanding the mechanisms that regulate the development of nonretinal circuitry in dLGN, using optogenetics to activate retinal and nonretinal circuits in dLGN, understanding how parallel visual channels from retina are organized in central visual targets.
Krahe, T.E., Seabrook T., Chen C-J., Fox, M.A . & Guido W* (2012) Modulation of CREB in the dorsal lateral geniculate nucleus of dark reared mice. Neural Plasticity, in press
Krahe, T.E., El-Danaf, R.N., Dilger, E.K., Henderson, S.C. & Guido, W. * (2011) Morphologically distinct classes of relay cells exhibit regional preferences in the dorsal lateral geniculate nucleus of the mouse. Journal of Neuroscience 31(48) 17437-17448.
Fox M.A. & Guido W. (2011) Shedding light on class specific wiring: development of intrinsically photosensitive retinal ganglion cell circuitry. Molecular Neuroscience, 44(3) 321-329.
Krahe T.E. & Guido W*. (2011). Homeostatic plasticity in the visual thalamus by monocular deprivation. The Journal of Neuroscience, 31(18): 6842-6849. (View PDF)
McNeill D.S., Sheely C.J., Ecker J.L., Badea T.C., Morhardt D., Guido W.* & Hattar S*. (2011). Development of melanopsin-based irradiance detecting circuitry. Neural Development, 6(1): 8. (View PDF)
Dilger, E. K., Shin, H. S., & Guido, W*. (2011). Requirements for synaptically evoked plateau potentials in relay cells of the dorsal lateral geniculate nucleus of the mouse. The Journal of Physiology, 589 (5) 1103-1115. (View PDF)
Su, J., Haner, C. V., Imbery, T. E., Brooks, J. M., Morhardt, D. R., Gorse, K., Guido W. & Fox M.A. (2011). Reelin is required for class-specific retinogeniculate targeting. The Journal of Neuroscience, 31(2), 575-586 9. (View PDF)
Bickford, M. E., Slusarczyk, A., Dilger, E. K., Krahe, T. E., Kucuk, C., & Guido, W*. (2010). Synaptic development of the mouse dorsal lateral geniculate nucleus. The Journal of Comparative Neurology, 518(5), 622-635. (View PDF)
Ziburkus, J., Dilger, E. K., Lo, F. S., & Guido, W*. (2009). LTD and LTP at the developing retinogeniculate synapse. Journal of Neurophysiology, 102(6), 3082-3090. (View PDF)
Guido, W. (2008). Refinement of the retinogeniculate pathway. The Journal of Physiology, 586, 4357-4362. (View PDF)
Guido, W. (2008). A shout out to immature synapses. focus on "different roles for AMPA and NMDA receptors in transmission at the immature retinogeniculate synapse". Journal of Neurophysiology, 99(2), 411-412. (View PDF)
Jeon, D., Song, I., Guido, W., Kim, K., Kim, E., Oh, U., et al. (2008). Ablation of Ca2+ channel beta3 subunit leads to enhanced N-methyl-D-aspartate receptor-dependent long term potentiation and improved long term memory. The Journal of Biological Chemistry, 283(18), 12093-12101. (View PDF)
Demas, J., Sagdullaev, B. T., Green, E., Jaubert-Miazza, L., McCall, M. A., Gregg, R. G., Wong R.O. & Guido W*. (2006). Failure to maintain eye-specific segregation in nob, a mutant with abnormally patterned retinal activity. Neuron, 50(2), 247-259. (View PDF)
Ziburkus, J., & Guido, W*. (2006). Loss of binocular responses and reduced retinal convergence during the period of retinogeniculate axon segregation. Journal of Neurophysiology, 96(5), 2775-2784 (View PDF)
Jaubert-Miazza, L., Green, E., Lo, F. S., Bui, K., Mills, J., & Guido, W.* (2005). Structural and functional composition of the developing retinogeniculate pathway in the mouse. Visual Neuroscience, 22(5), 661-676. (View PDF)
Carden, W. B., Guido, W., Godwin, D. W., & Bickford, M. E. (2003). Thalamocortical cells in the cat pulvinar nucleus transiently express nitric oxide synthase during development. Neuroscience Letters, 351(2), 87-90. (View PDF)
Li, J., Guido, W. & Bickford, M. E. (2003). Two distinct types of corticothalamic EPSPs and their contribution to short-term synaptic plasticity. Journal of Neurophysiology, 90(5), 3429-3440. (View PDF)
Ziburkus, J., Lo, F. S., & Guido, W.* (2003). Nature of inhibitory postsynaptic activity in developing relay cells of the lateral geniculate nucleus. Journal of Neurophysiology, 90(2), 1063-1070. (View PDF)
Li, J., Bickford, M. E., & Guido, W.* (2003). Distinct firing properties of higher order thalamic relay neurons. Journal of Neurophysiology, 90(1), 291-299. (View PDF)
Lo, F. S., Ziburkus, J., & Guido, W.* (2002). Synaptic mechanisms regulating the activation of a Ca2+ mediated plateau potential in developing relay cells of the LGN. Journal of Neurophysiology, 87(3), 1175-1185. (View PDF)
Guido, W., Lo, F. S., & Erzurumlu, R. S. (2001). Synaptic plasticity in the trigeminal principal nucleus during the period of barrelette formation and consolidation. Developmental Brain Research, 132(1), 97-102. (View PDF)
Weyand, T. G., Boudreaux, M., & Guido, W.* (2001). Burst and tonic response modes in thalamic neurons during sleep and wakefulness. Journal of Neurophysiology, 85(3), 1107-1118. (View PDF)
Scheiner, C. A., Kratz, K. E., Guido, W., & Mize, R. R. (2001). Prenatal and postnatal expression of nitric oxide in the developing kitten superior colliculus revealed with NADPH diaphorase histochemistry. Visual Neuroscience, 18(1), 43-54.
Ziburkus, J., Bickford, M. E., & Guido, W.* (2000). NMDAR-1 staining in the lateral geniculate nucleus of normal and visually deprived cats. Visual Neuroscience, 17(2), 187-196.(View PDF)
Development and Plasticity in Sensory Thalamus and Cortex, Editors R. Erzrurmulu, W. Guido, Z. Molnar, Springer, NY, 2006.