Microbiology

Our Microbiology Core facility provides support and service for a wide range or microbiological research activities, many of which complement the ongoing work within the HTB and Animal Core facilities. This includes the growth, amplification, and characterization of pathogens as well as the purification of pathogens or nucleic acids for use in diagnostic assays. Activities also include the determination of pathogen levels in association with antiviral screens, cell-based biological assays, or from animal tissues. In addition, we will work with you to develop and customize assays to specifically meet your research needs.

Available assays include but are not limited to:

  • ELISA
  • Hemagglutination/hemagglutination inhibition assays
  • Immunofluorescence assays
  • Microneutralization assays
  • PCR, RT-PCR, real-time PCR
  • Plaque assays
  • Plaque reduction neutralization assays

BSL2 Agents

Chikungunya virus
Dengue virus
Influenza virus (seasonal, mouse-adapted)
Junin virus (Candid 1)
LCMV (ARM)
Respiratory syncytial virus
Venezuelan equine encephalitis virus (TC-83)
Yellow fever virus (17D)
Zika virus
Burkholderia thailandensis
Klebsiella pnuemoniae
Pseudomonas aeruginosa (MDR)
Salmonella species
Yersinia enterocolitica
Yersinia pseudotuberculosis

BSL3 Agents

Hantaviruses
Influenza virus (H2N2)
Japanese encephalitis virus
LCMV (WE)
MERS-CoV
Mopei virus (ML29)
Western equine encephalitis virus
West Nile virus
Mycobacterium tuberculosis

BSL3 Select Agents

Influenza virus (highly-pathogenic avian H5N1)
SARS-CoV (wild-type, mouse-adapted)
Venezuelan equine encephalitis virus (Trinidad-donkey)
Eastern equine encephalitis virus
Rift Valley Fever virus
Burkholderia mallei
Burkholderia pseudomallei
Yersinia pestis

We will work with you to develop the most cost-effective assays and reagents based on your research needs. Please contact us for the most up-to-date assay and pathogen availability along with current full-service pricing information. Volume discounts available for larger quantities of tests.

William E. Severson, Ph.D.
Director, Shared Resources
Center for Predictive Medicine
Phone: (502) 852-1546
Email: William Severson

Jon Gabbard, Ph.D.
Program Manager
Center for Predictive Medicine
Phone: (502) 852-1365
Email: Jon Gabbard

General

Biosafety in Microbiological and Biomedical Laboratories, 5th Edition, CDC, an advisory document recommending best practices for the safe conduct of work in biomedical and clinical laboratories from a biosafety perspective.

CDC Import Permit Program (IPP), information relating to the importation of infectious biological agents, substances, or vectors into the US.

Federal Select Agent Program, website contains application packages and forms, current regulations regarding Select Agents, and resource information.

Virology

International Taxonomy of Viruses, an official index of virus names.

Virology Blog, a weblog to teach about viruses and viral disease.

American Society for Virology, home site for all things virology including membership information, virology jobs, and annual meeting information.

Bacteriology

American Society for Microbiology, home to the oldest and largest scientific society providing a resource for educational information, scientific journal subscriptions, annual meetings, and career information.