William M. Pierce, Ph. D.

Executive Vice President for Research & Innovation

Email Bill Pierce
Phone Number: (502)-852-7424 (Office) &  (502)-852-5161 (Lab)
Fax Number: (502)-852-7868
Address:         Department of Pharmacology and Toxicology
School of Medicine
University of Louisville
Louisville, KY 40292
Ph. D. 1981, University of Louisville

 

Additional Appointments:

  • Graduate Faculty, University of Louisville
  • Professor of Pharmacology and Toxicology, School of Medicine
  • Director, Biomolecular Mass Spectrometry Laboratory
  • Director, Therapeutics and Toxicology Laboratory, Department of Pharmacology and Toxicology,  School of Medicine
  • Professor of Chemistry,  College of Arts & Sciences
  • Special Assistant to the Provost – Strategic Planning, University of Louisville
  • Interim Vice Provost for Graduate Affairs, University of Louisville

 

Investigators:

  • Jian Cai, Ph.D., Asst. Professor of Pharmacology and Toxicology
  • Ned B. Smith, Laboratory Manager
  • Kevin G. Taylor, Senior Technologist

 

Research Interests:

  • Design of novel drugs for treatment of bone disorders and metabolic disease.
  • Uses of mass spectrometry in molecular pharmacology and toxiocology.

 

Selected Publications:

  1. Cai, J, A. Bhatnagar and WM Pierce Jr.  Protein modification by acrolein: Formation and stability of cysteine.  Chem Res Toxicol. 22:708-716, 2009.
  2. M. Perluigi, R. Sultana, G. Cenini, F. Di Domenico, M. Memo, W.M. Pierce, R. Coccia, and D. A. Butterfield, "Redox Proteomics Identification of HNE-Modified Brain Proteins in Alzheimer's Disease: Role of Lipid Peroxidation in Alzheimer's Disease Pathogenesis," Proteomics--Clinical Applications 3, 682-693 (2009)
  3. Nasima, Shama, William M. Pierce Jr.,  K. Grant Taylor. Peter A. Crooks   An Improved and Scalable Synthetic route to the Synthon, 17--(2-Carboxyethyl)-1,3,5(10)-estratriene: an Important Intermediate in the Synthesis of Bone Targeting Estrogens.  Submitted to Tetrahedron Lett., February 2009.
  4. Bendaly, J., Kristin J. Metry, Mark A. Doll, J. Christopher States, Ned B. Smith,  Jason R. Neale, Jessica L. Holloman, William M. Pierce, Jr. and David W. Hein.  Effects of human N-acetyltransferase 2 genetic polymorphism on mutagenicity and DNA adduct formation following exposures to 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine or 4-aminobiphenyl.  Submitted to Cancer Epidemiology, Biomarkers, and Prevention, June 2008.
  5. Bendaly, Jean,  Mark A. Doll, Lori M. Millner, Kristin J. Metry, Ned B. Smith, William M. Pierce, Jr. and David W. HeinDifferences between human slow N-acetyltransferase 2 alleles in DNA adduct and mutant levels from 4-aminobiphenyl.  Submitted to Carcinogenesis, April, 2009.
  6. Reed TT, Pierce WM, Markesbery WR, Butterfield DA. Proteomic identification of HNE-bound proteins in early Alzheimer disease: Insights into the role of lipid peroxidation in the progression of AD.  Brain Res. 2009 Apr 14;. [Epub ahead of print] PMID: 19374891
  7. Opii WO, Joshi G, Head E, Milgram NW, Muggenburg BA, Klein JB, Pierce WM, Cotman CW, Butterfield DA.  Proteomic identification of brain proteins in the canine model of human aging following a long-term treatment with antioxidants and a program of behavioral enrichment: Relevance to Alzheimer's disease Neurobiol Aging. Jan;29(1):51-70, 2008.
  8. Neale, JR, NB Smith, WM Pierce, Jr. and DW Hein.  Methods for aromatic and heterocyclic amine carcinogen-DNA adduct analysis by liquid chromatography-tandem mass spectrometry.  J. Polycyclic Aromatic Hydrocarbons ,28:4,402- 417 2008. PMID: 1912280
  9. Reed T, Perluigi M, Sultana R, Pierce WM, Klein JB, Turner DM, Coccia R, Markesbery WR, Butterfield DA.   Redox Proteomic Identification of 4-Hydroxy-2-Nonenal-Modified Brain Proteins in Amnestic Mild Cognitive Impairment: Insight into the Role of Lipid Peroxidation in the Progression and Pathogenesis of Alzheimer's Disease.  Neurobiology of Disease, Apr;30(1):107-20, 2008. PMID: 18325775
  10. S Datta, D Turner, R Singh, BL. Ruest, WM. Pierce Jr. and TB. Knudsen.  Fetal Alcohol Syndrome (FAS) in Mice Detected Through Proteomics Screening of the Amniotic Fluid.  Birth Defects Res A Clin Mol Teratol.  Apr;82(4):177-86, 2008.  PMID: 18240165
  11. Yang X, Luo C, Cai J, Pierce W, Tezel G. Phosphorylation-dependent interaction with 14-3-3 regulates Bad trafficking in retinal ganglion cells.   Invest Ophthalmol Vis Sci. 2008 Jun;49(6):2483-94.  PMID: 18296656
  12. Reed, Tanea; Owen, Joshua; Pierce, William; Sebastian, Andrea; Sullivan, Patrick; Butterfield, Allan.  Proteomic Identification of Nitrated Brain Proteins in Traumatic Brain Injured Rats Treated Post-Injury with Gamma-Glutamylcysteine Ethyl Ester: Insights into the Role of Glutathione Elevation as a Potential Therapeutic Strategy For Traumatic Brain Injury.    J  Neurosci Res. 2009 87(2):408-417.  PMID: 18803298
  13. Reed, TT, DM Turner, WM Pierce Jr., WR Markebury, DA Butterfield.  "Proteomic identification of nitrated brain proteins in early Alzheimer’s disease inferior parietal lobule" J Cell Mol Med  2008 Aug 21;. [Epub ahead of print] PMID: 18752637
  14. Owen JB,  WO Opii, C Ramassamy,  WM Pierce, Butterfield DA.   Proteomic analysis of brain protein expression levels in NF-kappabeta p50 (-/-) homozygous knockout mice. Brain Research 1240: 22-30, 2008.  PMID: 18817763
  15. Owen, Joshua; Di Domenico, Fabio; Sultana, Rukhsana; Perluigi, Marzia; Cini, Chiara; Pierce, William; Butterfield, D. Allan   "Proteomics-determined differences in the Concanavalin-A fractionated proteome of hippocampus and inferior parietal lobule in subjects with Alzheimer's disease and mild cognitive impairment: Implications for progression of AD"  J. Proteome Res. 8, 471-482 , 2009. PMID: 19072283
  16. Bendaly, Jean, Kristin J. Metry, Mark A. Doll, GuoHui Jiang, J. Christopher States, Ned B. Smith, Jason R. Neale, Jessica L. Holloman, William M. Pierce, Jr. and David W. Hein  Role of Human CYP1A1 and NAT2 in 2-Amino-1-methyl-6-phenylimidazo-4,5-b]pyridine-induced Mutagenicity and DNA Adducts.  Xenobiotica. 2009 Mar 19;:1-8. [Epub ahead of print]
  17. Neale, J.R. . NB Richter,  KE Merten, KG Taylor,  S Singh; LC Waite, NK Emery; NB Smith; J Cai and WM Pierce, Jr.  Bone Selective Effect of an Estradiol Conjugate with a Novel Tetracycline-derived Bone-Targeting Agent.     Bioorg Med Chem Lett. 2008 Dec 24;. [Epub ahead of print] PMID: 19117754
  18. Girvan AC, Teng Y, Casson LK, Thomas SD, Juliger S, Ball MW, Klein JB, Pierce WM Jr, Barve SS, Bates PJ.  AGRO100 inhibits activation of nuclear factor-{kappa}B (NF-{kappa}B) by forming a complex with NF-{kappa}B essential modulator (NEMO) and nucleolin.   Mol Cancer Ther. 5:1790-1799, 2006.
  19. Teng, Y, AC Girvan, LK Casson, SD Thomas, M Qian,  WM Pierce, Jr, and PJ Bates AS1411 Alters the Localization of a Complex Containing Protein Arginine Methyltransferase 5 and Nucleolin.  Cancer Res. Nov 1;67(21):10491-10500, 2007.

 

Selected Abstracts:

  1. Metry, Kristin J.1; Doll, Mark A.1; Smith, Ned B.1; Zhao, Shuang1; States, J. Christopher1; McGregor, W. Glenn1; Pierce, William M.1; Hein, David W.1  ROLE OF HUMAN N-ACETYLTRANSFERASE 2 ACETYLATION POLYMORPHISM IN MUTAGENESIS AND DNA ADDUCT FORMATION BY THE AROMATIC AMINE CARCINOGENS 2-AMINOFLUORENE AND 4-AMINOBIPHENYL  Society of Toxicology Annual Meeting, 2008
  2. Metry, Kristin J.1; Doll, Mark A.1; Smith, Ned B.1; Zhao, Shuang1; States, J. Christopher1; McGregor, W. Glenn1; Pierce, William M.1; Hein, David W.1  ROLE OF HUMAN N-ACETYLTRANSFERASE 2 ACETYLATION POLYMORPHISM IN MUTAGENESIS AND DNA ADDUCT FORMATION BY THE AROMATIC AMINE CARCINOGENS 2-AMINOFLUORENE AND 4-AMINOBIPHENYL  Society of Toxicology Annual Meeting, 2008
  3. Bendaly, Jean1; Zhao, Shuang1; Metry, Kristin J.1; Doll, Mark A.1; States, J. Christopher1; Smith, Ned B.1; Pierce, William M.1; Hein, David W.1 Role of human cytochrome P4501a1 and N-acetyltransferase 2 genetic polymorphism on the mutagenicity and DNA damage of the environmental carconogens 2-amino-phenylimidazo[4,5-B]pyridine and 4 aminoniphenyl.  Society of Toxicology Annual Meeting, 2008.
  4. David W. Hein, Kristin J. Metry, Jean Bendaly, Ned B. Smith, Jason R. Neale, and William M. Pierce, Jr. N-acetyltransferase 2 genotype-dependent DNA adducts in rapid and slow acetylator congenic rats administered 2-amino-3,8-dimethylimidazo-[4,5-f] quinoxaline  Presentation to the Symposium of the International Society for Polycyclic Aromatic Compounds., 2007.
  5. Voor, MJ; Xu, Q; Burden, RL Jr; Neale, J; Carter, H; Yang, S; Waddell, SW; Pierce, WM.  Alendronate is strongly anabolic in a young rat model of OVX-induced osteoporosis whereas hormones have little effect Transactions of the 53rd Annual Meeting of Orthopaedic Research Society, San Diego, California. 2007.
  6. Kennedy, MJ  Davis, DA  Smith, NB Pierce, WM  Lin, T  Pearce, RE Gaedigk, A  Kearns, GL Recombinant human growth hormone (rhGH) has no effect on activities of   hepatic cytochrome P4501A2 (CYP1A2), N-acetyltransferase 2 (NAT2),  xanthine oxidase (XO) or cytochrome P4502D6 in children with idiopathic  growth hormone (GH) deficiency.  Clin Pharm Therapeutics 79 (Suppl.): P84, 2006
  7. Kennedy, MJ  Smith, NB  Pierce, WM Lin, T Pearce, RE  Gaedigk, A  Sullivan, JE Chronic intermittent nocturnal hypoxemia alters activities of hepatic cytochrome P4501A2 (CYP1A2), N-acetyltransferase 2 (NAT2) and xanthine   oxidase (XO) in children with obstructive sleep APNEA (OSA). Clin Pharm Therapeutics 79 (Suppl.): P33, 2006
  8. Feldhoff, RC,    Bowen, KE  Cai, J  Pierce, WM  Houck, LD  Feldhoff, PW Disulfide bonding pattern in a structurally constrained family of 7 kDa vertebrate pheromones FASEB JOURNAL 20: (5 Pt.2) A972, 2006.
  9. Feng W, Cai J, Pierce WM, et al. Metallothionein transfers zinc to mitochondrial aconitase through a direct interaction and protects the protein from degradation FASEB JOURNAL 19 (5): A1108-A1108 Part 2 Suppl. S MAR 7 2005
  10. Merten KE, Feng W, Cai J, Pierce WM and Kang YJ  Cytochrome c oxidase Va, a possible protein involved in the metallothionein cardioprotection from oxidative injury FASEB JOURNAL 19 (5): A1108-A1108 Part 2 Suppl. S MAR 7 2005
  11. Barati, MT, Devillez, AB, Epstein, PN, Pierce, WM, McLeish, KR, Klein, JB Proteome map of the mouse renal glomeruli.  FASEB J. Mar 23, 2004 14:(Suppl S) A566, 2004
  12. Over-expression, purification, and characterization of CB2 cannabinoid receptor. R. Zhang, J Cai, W. M. Pierce and Z. H. Song, 10th SCBA International Symposium 2004

 

Patents & Licenses:

  1. W.M. Pierce, Jr.  Topical thiadiazole sulfonamide carbonic anhydrase inhibitors. U.S. Patent No. 5,242,937. Issued September 7, 1993.
  2. W.M. Pierce, Jr.  Topically active ocular benzothiazole sulfonamide carbonic anhydrase inhibitors.  U.S. Patent No. 5,055,480. Issued October 8, 1991.
  3. W.M. Pierce, Jr.  Topically active ocular gem-diacylthiadiazole sulfonamide carbonic anhydrase inhibitors.  U.S. Patent No. 5,059,613, Issued October 22, 1991.
  4. W.M. Pierce, Jr. and L.C. Waite.  Bone Targeted Carbonic Anhydrase Inhibitors.  U.S. Patent 5,641,762.  Issued June 24, 1997.
  5. WM Pierce, Jr., LC Waite and KG Taylor.  Bone Targeting Compounds for Delivering Agents to the Bone for Interaction Therewith.  U.S. Patent 7,196,220.  Issued March 27, 2007.