Eugene Mueller, Ph. D.

Charles L. Bloch Professor of Chemistry

Phone Number: 502-852-5811 (Office)


Department of Chemistry
University of Louisville
Louisville, KY 40292


Additional Appointments:

  • 1992 N.S.F. Postdoctoral Fellow, California Institute of Technology
  • 1994 N.I.H. Postdoctoral Fellow, Massachusetts Institute of Technology


  • 1987 B. S. Chemistry, University of Illinois–Urbana
  • 1992 Ph. D. Biochemistry, Harvard University


Laboratory Team Members:

Uyen Duong, Graduate Student
Austin Gibbs, Graduate Student
Kelly Sullivan, Ph. D., Postdoctoral Fellow
Miguel A. Blacutt, Undergraduate Student
Timothy Trey Justice, Undergraduate Student  
T.C. Klusman, Undergraduate Student
Shaunda L. Mitchell, Undergraduate Student 
Kenneth Pavy, Undergraduate Student
Brittany Sims, Undergraduate Student
James W. Rossman, Undergraduate Student
Kyle Vuong, Undergraduate Student                   
Russell Williams, Undergraduate Student
Tanner Brondhaver, Undergraduate Student (from Georgetown College)
Julia Springate, Undergraduate Student (from Centre College)
 Andrea Biscotti High School Student (duPont Manual) 


Research Focus:


tRNA Modification

Sulfur Transfer

Bioorganic Mechanism

Selected Publications:

  • Veerareddygari GR, Singh SK, Mueller EG. The Pseudouridine Synthases Proceed through a Glycal Intermediate. J Am Chem Soc. 2016 Jun 29;138(25):7852-5. PubMed PMID: 27292228; PubMed Central PMCID: PMC4929527.
  • Veerareddygari GR, Klusman TC, Mueller EG. Characterization of the catalytic disulfide bond in E. coli 4-thiouridine synthetase to elucidate its functional quaternary structure. Protein Sci. 2016 Jun 13;PubMed PMID: 27293139.
  • Miracco EJ, Bogdanov B, Mueller EG Unexpected linear ion trap collision-induced dissociation and Fourier transform ion cyclotron resonance infrared multi-photon dissociation fragmentation of a hydrated C-glycoside of 5-fluorouridine formed by the action of the pseudouridine synthases RluA and TruB. Rapid Commun Mass Spectrom. 2011 Sep 30;25(18):2627-32. PubMed PMID: 23657957.
  • Miracco EJ, Mueller EG The products of 5-fluorouridine by the action of the pseudouridine synthase TruB disfavor one mechanism and suggest another. J Am Chem Soc. 2011 Aug 10; 133(31): 11826-9. PubMed PMID: 21744792;PubMed Central PMCID: PMC3150545.
  • McDonald MK, Miracco EJ, Chen J, Xie, Y, Mueller EG. The handling of the mechanistic probe 5-fluorouridine by the pseudouridine synthase TruA and its consistency with the handling of the same probe by the pseudouridine synthases TruB and RluA. Biochemistry. 2011 Jan25;50(3):426-36. PubMed PMID: 21142053;PubMed Central PMCID: PMC3070203.
  • Mueller EG. Se-ing into selenocysteine biosynthesis. Nat Chem Biol. 2009 Sep;5(9):611-2. PubMed PMID: 19690534.
  • Crystal Structure of Pseudouridine Synthase RluA: Indirect Sequence Readout through Protein-Induced RNA Structure C. Hoang, J. Chen, C. A. Vizthum, J. M. Kandel, C. S. Hamilton, E. G. Mueller, and A. R. Ferré-d'Amaré Mol. Cell 2006, 24, 535-545. PubMed PMID: 17188032.
  • Mechanistic Investigations of the Pseudouridine Synthase RluA Using RNA Containing 5-Fluorouridine C. S. Hamilton, T. M. Greco, C. A. Vizthum, J. M. Ginter, M. V. Johnston, and E. G. Mueller Biochemistry 2006, 39, 12019-12038. PubMed PMID: 17002302;PubMed Central PMCID: PMC2580076.
  • Direct evidence for enzyme persulfide and disulfide intermediates during 4-thiouridine biosynthesis C. M. Wright, G. D. Christman, A. M. Snellinger, M. V. Johnston, and E. G. Mueller Chem. Comm. 2006, 3104-3106. PubMed PMID: 16855700.
  • Trafficking in persulfides: delivering sulfur in biosynthetic pathways E. G. Mueller Nature Chem. Biol. 2006, 4, 185-194. PubMed PMID: 16547


Recent Awards & Honors:

  • Organizing Committee for the XXIV tRNA Workshop in Olmué, Chile , 2012
  • Chair, Biochemistry Subcommittee for composition of the GRE subject test on Biochemistry Cellular and Molecular Biology
  • Advisory Committee American Chemical Society Biological Chemistry Division, 2012-2015