Dr. Harshini Sarojini

Tissue Ischemia and Energy Metabololism  laboratory

Our lab has showed that intracellular ATP delivery enhances diabetic wound healing by faster granulation tissue formation from 24hrs post injury. The goal of the proposed research is to investigate the series of mechanisms involved in this complex biological and molecular events in this fast granulation tissue formation. Specifically, we plan to discover the alternative pathway for macrophages to secrete collagen directly causing inflammatory cell-to extracellular matrix transformation resulting in extremely rapid tissue regeneration. I have the expertise, leadership and motivation necessary to successfully carry out the proposed work. 

I have a broad background in zoology, with specific training and expertise in biochemistry molecular biology research for this application.  As a postdoctoral fellow at Prof. Eugenia Wang’s lab in the University of Louisville, I carried out research on the role of miRNAs in the regulation of aging. My stay with Prof. Wang has been fairly productive yielding 12 publications. I expanded my research to include neurophysiological changes associated with aging. During my research work, I worked broadly on several techniques like; immunostaining, FISH, RNase protection assay, Northern/Southern Blotting, Colony hybridization, cloning of minigene and expression constructs, western blotting, Real Time PCR, PCR, Plasmid isolation, transfection, Genomic DNA and RNA isolations, Polyacrylamide & Agarose Gel Electrophoresis. I have been doing animal handling, breeding and isolating cortical neurons from the several mutant mice models. I have been trained to handle virus related work. I have been actively involved in generating lentiviral constructs to examine gain and loss of function of genes related to aging.