Bioanalytics

Photo of microscope in the pathology laboratoryThe core mission of the Bioanalytics Core is to provide investigators with technical expertise, education and mass-spectrometry based analytical services, and to develop new analytical approaches.

 

 

 


Aims of the Core

  1. Support current COBRE and Center projects. The Bioanalytics Core provides intellectual input, technical expertise and mass-spectrometry based analytical services, to address the needs of investigators.
  2. Educate and train investigators. The Core educates investigators about the principles of mass spectrometry, separations and other bioanalytical technologies. We work together to develop conceptual frameworks and execute well designed experiments.
  3. Develop new protocols and techniques. The Core utilizes mass spectrometry to design high sample and information throughput methods to accommodate the growing needs of researchers. Core personnel attend conferences and workshops to educate themselves about advancements in the field. The Core continues to expand its knowledge-base and technical expertise to satisfy the future needs of the Center.

Facilities & Instrumentation

The Mass Spectrometry laboratory is in the Delia Baxter II Biomedical Research Building (580 South Preston Street) on the University of Louisville Health Science Campus. It occupies over 2000 sq. ft of laboratory space and provides rich and diverse resources from which the investigators interface to develop their own independent research programs.

Instruments

 

Waters Synapt XS HRMS with ACQUITY UPLC I-Class System

Waters Synapt XS HRMS with
ACQUITY UPLC I-Class System

(obtained through the NIH Shared Instrumentation Grant Mechanism (S10 OD026840))
Agilent 6890N GC-MS with CTC PAL3 autosampler

Agilent 6890N GC-MS with
CTC PAL3 autosampler

 

Instrumentation shared with the Superfund Metabolism and Toxicity Core

 

Waters Xevo TQS-micro with ACQUITY UPLC H-Class System

Waters Xevo TQS-micro with
ACQUITY UPLC H-Class System
Agilent 6460 MS with 1290 UHPLC System and a multisampler

Agilent 6460 MS with
1290 UHPLC System and a multisampler

 

Waters Quattro Premier XE triple quadrupole MS with an Acquity Classic UPLC system

Waters Quattro Premier XE triple quadrupole MS
with an Acquity Classic UPLC system
Sciex5500 QTRAP MS with Shimadzu Nexera LC system

Sciex5500 QTRAP MS with
Shimadzu Nexera LC system

Core Services

Currently, our Core has excellent targeted analytical workflows based on the state-of-the art UPLC/UHLPC-MS/MS systems with triple-quadrupole analyzers and GC-MS. In August 2020 the Core added a high resolution, state-of-the art Waters Synapt XS LC-MS system (obtained through the NIH shared instrumentation grant mechanism – S10 OD026840).

The Bioanalytics Core facilitates characterization and quantitation of small molecular weight analytes in biofluids, tissues and cells.

Services available:

  • UPLC-MS/MS measurements
    • 28 VOC metabolites and 5 tobacco alkaloids in urine
    • Low abundance VOC metabolites in urine
    • Biogenic monoamines and their metabolites in urine
    • Histidine/histidine dipeptides in tissues/urine
    • Measurement of cortisol/cortisone in urine
    • Muconic acid in urine
    • LC-MS/MS analyses of 41 lipid mediators
  • Stable isotope resolved metabolomics analysis - data acquisition (Synapt XS) and analysis
    (The Core can analyze stable isotope labeled data from Waters and Thermo HRMS systems)
  • GC-MS*
    • Determination of short chain fatty acids in urine, feces and blood
    • Measurement of malondialdehyde in tissues and plasma
    • Amino acid assay – tissues and biofluids
  • Method development
    • Synapt XS untargeted and targeted method development
    • LC-MS/MS quantitative method development
    •  GC-MS method development
  • Training

* - minimum of 10 samples. Less samples will be charged minimum. For pricing inquiries and questions related to services offered, please contact Technical Director, Dr. Pawel Lorkiewicz.

Sample submission forms


Additional Areas of Expertise

  • Pawel Lorkiewicz, Ph.D. - GC-MS, LC-MS, FT-MS, HRAM, MALDI, GCxGC, SIRM, metabolomics, lipidomics, targeted analysis, surface analysis, tobacco products
  • Jason Hellman, Ph.D. - lipid mediator quantification, mass spectrometry, LC-MS, lipidomics
  • Shahid Baba, Ph.D. - dipeptide metabolism, LC-MS, targeted analysis, structural elucidation