Research Interests

Aruni Bhatnagar, Ph.D.

Professor of Medicine
Kanpur University, India (1986)

Research Interests

-- Environmental cardiology
-- Oxidative stress and cardiovascular function
-- Adverse effects of tobacco smoke on cardiovascular health
-- Toxicity of electronic cigarettes

Recent Publications

Haberzettl, P., McCracken, J., Bhatnagar, A. and Conklin, D. J.  (2016).  Insulin sensitizers prevent fine particulate matter-induced vascular insulin resistance and changes in endothelial progenitor cell homeostasis.  Am. J. Physiol. Heart Circ. Phys. 310:H1423-H1438.

Haberzettl, P., O’Toole, T.E., Bhatnagar, A. and Conklin, D. J.  (2016).  Exposure to fine particulate air pollution causes vascular insulin resistance by inducing pulmonary oxidative stress.  Environ. Health Perspect. 124:1830-1839.

Hall ME, Wang W, Okhomina V, Agarwal M, Hall JE, Dreisbach AW, Juncos LA, Winniford MD, Payne TJ, Robertson RM, Bhatnagar A, and Young BA.  (2016).  Cigarette Smoking and Chronic Kidney Disease in African Americans in the Jackson Heart Study.  J. Am. Heart Assoc. 5:pii:e003280.

Keith RJ, Al Rifai M, Carruba C, De Jarnett N, McEvoy JW, Bhatnagar A, Blaha MJ, and Defilippis AP.  (2016).  Tobacco Use, Insulin Resistance, and Risk of Type 2 Diabetes: Results from the Multi-Ethnic Study of Atherosclerosis.  PLoS One 11:e0157592.

Abplanalp WT, Conklin DJ, Cantor JM, Ginsberg MH, Wysoczynski M, Bhatnagar A, and O'Toole TE.  (2016).  Enhanced Integrin α4β1-Mediated Adhesion Contributes to a Mobilization Defect of Endothelial Progenitor Cells in Diabetes.  Diabetes 65:3505-3515.

Zhang, M.J., Sansbury, B.E., Hellmann, J., Baker, J.F., Guo, L., Parmer, C.M., Prenner, J.C., Conklin, D.J., Bhatnagar, A., Creager, M.A., and Spite, M.  (2016).  Resolvin D2 enhances post-ischemic revascularization while resolving inflammation.  Circulation 134:666-680.

Pope, CA 3rd, Bhatnagar, A., McCracken, J. P., Abplanalp, W., Conklin, D. J. and T. O'Toole.  (2016).  Exposure to fine particulate air pollution is associated with endothelial injury and systemic inflammation.  Circ. Res. 119:1204-1214.

Bhatnagar, A.  (2017).  Are electronic cigarette users at increased risk fo cardiovascular disease?  JAMA Cardiol. doi: 10.1001/jamacardio.2016.5550.

 

Daniel J. Conklin, Ph.D.

Professor of Medicine
University of Notre Dame (1995)

Research Interests

-- Environmental cardiology
-- Aldehydes and cardiovascular disease
-- Adverse effects of tobacco smoke on cardiovascular health
-- Toxicity of electronic cigarettes

Recent epidemiological studies reveal that cardiovascular disease and diabetes are antagonized by environmental pollutant exposure. The presence of aldehydes in complex air borne mixtures of particulate matter (e.g., smog, tobacco smoke, automobile exhaust, etc) is well-documented but the contribution these aldehydes play in causing or exacerbating cardiovascular disease is unknown.  We have focused on the action of acrolein, a particularly reactive aldehyde, in cardiovascular tissues.  Our studies show that acrolein is a powerful cardiovascular toxin that can induce dyslipidemia, block cardiac preconditioning, enhance thrombus formation, stimulate blood vessel hypercontraction and endothelial dysfunction, and elicit endothelial-dependent relaxation via NO- and EDHF-dependent pathways. Future studies will focus on uncovering the mechanisms of action of acrolein-induced cardiovascular toxicity for developing intervention strategies.

Recent Publications

Conklin,D.J., P. Haberzettl, G. Jagatheesan, S. Baba, M.L. Merchant, R.A. Prough, J.D. Williams, S.D. Prabhu andA. Bhatnagar. 2015. Glutathione S-transferase P protects against cyclophosphamide-induced cardiotoxicity in mice. Toxicol. Appl. Pharmacol. 285(2):136-48.

Conklin, D.J., Y. Guo, P. Haberzettl, B.G. Hill, S.P. Baba, L. Guo, G. Jagatheesan, K. Wetzelberger, D. Obal, D.G. Rokosh, R.A. Prough, S.D. Prabhu, M. Velayutham, J.L. Zweier, D. Hoetker, D.W. Riggs, S. Srivastava, R. Bolli, and A. Bhatnagar. 2015. Genetic deficiency of glutathione S-transferase P increases myocardial sensitivity to ischemia-reperfusion injury. Circ. Res. 117(5):437-49.

DeJarnett, N., R. Yeager, D.J. Conklin, J. Lee, T. O'Toole, J. McCracken, W. Abplanalp, S. Srivastava, D. Riggs, I. Hamzeh, S. Wagner, A. Chugh, A. DeFillipis, T. Ciszewski, B. Wyatt, C. Becher, D. Higdon, K. Ramos, D. Tollerud, J. Myers, S. Rai, S. Prabhu and A. Bhatnagar. 2015. Residential proximity to major roadways increases “early” circulating angiogenic cell levels. ATVB. 35(11):2468-77.

Conklin, D.J. 2016. Acute cardiopulmonary toxicity of inhaled aldehydes: role of TRPA1. Ann. N. Y. Acad. Sci. 1374(1):59-67.

Haberzettl, P., McCracken, J., Bhatnagar, A. and D.J. Conklin. 2016. Insulin sensitizers prevent fine particulate matter-induced vascular insulin resistance and changes in endothelial progenitor cell homeostasis. Am. J. Physiol. Heart Circ. Phys. 310(11):H1423-H1438.

Haberzettl, P., O’Toole, T.E., Bhatnagar, A. and D.J. Conklin. 2016. Exposure to fine particulate air pollution causes vascular insulin resistance by inducing pulmonary oxidative stress. Environ. Health Perspect. 124(12):1830-1839.

Zhang, M.J., Sansbury, B.E., Hellmann, J., Baker, J.F., Guo, L., Parmer, C.M., Prenner, J.C., Conklin, D.J., Bhatnagar, A., Creager, M.A., and M. Spite. 2016. Resolvin D2 enhances post-ischemic revascularization while resolving inflammation. Circulation 134(9):666-680.

Pope, CA 3rd, Bhatnagar, A., McCracken, J. P., Abplanalp, W., Conklin, D. J. and T. O'Toole. 2016. Exposure to fine particulate air pollution is associated with endothelial injury and systemic inflammation. Circ. Res. 119(11):1204-1214.

Conklin, D. J., Haberzettl, P., Jagatheesan, G., Kong, M., and Hoyle, G. W.  (2016).  Role of TRPA1 in acute cardioplumonary toxicity of inhaled acrolein.  Toxicol. Appl. Pharmacol. doi: 10.1016/j.taap.2016.08.028.

 

Gary Hoyle, PhD 

Professor and Acting Department Chair
Duke University (1988)

Research Interests

-- Pulmonary toxicology
-- Mechanisms of lung injury and repair
-- Chlorine toxicity
-- Development of treatments for lung injury

My research program focuses on lung injury and repair.  I have studied adverse health effects of a variety of environmental and occupational agents, including chlorine, ozone, isocyanates, asbestos, silica, bleomycin, allergens, cigarette smoke, ambient particulate matter, and acrolein.  My recent research has focused on understanding and developing treatments for lung injury caused by inhalation of chlorine gas.  Chlorine has a number of industrial uses, is produced in large quantities, is highly toxic, and is considered a chemical threat agent.  We have characterized injury and repair processes following chlorine inhalation and have tested therapies that are effective in treating both acute and persistent effects of chlorine on the respiratory tract.

Recent Publications

Chang, W., Chen, J., Schlueter, C. F., Rando, R. J., Pathak, Y. V., and Hoyle, G. W.  (2012).  Inhibition of chlorine-induced lung injury by the type 4 phosphodiesterase inhibitor rolipram.  Toxicol. Appl. Pharmacol. 263:251-258.

Musah, S., DeJarnett, N., and Hoyle, G. W.  (2012).  Tumor necrosis factor- mediates interactions between macrophages and epithelial cells underlying proinflammatory gene expression induced by particulate matter.  Toxicol. 299:125-132.

Musah, S., Chen, J., and Hoyle, G. W.  (2012).  Repair of tracheal epithelium by basal cells after chlorine-induced injury.  Respir. Res. 13:107.

Mo, Y., Chen, J., Schlueter, C. F., and Hoyle, G. W.  (2013).  Differential susceptibility of inbred mouse strains to chlorine-induced airway fibrosis.  Am. J. Physiol. Lung Cell. Mol. Physiol. 304:L92-L102.

Chen, J., Mo, Y., Schlueter, C. F., and Hoyle, G. W.  (2013).  Inhibition of chlorine-induced pulmonary inflammation and edema by mometasone and budesonide.  Toxicol. Appl. Pharmacol. 272:408-413.

Mo, Y., Chen, J., Humphrey, D. M. Jr., Fodah, R. A., Warawa, J. M., and Hoyle, G. W.  (2015).  Abnormal epithelial structure and chronic lung inflammation after repair of chlorine-induced airway injury.  Am. J. Physiol. Lung Cell. Mol. Physiol. 308:L168-L178.

Hoyle , G. W., Chen, J., Schlueter, C. F., Mo, Y., Humphrey, D. M. Jr., Rawson, G., Niño, J. A., and Carson, K. H.  (2016).  Development and assessment of countermeasure formulations for treatment of lung injury induced by chlorine inhalation.  Toxicol. Appl. Pharmacol. 298:9-18.

Musah, S., Schlueter, C. F., Humphrey, D. M. Jr., Powell, K. S., Roberts, A. M., and Hoyle G.W.  (2017).  Acute lung injury and persistent small airway disease in a rabbit model of chlorine inhalation.  Toxicol. Appl. Pharmacol. 315:1-11. 

Conklin, D. J., Haberzettl, P., Jagatheesan, G., Kong, M., and Hoyle, G. W.  (2016).  Role of TRPA1 in acute cardioplumonary toxicity of inhaled acrolein.  Toxicol. Appl. Pharmacol. doi: 10.1016/j.taap.2016.08.028.

 

Robert J. Jacobs, PhD

Professor; Director of MPH Program
University of North Carolina (1979)

Research Interests

-- Health effects of organic dusts
-- Environmental exposures assessment

Dr. Jacobs has published extensively on the health effects associated with specific components of inhaled organic dust (gram negative bacterial endotoxins) and on the development of methods for exposure assessment of specific biological airborne contaminants in both the work and non-work environments (gram negative bacterial endotoxins, mycotoxins, and beta 1,3 glucans).  He is past Chair of the Committee on Organic Dust of the International Commission for Occupational Health (ICOH) of the International Labor Organization (ILO) and has collaborated on international health projects in Sweden, Guyana, Surinam and Moldova.  Dr. Jacobs has also served as a member of local, regional, and national committees focused on assessing the impacts of climate change on public health.  In additional to his interest in environmental and occupational health, Dr. Jacobs has been involved in a diverse array of public health programs ranging from the development of vector control programs in developing countries to programs designed to enhance the training of the public health workforce.  In 2010-11 Dr. Jacobs was a Fulbright Scholar at the West China School of Public Health at Sichuan University in Chengdu, China.

Recent Publications

Chan C, Heinbokel JF, Myers JA, and Jacobs RR.  (2011)  Development and evaluation of a dynamic model that projects population biomarkers of methylmercury exposure from local fish consumption.  Integr. Environ. Assess. Manag. 7:624-635.

Chan C, Heinbokel JF, Myers JA, and Jacobs RR.  (2012)  A dynamic model using monitoring data and watershed characteristics to project fish tissue mercury concentrations in stream systems.  Environ. Assess. Manag. 8:709-722.

Chan C, and Jacobs RR.  (2013)  Analysis of potential mercury policies: the impact of stream basin characteristics on susceptible populations.  Integr. Environ. Assess. Manag. 9:124-133.

Jacobs, RR. (2016) Indoor Air and Public Health, Sustain (34): Spring/Summer.

Jacobs, RR. (2017) Endotoxin, In: Physical and Biological Hazards of the Workplace (3rd ed), Eds: Stave, GM and Wald. PH; Wiley.

 

Rachel Neal, PhD

Assistant Professor
University of Missouri - Rolla (1999)

Research Interests

-- Fetal basis of adult disease
-- Adverse health effects of maternal smoking
-- Health effects of gut microbiome

My research program utilizes high dimensionality data collection methodologies to probe the impact of adverse fetal environment on offspring health. We utilize metagenomic, proteomic, and metabolomic platforms to delineate the impact, in rodent models, of maternal licit drug use during fetal development on gut-liver-brain axis function.

Recent Publications

Lutz PM, Kelty EA, Brown TD, Wilson TJ, Brock G, Neal RE.  (2012).  Environmental cigarette smoke exposure modulates IgE levels of Pb-exposed children.  Toxicology 291:43-50.

Jagadapillai R, Chen J, Canales L, Birtles T, Pisano MM, Neal RE.  (2012).  Developmental cigarette smoke exposure: kidney proteome profile alterations in low birth weight pups.  Toxicology 299:80-89.

Canales L, Chen J, Kelty E, Musah S, Webb C, Pisano MM, Neal RE.  (2012).  Developmental cigarette smoke exposure: liver proteome profile alterations in low birth weight pups.  Toxicology 300:1-11.

Canales L, Gambrell C, Chen J, Neal RE.  (2013).  Prenatal alcohol exposure alters the cerebral cortex proteome in weanling rats.  Reprod. Toxicol. 39:69-75.

Neal RE, Chen J, Jagadapillai R, Jang H, Abomoelak B, Brock G, Greene RM, Pisano MM.  (2014).  Developmental cigarette smoke exposure: hippocampus proteome and metabolome profiles in low birth weight pups.  Toxicology 317:40-49.

Massey VL, Stocke KS, Schmidt RH, Tan M, Ajami N, Neal RE, Petrosino JF, Barve S, Arteel GE.  (2015).  Oligofructose protects against arsenic-induced liver injury in a model of environment/obesity interaction.  Toxicol. Appl. Pharmacol. 284:304-314.

Neal RE, Chen J, Webb C, Stocke K, Gambrell C, Greene RM, and Pisano MM.  (2016).  Developmental cigarette smoke exposure II: Hepatic proteome profiles in 6 month old adult offspring.  Reprod. Toxicol. 65:414-424.

Neal RE, Jagadapillai R, Chen J, Webb CL, Stocke K, Gambrell C, Greene RM, Pisano MM.  (2016).  Developmental cigarette smoke exposure II: Kidney proteome profile alterations in 6 month old adult offspring.  Reprod. Toxicol. 65:425-35.

Neal RE, Jagadapillai R, Chen J, Webb C, Stocke K, Greene RM, Pisano MM.  (2016).  Developmental cigarette smoke exposure II: Hippocampus proteome and metabolome profiles in adult offspring.  Reprod. Toxicol. 65:436-447.

 

J. Christopher States, Ph.D.

Professor of Pharmacology & Toxicology
Albany Medical College/Union University (1980)

Research Interests

-- Molecular biology and molecular genetics of DNA damage and repair in humans
-- Mechanisms of chemoresistance
-- Arsenic toxicity and cell cycle disruption

Recent Publications

Muenyi, CS, Pandit, A, Helm, CW, States, JC. Cisplatin Plus Sodium Arsenite And Hyperthermia Induces Pseudo-G1 Associated Apoptotic Cell Death In Ovarian Cancer Cells. Toxicological Sciences 139:74-82 (2014) PMID: 24519527

States, JC, Ouyang, M, Helm, CW. Systems Approach to Identify Environmental Exposures Contributing to Organ-Specific Carcinogenesis. Cancer Epidemiology 38:321-327 (2014) PMID: 24721284

Wahlang B, Falkner KC, Clair HB, Al-Eryani L, Prough RA, States JC, Coslo DM, Omiecinski CJ, Cave MC. Human receptor activation by aroclor 1260, a polychlorinated biphenyl mixture. Toxicological Sciences 140:283-297 (2014). PMID: 24812009

Wahlang B, Song M2 Beier JI, Cameron Falkner K, Al-Eryani L, Clair HB, Prough RA, Osborne TS, Malarkey DE, States JC, Cave MC. Evaluation of Aroclor 1260 exposure in a mouse model of diet-induced obesity and non-alcoholic fatty liver disease. Toxicol Appl Pharmacol. 279:380-90 (2014). PMID: 24998970

States JC. Disruption of Mitotic Progression by Arsenic. Biological Trace Elements in Research 166: 36-40 (2015) PMID: 25796515

Stepp MW, Mamaliga G, Doll MA, States JC, Hein DW. Folate-Dependent Hydrolysis of Acetyl-Coenzyme A by Recombinant Human and Rodent Arylamine N-Acetyltransferases. Biochem Biophys Rep. 3:45-50 (2015). PMID: 26309907

Muenyi CS, Ljungman M, States JC. Arsenic disruption of DNA damage responses – potential role in carcinogenesis and chemotherapy. Biomolecules 25:2184-93 (2015) PMID: 26404387

Wahlang B, Prough RA, Falkner KC, Hardesty JE, Song M, Clair HB, Clark BJ, States JC, Arteel GE, Cave MC. Polychlorinated Biphenyl-Xenobiotic Nuclear Receptor Interactions Regulate Energy Metabolism, Behavior, and Inflammation in Non-alcoholic-Steatohepatitis. Toxicol Sci. 149(2):396-410 (2016) PMID: 26612838

Carlisle SM, Trainor PJ, Yin X, Doll MA, Stepp MW, States JC, Zhang X, Hein DW. Untargeted polar metabolomics of transformed MDA-MB-231 breast cancer cells expressing varying levels of human arylamine N-acetyltransferase 1. Metabolomics 2016 Jul;12(7). pii: 111 (2016) PMID: 27872580


Qunwei, Zhang, MD, MPH, PhD

Professor
M.D., Fujian Medical University, P.R. of China (1989)
Ph.D., University of Fukui, Japan (2000)

Research Interests

-- Health effects of environmental/occupational agents
-- Toxicity and genotoxicity of nanoparticles

I have a long-term interest in studying health effects of various environmental and occupational agents including cigarette smoke, ambient particulate matter (PM), and various engineered nanoparticles.  Nanomaterials, which typically contain particles of ≤100 nm in diameter, can possess desirable physical, chemical, and optical properties, and they have multiple uses in electronics, manufacturing, cosmetics, and biomedical applications.  Novel nanomaterials may present unique safety hazards, and, despite their widespread use, the toxicity of nanoparticles is not well understood.  In my lab we have characterized the toxicity of nickel, cobalt and titanium dioxide nanoparticles, investigated mechanisms of toxicity, and sought ways to produce safer nanoparticles while still retaining desirable properties.

Recent Publications

Mo Y, Gu A, Tollerud DJ, Zhang Q. Nanoparticle toxicity and environmental impact. Oxidative stress and biomaterial. Editors: Thomas Dziubla and D. Allan Butterfield. Elsevier Ltd. (2016). ISBN: 978-0-12-803269-5, page 117-143.

Liu Q, Xu C, Ji G, Liu H, Mo Y, Tollerud DJ, Gu A, Zhang Q. Sublethal effects of zinc oxide nanoparticles on male reproductive cells. Toxicology in Vitro, 2016; 35: 131-138.  PMID: 27247145

Zhang Y, Mo Y, Gu A, Wan R, Zhang Q, Tollerud DJ. Effects of urban particulate matter with high glucose on human monocytes U937.  Journal of Applied Toxicology, 2016; 36: 586–595. PMID: 26179980

Yang X, Shao H, Liu W, Gu W, Shu X, Mo Y, Chen X, Zhang Q, Jiang M. Endoplasmic reticulum stress and oxidative stress are involved in ZnO nanoparticle-induced hepatotoxicity. Toxicology Letters, 2015; 234: 40-49. PMID: 25680694

Feng L, Zhang Y, Jiang M, Mo Y, Wan R, Jia Z, Tollerud DJ, Zhang X, Zhang Q.   Up-regulation of Gadd45a after exposure to metal nanoparticles: the role of hypoxia inducible factor 1a. Environmental Toxicology, 2015; 30 (4): 490-499. PMID: 24277352

Skeete Z, Cheng H, Crew E,  Shan S, Zhao W,  Cronk H,  Luo J, Zhang Q,  Zhong CJ.  Design of functional nanoparticles and assemblies for theranostic applications.  ACS Applied Materials & Interfaces, 2014; 6 (24): 21752-21768. PMID: 25111087

Mo Y, Wan R, Tollerud DJ, Zhang Q. Nanomaterials.  Cancer and Inflammation Mechanisms: Chemical, Biological, and Clinical Aspect. Editors: Yusuke Hiraku, Shosuke Kawanishi, Hiroshi Ohshima. John Wiley & Sons, Inc. (2014).  ISBN: 978-1-118-16030-5, page 235-249.

Wan R, Mo Y, Feng L, Chien S, Tollerud DJ, Zhang Q. DNA damage caused by metal nanoparticles: the involvement of oxidative stress and activation of ATM. Chemical Research in Toxicology, 2012; 25: 1402-1411. (Highlighting in this issue; 2012; 25(7):1283). PMID:22559321

Mo Y, Wan R, Chien S, Tollerud DJ, Zhang Q.  Combination effects of Cigarette smoke extract and ambient ultrafine particles on endothelial cells. Toxicology in Vitro, 2012; 26: 295-303. PMID: 22178768

Mo Y, Wan R, Zhang Q.  Application of reverse transcription-PCR and real-time PCR in nanotoxicity research. Nanotoxicity: Method and Protocols. In the series: Methods in Molecular Biology (Vol. 926). Editor: Reineke, Joshua. Springer Protocols (www.SpringerProtocols.com).  Humana Press (2012). Methods Mol. Biol. 2012; 926: 99-112.  PMID: 22975959

Wan R, Mo Y, Chien S, Li Y, Li Y, Tollerud DJ, and Zhang Q.  The role of hypoxia inducible factor-1α in MMP-2 and MMP-9 production by human monocytes exposed to nickel nanoparticles. Nanotoxicology, 2011; 5(4): 568-582. PMID: 21401309

Stay connected Youtube