Imaging and Physiology
The Imaging and Physiology Core promotes scientific excellence in investigations of cardiometabolic disease. It provides investigators with access to state-of-the-art instrumentation and expert instruction related to confocal imaging, assessment of cardiac function, and performance of surgery in mice.
The Core will promote education on the theory, as well as sound technical comprehension, related to the various instruments and approaches available. This Core enables the integration of biochemical insights at the level of intact cells, organs, and intact organisms.
Aims of the Core
- Support current COBRE and Center projects. The Director is dedicated to monitoring the specific needs of Center members as they may relate to confocal imaging, in vivo assessment of cardiac function, and surgical models.
- Educate and train investigators. Individuals with ongoing needs for specific pieces of instrumentation will not simply be consumers of the Core’s resources, rather they will be investigators versed in thoughtful application of a particular imaging or physiological modality.
- Develop new protocols and techniques. The Core has developed new confocal imaging approaches, new echocardiography techniques, and new mouse surgeries. All of these advances were brought to the Core to promote the investigations of the Center members, particularly the junior COBRE investigators.
Facilities & Instrumentation
Confocal Imaging Laboratory
The confocal imaging laboratory houses a state-of-the-art Nikon A1 laser scanning confocal microscope (photo, right). This system is mounted on a customized, inverted, fully electronic, TE-2000E2 microscope body.
This system offers unparalleled spatial resolution in the x, y, and z planes.
There are four excitation lines of 405, 488, 561, and 640nm, which are driven by four (4), independent solid-state lasers (superior stability and longevity) mounted on an acousto-optic tuning filter (AOTF)-integrated laser launch.
The Diabetes and Obesity Center houses also a VisualSonics Vevo 770 High Resolution in vivo Imaging System (photo, right). This system is a non-invasive ultrasound-based method for viewing extremely small physiological structures in mice.
The system images living tissue and blood flow with a spatial resolution down to 30 microns, the highest resolution available in real-time today.
Left Ventricular Catheterization System
The Core is also equipped with an ARIA-1 single-segment pressure-volume(P-V) conductance system (Millar Instruments, Houston, TX).
This system can simultaneously and continuously measure high-fidelity left ventricular pressure and volume in the intact beating hearts of small animals such as mice.
The UofL Imaging & Physiology Core offers several services for center members as well as other investigators and UofL faculty.