Imaging and Physiology

The Imaging and Physiology Core promotes scientific excellence in investigations of cardiometabolic disease. It provides investigators with access to state-of-the-art instrumentation and expert instruction related to confocal imaging, assessment of cardiac function, and performance of surgery in mice. The Core will promote education on the theory, as well as sound technical comprehension, related to the various instruments and approaches available. This Core enables the integration of biochemical insights at the level of intact cells, organs, and intact organisms.


  1. Support current COBRE and Center projects. The Director is dedicated to monitoring the specific needs of Center members as they may relate to confocal imaging, in vivo assessment of cardiac function, and surgical models.
  2. Educate and train investigators. Individuals with ongoing needs for specific pieces of instrumentation will not simply be consumers of the Core’s resources, rather they will be investigators versed in thoughtful application of a particular imaging or physiological modality.
  3. Develop new protocols and techniques. The Core has developed new confocal imaging approaches, new echocardiography techniques, and new mouse surgeries. All of these advances were brought to the Core to promote the investigations of the Center members, particularly the junior COBRE investigators.

Confocal Imaging Laboratory

Photo of confocal imaging laboratory

The confocal imaging laboratory houses a state-of-the-art Nikon A1 laser scanning confocal microscope (photo, right).  This system is mounted on a customized, inverted, fully electronic, TE-2000E2 microscope body.  This system offers unparalleled spatial resolution in the x, y, and z planes.  There are four excitation lines of 405, 488, 561, and 640nm, which are driven by four, independent solid-state lasers (superior stability and longevity) mounted on an acousto-optic tuning filter (AOTF)-integrated laser launch.

Photo of confocal imaging laboratory

The Diabetes and Obesity Center houses also a VisualSonics Vevo 770 High Resolution in vivo Imaging System (photo, right). This system is a non-invasive ultrasound-based method for viewing extremely small physiological structures in mice. The system images living tissue and blood flow with a spatial resolution down to 30 microns, the highest resolution available in real-time today.

Left Ventricular Catheterization System

The Core is also equipped with an ARIA-1 single-segment pressure-volume (P-V) conductance system (Millar Instruments, Houston, TX) that can simultaneously and continuously measure high-fidelity left ventricular pressure and volume in the intact beating hearts of small animals such as mice.

B.6.4.1. Confocal Imaging
a. Nikon A1 Confocal Use (standard, without Core Personnel)
i. $50/hr confocal time for Charter Members of the Center (one hour minimum)
b. Nikon A1 Confocal Use (standard, with Core Confocal Tech)
i. $75/hr confocal time for Charter Members of the Center (one hour minimum)
c. Nikon A1 Confocal Use (standard, with Core Director)
i. $100/hr confocal time for Charter Members of the Center (one hour minimum)
d. Nikon A1 Confocal Use (customized live-cell imaging, without Core Personnel)
i. $100/hr confocal time for Charter Members of the Center (one hour minimum)
e. Nikon A1 Confocal Use (customized live-cell imaging, with Core Confocal Tech)
i. $125/hr confocal time for Charter Members of the Center (one hour minimum)
f. Nikon A1 Confocal Use (customized live-cell imaging, with Core Director)
i. $150/hr confocal time for Charter Members of the Center (one hour minimum)

B.6.4.2. Cardiovascular Imaging (for mice only)
a. Left Ventricular Echocardiography on VisualSonics Vevo 770 System (non-invasive)
i. $20/echo for Charter Members of the Center
ii. Trained technician’s analysis of echocardiograms
- $10/echo for Charter Members of the Center
iii.  Core Director consultation regarding study results
- $75/hr for Charter Members of the Center (one hour minimum)
b. Arterial Catheterization for Left Ventricular Function (terminal procedure)
i. $100/mouse for Charter Members of the Center (including analysis)
ii. Core Director consultation regarding study results
- $75/hr for Charter Members of the Center (one hour minimum)
c. Blood Pressure Monitoring (Non-invasive tail cuff monitoring)
i. $10/mouse for Charter Members of the Center
ii. Core Director consultation regarding study results
- $50/hr for Charter Members of the Center (one hour minimum)

B.6.4.3.  Extracellular Flux Analysis (cultured cells or isolated tissue)
a. Performed by Core Personnel
i. $50/plate for Charter Members of the Center
b. Performed by Trained Investigator
i. $25/plate for Charter Members of the Center

B.6.4.4. Cardiovascular Surgery (for mice only)
a. Non-reperfused, Myocardial Infarction OR Transverse Aortic Constriction
i. $30/mouse for Charter Members of the Center
b. Myocardial Ischemia-Reperfusion Surgery in Mice
i. $100/mouse for Charter Members of the Center (includes infarct size analysis by Core)
c. Miscellaneous Procedures
Rates for any other procedures needed by investigators will be negotiated prior to the performance of any work.  The Core is skilled at and approved for hind-limb ischemia, renal ischemia-reperfusion injury, and liver ischemia-reperfusion injury.  Because there is little to no ongoing need for these procedures, costs will be determined for these (or any other) procedures, as they arrive.  The point of emphasis here is the Core’s willingness and flexibility to adopt whatever technique/protocol is necessary for the investigators it serves.


Contact

Email Steven Jones
580 S. Preston St.
Baxter II, Room  321
Louisville, KY 40202


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