Biomimetic Membranes and Structures Lab

Location
Ernst Hall Room 207
Core Scientific and Technical Staff
PI:  Joel R. Fried

Joel Fried Group Image

Research Overview

The Biomimetic Membrane and Structures group utilizes a two-pronged approach to study the use of block-copolymers as a support structure for various natural and synthetic membrane bound molecules which are applicable in water purification, ionic sensors and targeted drug delivery. Our group strives to utilize both computational and experimental techniques to gain insight on biological/biomimetic systems. From an experimental standpoint, the activity of membrane bound ion channels are studied electrically using a technique called planar bilayer lipid membrane (BLM). Additionally, we use molecular dynamics(MD) simulations to gain further insight on how parameter such as membrane hydrophobicity, thickness, flexibility, and lateral pressure effect the activity of membrane bound molecules. MD simulations are also used to investigate the self-assembly process of biomimetic polymer systems.  Lastly, we constantly seek out internal and external research collaborations. Currently, our group is working in collaboration with the Department of Surgery at the University of Louisville Medical School while our external collaborations include both academic and industrial groups.

Equipment

Experimental

  • Planar Bilayer Lipid Membrane(BLM) station with data acquisition system.
  • Leica inverted microscope

Computational

EXXACT  Workstation

  • Intel Xeon CPU E5-2640 v4, dual 10-Core Processor@ 2.40GHz. GPU: 2 Nvidia Tesla K80

Dell Workstations

  • 2 Dell Precision Towers 7910: Xeon E5-2630 v3, dual 8-Core Processor @ 2.4 GHz
  • 2 Dell Precision Towers 7810XL: Xeon E5-2687W v4, dual 12-Core Processor @ 3.0 GHz

Ohio supercomputer center

  • OWENS Cluster: 160 GPU computation nodes with Dell PowerEdge R730 two-socket servers with Intel Xeon E5-2680 v4 (14 cores, 2.40GHz) and a Nvidia Tesla P100 GPU per node

 

Projects

Experimental

  • Exploration of synthetic ion channels and biomimetic polymer membranes in combination with natural lipid membranes and ion channels, respectively.
  • Design of new membrane support apparatus for planar BLM station.

Computational

  • Parameterization and validation of MD forcefield (capable of simulations times > 1 μs) for biomimetic membrane systems.
  • Simulating reconstitution and transport mechanisms of a synthetic ion channel in a lipid membrane.

Scan Image

Contact Info

For additional information regarding this research group please contact Joel R. Fried via email. joel.fried@louisville.edu