UofL Genomics Facility Objectives and Resources:

The UofL Genomics Facility is located in the Clinical Translational Research Building Labs 227 F-H on the UofL Health Science Center Campus. It has been operational since Spring 2001 under the direction of Dr. Wolfgang Zacharias. The facility houses two Illumina Next Generation Sequencing platforms and a 10X Chromium single-cell-Seq instrument system. It is staffed with a full-time Facility Manager (Sabine Waigel) and three Sr. Research Technologists (Mei Zhang, Vennila Arumugam; Ashley Wise-Mitchell).

The team provides support for project design, logistics and management, performs platform-specific sample processing and instrument protocols as well as data collection, analysis and storage. Services offered include microarray-based global RNA or microRNA expression analyses, SNP profiling, NextGen genomic sequencing methods, RNA, microRNA, and metagenomics profiling, and targeted amplicon sequencing. As novel state-of-the-art technology, the Facility performs single-cell sequencing and cluster identifications on heterogeneous cell populations. The Facility also performs nucleic acid quality/quantity tests, assists in quantitative real-time PCR assays, and offers guidance in data analysis and mining.

The Objectives of the Facility are:

1. To provide high-quality services for NextGenSeq and single-cell-Seq analysis for UofL users;

2. To continuously incorporate the newest genomics protocols and analysis tools into the menu of services provided;

3. To educate and train users in applications, strengths, and limitations of novel genomics technologies;

4. To assist users in experimental design, sample processing, and data analysis;

5. To maintain close intellectual and logistic interactions with the KBRIN Bioinformatics Core to support customer needs in experimental design, data analysis and interpretation;

6. To provide logistic support for grant applications and manuscript preparations applying genomics technologies.

Instrumentation in the Facility include two Illumna NextGenSeq platforms with different capacities (MiSeq, NextSeq). In addition, an Applied Biosystems ViiA™ 7 real-time PCR System, a Covaris S220 Ultrasonicator, a Sage Science Pippin Prep size fractionator, an Agilent Bioanalyzer 2100, a Nanodrop One, a Qubit fluorimeter, a Countess II FL live/dead cell counter, a Miltenyi GentleMACS™ Octo Tissue Dissociator, and three Applied Biosystems PCR Thermocyclers 9700 are available. The Facility has licenses for the latest versions of the following data analysis software packages; all of them can be accessed by the user community: Partek Genomics Suite (Partek, Inc.); MetaCore & MetaDrug (Thomson Reuters, Inc.).

The UofL Genomics Facility has regular interactions with other Core Facilities and Research Centers on Campus. Also, the close relationship with the Tissue Biorepository in the J.G. Brown Cancer Center enables access to human tissue samples with known pathologic characteristics and appropriate control specimens. The Facility continues to regularly organize and hold workshops on campus to train and update researchers in various genomic techniques and different data analysis applications.

The Facility also continues to manage the Campus-wide Life Technologies Supply Center. Reagent and supply items from Life Technologies, Invitrogen, ABI, Ambion, Gibco and Molecular Probes are ordered for a low flat rate shipping charge to be delivered to our Supply Center for individual investigators; popular items are stocked. The Facility Director Dr. Zacharias and Manager Sabine Waigel are members of the UofL Institutional Core Review Committee, with the mission to re-organize, define, and provide guidelines for classification and management of UofL Core Facilities.


Lab Manager: