Laboratory Fume Hood Testing Program

Fume hoods, more properly referred to as chemical hoods, are often the primary control device when using flammable and toxic chemicals in the laboratory. They are intended to contain contaminants within the hood and exhaust the contaminated air outdoors.

Testing of Chemical Hood Exhaust

Prior to occupancy of newly constructed research facilities, a Test and Balance contractor ensures that chemical hoods are functioning according to design specifications. After occupancy, DEHS tests the airflow of chemical hoods at least annually. If deficiencies are found the information is forwarded to Physical Plant to initiate repairs. The yellow hood certification label affixed on the left side of the hood indicates the most recent test date of the hood. If your hood has not been retested by the expiration date, contact the Lab Safety Coordinator .

Daily Monitoring of Hood Exhaust

Chemical hoods should be equipped with an air flow monitor/alarm. Older hoods may not have a monitor/alarm and should have, at a minimum, a plastic "flow check ribbon" installed on the sash. When there is proper exhaust flow the ribbon is drawn gently inside the hood. Be alert to the monitor or ribbon. If there is a problem with the hood, contact the Physical Plant Work Control office for repair. If your hood does not have a flow monitor or flow check ribbon, contact the Lab Safety Coordinator .

Biological Safety Cabinets

Chemical hoods should be differentiated from Biological Safety Cabinets (BSC's), "culture hoods" and clean air benches, which have a HEPA-filtered, laminar flow supply component that protects specimens/products inside the cabinet from contaminants in the laboratory ambient air. BSC's may also exhaust contaminants outside the building, but more commonly recirculate HEPA-filtered exhaust air back into the lab. For this reason recirculating BSC's do not provide personnel protection from flammable and toxic gases and vapors. For more information on certification requirements and proper use of BSC's see Biological Safety.

Report a Malfunctioning Chemical Hood Chemical Hood User's Guide
Safe Operation Procedures for Chemical Hoods (PDF)

Department of Environmental Health And Safety

Main Office

1800 Arthur Street

Louisville, Kentucky 40208


tel (502) 852-6670

fax (502) 852-0880

Department of Environmental Health And Safety

Radiation Safety Office

319 Abraham Flexner Way

Louisville, Kentucky 40202


tel (502) 852-5231

fax (502) 852-8911

Office Hours

M-F 8:00 am to 4:30 pm

No holiday hours

IH Service Account

IBC Service Account