Eugene G. Mueller
Charles L. Bloch Professor of Chemistry Department of Chemistry University of Louisville Louisville, KY 40292(502) 852-5811 (phone)
(502) 852-7214 (fax)
office: 346 BRB
Education
Ph.D. in Biochemistry, 1992
Harvard University
Advisor: Jeremy R. Knowles
Mechanistic Enzymology: Studies on the Relative Stabilities of Enzyme-Bound States and on the Stereochemistry of Phospho Group Tranfers
B.S. in Chemistry, 1987
Summa Cum Laude with Highest Distinction in the Curriculum
University of Illinois at Urbana-Champaign
Awards and Honors
National Science Foundation Postdoctoral Fellow in Chemistry
National Science Foundation Predoctoral Fellow
Phi Beta Kappa
Bronze Tablet (University Honors of the U. of Illinois)
Marvel Prize for undergraduate research
Alumni Award in Chemistry
Psi Lambda Upsilon
Previous Employment
Department of Chemistry and Biochemistry
University of Delaware
Associate Professor, 2001-2007
Assistant Professor, 1995-2001
Professional Associations
The RNA Society, 1997-
American Society for Biochemistry & Molecular Biology, 1996-
Society of Biological Inorganic Chemistry, 1996-
American Association for the Advancement of Science, 1993-
American Chemical Society, 1987-
Publications
Crystal Structure of Pseudouridine Synthase RluA: Indirect Sequence Readout through Protein-Induced RNA Structure
Charmaine Hoang, Junjun Chen, Caroline A. Vizthum, Jason M. Kandel, Christopher S. Hamilton, Eugene G. Mueller, and Adrian Ferré-d'Amaré
Mol. Cell 2006, 24, 535-545.
DOI: 10.1016/j.molcel.2006.09.017
Mechanistic Investigations of the Pseudouridine Synthase RluA Using RNA Containing 5-Fluorouridine
Christopher S. Hamilton, Todd M. Greco, Caroline A. Vizthum, Joy M. Ginter, Murray V. Johnston, and Eugene G. Mueller
Biochemistry 2006, 45, 12029-12038.
DOI: 10.1021/bi061293x
Direct evidence for enzyme persulfide and disulfide intermediates during 4-thiouridine biosynthesis
Chapman M. Wright, Glenn D. Christman, Ann M. Snellinger, Murray V. Johnston, and Eugene G. Mueller
Chem. Comm. 2006, 3104-3106.
DOI: 10.1039/b604040c
Trafficking in persulfides: delivering sulfur in biosynthetic pathways
Eugene G. Mueller
Nature Chem. Biol. 2006, 2, 185-194.
DOI: 10.1038/nchembio779
March 26, 2006 Nature podcast interview of Prof. Mueller
Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain
Charmaine Hoang, Christopher S. Hamilton, Eugene G. Mueller, and Adrian Ferré-d'Amaré
Prot. Sci. 2005, 14, 2201-2206.
DOI: 10.1110/ps.051493605
The roles of the essential Asp-48 and highly conserved His-43 elucidated by the pH dependence of the pseudouridine synthase TruB
Christopher S. Hamilton, Christopher J. Spedaliere, Joy M. Ginter, Murray V. Johnston, and Eugene G. Mueller
Arch. Biochem. Biophys. 2005, 433, 322-334.
DOI: 10.1016/j.abb.2004.09.009
The pseudouridine synthases: revisiting a mechanism that seemed settled
Christopher J. Spedaliere, Joy M. Ginter, Murray V. Johnston, and Eugene G. Mueller
J. Am. Chem. Soc. 2004, 126, 12758-12759.
DOI: 10.1021/ja046375s.
Not all pseudouridine synthases are potently inhibited by RNA containing 5-fluorouridine
Christopher J. Spedaliere and Eugene G. Mueller
RNA 2004, 192-199.
DOI: 10.1261/rna.5100104
A paradigm for biological sulfur transfers via persulfide groups: a persulfide–disulfide–thiol cycle in 4-thiouridine biosynthesis
Chapman M. Wright, Peter M. Palenchar, and Eugene G. Mueller
Chem. Comm. 2002, 2708-2709.
DOI: 10.1039/b208626c
Chips off the old block
Eugene G. Mueller
Nature Structural Biology 2002, 9, 320-322.
The Role of the Cysteine Residues of ThiI in the Generation of 4-Thiouridine in tRNA
Eugene G. Mueller, Peter M. Palenchar, and Christopher J. Buck
Journal of Biological Chemistry 2001, 276, 33588-33595.
Will Introduction of an E. Coli Gene Lead to 4-Thiouridine in yeast tRNA?
Jaimie S. Robinson and Eugene G. Mueller
FASEB Journal 2001, 15, A188.
Functional Importance of Motif I of Pseudouridine Synthaes: Mutagenesis of Aligned Lysine and Proline Residues
Christopher J. Spedaliere, Christopher S. Hamilton, and Eugene G. Mueller
Biochemistry 2000, 39, 9459-9465.
Evidence that ThiI, an Enzyme Shared between Thiamin and 4-Thiouridine Biosynthesis, May Be a Sulfurtransferase that Proceeds through a Persulfide Intermediate
Peter M. Palenchar, Christopher J. Buck, Hui Cheng, Timothy J. Larson, and Eugene G. Mueller
Journal of Biological Chemistry 2000, 275, 8283-9296.
Role of Cysteine Residues in Pseudouridine Synthases of Different Families
Vidhyashankar Ramamurthy, Steven L. Swann, Christopher J. Spedaliere, and Eugene G. Mueller
Biochemistry 1999, 38, 13106-13111.
Using Genomic Information to Investigate the Function of ThiI, an Enzyme Shared between Thiamin and 4-Thiouridine Biosynthesis
Eugene G. Mueller and Peter M. Palenchar
Protein Science 1999, 8, 2424-2427.
Critical Aspartic Acid Residues in Pseudouridine Synthases
Vidhyashankar Ramamurthy, Steven L. Swann, Jennifer L. Paulson, Christopher J. Spedaliere, and Eugene G. Mueller
Journal of Biological Chemistry 1999, 274, 22225-22230.
Identification of a Gene Involved in 4-Thiouridine Generation in tRNA
Mueller, Eugene G., Buck, Christopher J., Palenchar, Peter M., Paulson, Jennifer L., and Barnhart, Lora J.
Nucleic Acids Research 1998, 26, 2606-2610.
Purple Acid Phosphatase: A Diiron Enzyme that Catalyzes a Direct Phospho Group Transfer to Water
Mueller, Eugene G., Crowder, Michael W., Averill, Bruce A., and Knowles, Jeremy R.
Journal of the American Chemical Society 1993, 115, 2974-2975.
Purple Acid Phosphatase Catalyzes the Direct Transfer of a Phospho Group from Substrate to Water
Mueller, Eugene G., Crowder, Michael W., Averill, Bruce A., and Knowles, Jeremy R.
FASEB Journal 1993, 7, A1065.
Stereochemical Course of the Reactions Catalyzed by the Bacterial Phosphoenolpyruvate:Mannitol Phosphotransferase System
Mueller, Eugene G., Khandekar, Sanjay, S., Knowles, Jeremy R.and Jacobson, Gary R.
Biochemistry 1990, 29, 6892-6896.