Cell Sorting

Brown Cancer Center Flow Cytometry Facility

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  • Two MoFlo cell sorters (Beckman Coulter)
  • Both machines are equipped with two lasers – Blue (488 nm) and Red (635 nm)
  • Access to a trained MoFlo operator who is experienced in various imaging techniques and is ready to provide advice on experimental design
  • The facility is available to all Brown Cancer Center members, University researchers, and affiliated investigators.
  • Director:
  • Mariusz Z. Ratajczak, PhD
  • Contact:
  • Chris Worth, Core Manager, Laboratory: 502-852-7521; Office: 502-852-7191, Email
  • Location:
  • Room SB16, Developmental Biology Institute Research Building, 501 South Floyd Street, Louisville, KY

Diabetes and Obesity Center

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  • One MoFlo cell sorters (Beckman Coulter)
  • Becton Dickinson LSR II
  • Becton Dickinson FASCalibur
  • Acuri C6 flow cytometer
  • Director:
  • Tim O'Toole, PhD
  • Contact:
  • James McCracken, PhD, Email, 502-852-4209
  • Location:
  • MoFlo: MDR Building
    Becton Dickinson LSR II: Second floor of the Baxter II Building
    Becton Dickinson FASCalibur:

Center for Predictive Medicine - Immunology Core Facility

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  • Becton Dickinson LSRFortessa analyzer:  (3 lasers-Blue 488nm, Red 640nm and Violet 405nm)
  • Becton Dickinson FACSAria III cell sorter: (2 lasers-Blue 488nm and Red 635nm)
  • Option once trained to self-operate the BD LSRFortessa
  • Cell Sorting using the BD FACSAria III will be performed by the Flow Cytometry staff
  • Trained support staff available for training and experimental design.
  • The Microbiology and Immunology Flow Cytometry Core Facility is available to all University investigators as well as private concerns.
  • Contact:
  • Email CPM, Main Office Number: 502-852-1339, Fax Number: 502-852-5468
  • Location:
  • CTR Building, 505 S. Hancock St., Sixth Floor, Louisville, KY

Microbiology and Immunology Flow Cytometry Core Facility

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  • Becton Dickinson LSRFortessa analyzer:  (3 lasers-Blue 488nm, Red 640nm and Violet 405nm)
  • Becton Dickinson FACSAria III cell sorter: (2 lasers-Blue 488nm and Red 635nm)
  • Option once trained to self-operate the BD LSRFortessa
  • Cell Sorting using the BD FACSAria III will be performed by the Flow Cytometry staff
  • Trained support staff available for training and experimental design.
  • The Microbiology and Immunology Flow Cytometry Core Facility is available to all University investigators as well as private concerns.
  • Director:
  • Bob Miller, PhD
  • Contact:
  • Bob Miller, Email Phone: 502-852-6273; Laboratory: 502-852-8912
  • Location:
  • CTR Building Room 651, 505 South Hancock Street, Louisville, KY, 40202