Genomics Core

UofL Genomics Facility

The UofL Genomics Facility provides gene expression, genotyping and DNA sequencing services. We also assist with real-time PCR and quantitative as well as qualitative nucleic acid analysis.

The UofL Genomics Facility is soliciting proposals for Next-Generation Sequencing Projects.  Awards will be in the form of vouchers that cover the cost of sequence library construction, sequencing runs and primary bioinformatics analysis.  All other costs are the responsibility of the researcher.  Approximately five to six vouchers will be awarded.  Final support commitments will be made pending availability of funds for the KBRIN P20GM103436 cycle in Spring 2021 Deadline is December 18, 2020. Click here for application information.

Next-Generation Sequencing Services

      • Illumina MiSeq
      • Illumina NextSeq
      • Contact: Sabine Waigel,Email Sabine
      • Location: CTR Building, Room 227 F-H, 505 S. Hancock St, Louisville, KY

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Single-Cell Sequencing Services

      • 10X Chromium Controller
      • Contact: Sabine Waigel, Email Sabine
      • Location: CTR Building, Room 227 F-H, 505 S. Hancock St, Louisville, KY

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qPCR

      • Real-time quantitativePCR (ViiA7: 96-well FAST, 384-well, and Array Card block)
      • Contact: Sabine Waigel, Email Sabine
      • Location: CTR Building, Room 227 F-H, 505 S. Hancock St, Louisville, KY

Request Services

Other Services

      • RNA analysis: We offer access to the Nanodrop One and ABI Qubit for quantitative nucleic acid measurements and to Agilent’s BioAnalyzer 2100 for RNA quality assessment.
      • PCR: The Facility has an ABI ViiA-7 real-time PCR system, which can be used by our customers on request.
      • Ultrasonication: Our Covaris S220 focused ultrasonicator performs DNA, RNA and chromatin shering, tissue homogenization, cell lysis, etc. It is used only by Core staff but is available for customer samples.
      • Training: On request, we offer training in many areas of genomic research, including real-time PCR, Nanodrop, BioAnalyzer, RNA isolation, sample preparation for microarrays and NextGenSeq library preparations.
      • Data Mining: We offer free access to the latest versions of Metacore and Partek and will be able to assist with the use of either software.

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