Genomics Core
UofL Genomics Facility
The UofL Genomics Facility provides gene expression, genotyping and DNA sequencing services. We also assist with real-time PCR and quantitative as well as qualitative nucleic acid analysis.
Next Generation Sequencing Service
- Illumina MiSeq
- Illumina NextSeq
- Contact: Sabine Waigel,Email Sabine
- Location: CTR Building, Room 227 F-H, 505 S. Hancock St, Louisville, KY
Single-Cell Sequencing Services
- 10X Chromium Controller
- Contact: Sabine Waigel, Email Sabine
- Location: CTR Building, Room 227 F-H, 505 S. Hancock St, Louisville, KY
qPCR
- Real-time quantitativePCR (ViiA7: 96-well FAST, 384-well, and Array Card block)
- Contact: Sabine Waigel, Email Sabine
- Location: CTR Building, Room 227 F-H, 505 S. Hancock St, Louisville, KY
Other Services
- RNA analysis: We offer access to the Nanodrop One and ABI Qubit for quantitative nucleic acid measurements and to Agilent’s BioAnalyzer 2100 for RNA quality assessment.
- PCR: The Facility has an ABI ViiA-7 real-time PCR system, which can be used by our customers on request.
- Ultrasonication: Our Covaris S220 focused ultrasonicator performs DNA, RNA and chromatin shering, tissue homogenization, cell lysis, etc. It is used only by Core staff but is available for customer samples.
- Training: On request, we offer training in many areas of genomic research, including real-time PCR, Nanodrop, BioAnalyzer, RNA isolation, sample preparation for microarrays and NextGenSeq library preparations.
- Data Mining: We offer free access to the latest versions of Metacore and Partek and will be able to assist with the use of either software.