Genomics Core

UofL Genomics Facility

The UofL Genomics Facility provides gene expression, genotyping and DNA sequencing services. We also assist with real-time PCR and quantitative as well as qualitative nucleic acid analysis.

Next Generation Sequencing Service

      • Illumina MiSeq
      • Illumina NextSeq
      • Contact: Sabine Waigel,Email Sabine
      • Location: CTR Building, Room 227 F-H, 505 S. Hancock St, Louisville, KY

Request Services

Single-Cell Sequencing Services

      • 10X Chromium Controller
      • Contact: Sabine Waigel, Email Sabine
      • Location: CTR Building, Room 227 F-H, 505 S. Hancock St, Louisville, KY

Request Services

qPCR

      • Real-time quantitativePCR (ViiA7: 96-well FAST, 384-well, and Array Card block)
      • Contact: Sabine Waigel, Email Sabine
      • Location: CTR Building, Room 227 F-H, 505 S. Hancock St, Louisville, KY

Request Services

Other Services

      • RNA analysis: We offer access to the Nanodrop One and ABI Qubit for quantitative nucleic acid measurements and to Agilent’s BioAnalyzer 2100 for RNA quality assessment.
      • PCR: The Facility has an ABI ViiA-7 real-time PCR system, which can be used by our customers on request.
      • Ultrasonication: Our Covaris S220 focused ultrasonicator performs DNA, RNA and chromatin shering, tissue homogenization, cell lysis, etc. It is used only by Core staff but is available for customer samples.
      • Training: On request, we offer training in many areas of genomic research, including real-time PCR, Nanodrop, BioAnalyzer, RNA isolation, sample preparation for microarrays and NextGenSeq library preparations.
      • Data Mining: We offer free access to the latest versions of Metacore and Partek and will be able to assist with the use of either software.

Request Services