Scott et al, Jan 2006, Interleukin-10 suppresses natural killer cell but not natural killer T cell activation during bacterial infection
Reference
Scott, M. J.,
Hoth, J. J.,
Turina, M.,
Woods, D. R.,
&
Cheadle, W. G.
Interleukin-10 suppresses natural killer cell but not natural killer T cell activation during bacterial infection. Cytokine, 33(2): 79-86. (Professional Student Author(s): Turina,M.) (2006).
Abstract
Interleukin (IL)-10 is an anti-inflammatory cytokine known to modulate the outcome of sepsis by decreasing pro-inflammatory cytokine production, including IL-12. a main activator of natural killer (NK) cells. We hypothesized that neutralization of IL-10 would increase NK and natural killer T (NKT) cell activation through increased IL-12 in a Mouse model of bacterial peritonitis. NK and NKT cell activations were measured by CD69 expression on NK 1.1+/CD3- and NK1.1+/CD3+ cells after cecal ligation and puncture (CLP). NK cells were significantly more activated in mice treated with anti-IL-10 antibodies, whereas no such effect was observed in NKT cells. Similarly, intracellular interferon gamma (IFN-gamma) levels were increased in NK cells of anti-IL-10-treated mice, but not in NKT cells. IL-12 and IL-18 levels were increased in both CLP groups, but in anti-IL-10-treated mice, early IL-12 and late IL-18 levels were significantly higher than in controls. Survival at 18h after CLP was lower in anti-IL-10 mice, which was associated with increased liver neutrophil accumulation. In summary, these data show an activating effect of IL-10 on NK, but not on NKT cells after CLR which corresponded with decreased Survival, higher IFN-gamma production, and increased remote organ neutrophil accumulation. These effects were not mediated by IL-12 and IL-18 alone, and reinforce a role for NK cells in remote organ dysfunction following peritonitis. (c) 2006 Elsevier Ltd. All rights reservedKeywords
- 59
- Antibodies
- BACTERIAL PERITONITIS
- CD69
- Cell
- CELLS
- CLP
- CYTOKINE
- CYTOKINE PRODUCTION
- DYSFUNCTION
- expression
- IL-12
- INFECTION
- INFLAMMATORY RESPONSE
- interferon gamma
- INTERFERON-GAMMA PRODUCTION
- Interleukin-10
- Ligation
- Liver
- lymphocyte
- ME
- Mice
- MODEL
- MORTALITY
- Multiple Organ Failure
- neutrophil
- NK CELLS
- organ dysfunction
- Peritonitis
- ref-journal
- Sepsis
- SEPTIC PERITONITIS
- SURVIVAL
