Skip to content. | Skip to navigation

Advanced Search…
Personal tools
 
You are here: Home → For Faculty & Staff → Reference Search → 2002 References → Yolcu et al, Dec 2002, Cell membrane modification for rapid display of proteins as a novel means of immunomodulation: FasL-decorated cells prevent islet graft rejection

Yolcu et al, Dec 2002, Cell membrane modification for rapid display of proteins as a novel means of immunomodulation: FasL-decorated cells prevent islet graft rejection

Reference

Yolcu, E. S., Askenasy, N., Singh, N. P., Cherradi, S. E., & Shirwan, H.  Cell membrane modification for rapid display of proteins as a novel means of immunomodulation: FasL-decorated cells prevent islet graft rejection. Immunity., 17(6): 795-808. (Professional Student Author(s): Yolcu, E.S.; Singh, N.P.; Cherradi, S.E.) (2002).

Abstract

Long-term display of exogenous proteins on the cell surface may have important research and therapeutic implications. We report a novel method for the cell-surface display of proteins that involves generation of a chimeric protein with core streptavidin, biotinylation of cells, and "decoration" with the protein. A chimeric protein with the extracellular portions of FasL (SA-FasL) was efficiently displayed on the cell surface within 2 hr without detectable cellular toxicity. Biotin and SA-FasL persisted on the cell surface for weeks in vitro and in vivo. Immunomodulation with SA-FasL-decorated splenocytes effectively blocked alloreactive responses in naive and presensitized rodents and prevented the rejection of allogeneic pancreatic islets. This approach may serve as an alternative to gene transfer-based expression with broad research and therapeutic applications
Document Actions
Personal tools