Bioluminescence Imaging
III. IVIS Imaging System
The IVIS® Spectrum Imaging System is a high-sensitivity, low noise, in vivo imaging technology platform that enables noninvasive visualization and tracking of cellular and genetic activity within a living organism in real time. The system provides both bioluminescence and fluorescence imaging capability. For fluorescence imaging, the instrument can operate in reflectance or transillumination mode. Filtered light from a broad-band lamp provides the excitation source in both modes.
In the reflectance mode, light is delivered to four reflectors that are located on the ceiling of the imaging chamber. In the transillumination mode, the excitation light is delivered to an x-y translation assembly under the stage and focused to a 2 mm diameter beam that can be directed to a particular location on the underside of the animal subject. The system also includes a structured light projector that enables reconstruction of the surface topography. The 3D location and concentration of fluorescent sources can be computed from structured light and transillumination fluorescent images.
In Vivo Molecular Imaging Core Facility
