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David Lominadze, Ph.D.

by Denise Hughes — last modified Oct 15, 2009 08:29 AM

David Lominadze

 



Curriculum Vita





DAVID LOMINADZE, Ph.D.
dglomi01@louisville.edu
Assistant Professor
Institute of Physiology, Tbilisi, Georgia, 1990

Blood cell interactions and their adhesion to vascular endothelium during cardiovascular and cerebrovascular disease.

Changes in blood rheological properties result in severe complications during many cardiovascular diseases and cerebrovascular disorders. These pathologies are inevitably accompanied with an increased content of plasma adhesion proteins (e.g. fibrinogen), enhanced platelet and leukocyte adhesion to the vascular wall and increased erythrocyte aggregability and rigidity. These changes in blood components lead to alterations in blood rheological properties and cause further complications of the diseases. Our goal is to study the mechanisms involved in blood cell interactions, platelet thrombogenesis, red blood cell aggregation, neutrophil activation and transendothelial migration, blood stasis, blood cell interactions with vascular endothelium, endothelial activation and expression of adhesion molecules and integrins, vasoreactivity, and regulated production of endothelin. Our recent studies show that increased blood content of fibrinogen increases arterial constriction by inducing increased formation of endothelin-1. Elevated blood content of fibrinogen leads to an increased macromolecular leakage in microvessels though enhanced formation filamentous actin in endothelial cells.

The experimental approaches used in my research include intravital microscopy (rats, mice), isolated and perfused microvessel methods, and cell culture. We combine these with various imaging techniques, including confocal microscopy and atomic force microscopy. In hemorheolgy studies, we use cone and plate type viscometer and flow chambers. Blood cell interactions are studied using the flow cytometry technique and confocal microscopy. In addition, biochemical and molecular analyses techniques including functional proteomics (two dimensional gel electrophoresis) are used to determine the role of plasma adhesion proteins, cellular adhesion molecules and integrins in vascular inflammatory responses during various pathological conditions such as hypertension, copper-deficiency, and ischemia/reperfusion, as well as to identify the signaling proteins in during these pathologies.

Selected publications:

  1. Lominadze D, Dean WL, Tyagi SC, Roberts AM. Mechanisms of fibrinogen-induced microvascular dysfunction during cardiovascular disease. Acta Physiologica. 2009; (In press).
    http://www.ncbi.nlm.nih.gov.echo.louisville.edu/pubmed/19723026?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
  2. Patibandla PK, Tyagi N, Dean WL, Tyagi SC, Roberts AM, Lominadze D. Fibrinogen induces alteration of endothelial cell tight junction proteins. Journal of Cellular Physiology. 2009; 221(1): 195-203.
    http://www.ncbi.nlm.nih.gov.echo.louisville.edu/pubmed/19507189?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
  3. Sen U, Tyagi N, Patibandla PK, Dean WL, Tyagi SC, Roberts AM, Lominadze D. Fibrinogen-induced production of endothelin-1 from endothelial cells. American Journal of Physiology - Cell Physiology. 2009; 296(4): C840-C847.
    http://www.ncbi.nlm.nih.gov.echo.louisville.edu/pubmed/19193866?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
  4. Tyagi N, Roberts AM, Dean WL, Tyagi SC, Lominadze D. Fibrinogen induces endothelial cell permeability.  Molecular and Cellular Biochemistry. 2008; 307(1-2):13-22.
    http://www.ncbi.nlm.nih.gov.echo.louisville.edu/pubmed/17849175?ordinalpos=13&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
  5. Tyagi N, Moshal KS, Tyagi SC, Lominadze D. GABAA receptor mitigates homocysteine-induced endothelial cell permeability. Endothelium. 2007; 14(6): 315-323.
    http://www.ncbi.nlm.nih.gov.echo.louisville.edu/pubmed/18080868?ordinalpos=10&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
  6. Lominadze D, Roberts AM, Tyagi N, Tyagi SC. Homocysteine causes cerebrovascular leakage in mice. American Journal of Physiology, Heart and Circulatory Physiology. 2006; 290: H1206-H1213.
    http://ajpheart.physiology.org/cgi/content/full/290/3/H1206
  7. Lominadze D, Tsakadze N, Sen U, Falcone JC, D’Souza SE. Fibrinogen- and its fragment D-induced vascular constriction. American Journal of Physiology, Heart and Circulatory Physiology. 2005; 288(3): H1257--H1264.
    http://ajpheart.physiology.org/cgi/content/abstract/00856.2004v1
  8. Lominadze D, Saari JT, Persival SS, Schuschke DA. Proinflammatory effects of copper deficiency on neutrophils and lung endothelial cells. Immunology and Cell Biology. 2004; 82: 231-238.
    http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=0818-9641&date=2004&volume=82&issue=3&spage=231
  9. Roberts AM. Ovechkin AV, Mowbray JG, Robinson TW, Lominadze D. Effects of pulmonary ischemia-reperfusion on platelet adhesion in subpleural arterioles in rabbits.  Microvascular Research. 2004; 67(1): 29-37.
    http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WN8-49W6PWY-2&_coverDate=01%2F31%2F2004&_alid=219173383&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6956&_sort=d&view=c&_acct=C000011238&_version=1&_urlVersion=0&_userid=134779&md5=47e37a3d6d080b50a8f14e13cd81e26d
  10. Lominadze D, Dean WL. Involvement of fibrinogen specific binding in erythrocyte aggregation. FEBS Letters. 2002; 517(1-3): 41-44.
    http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T36-45DFF3V-3-7&_cdi=4938&_orig=browse&_coverDate=04/24/2002&_sk=994829998&view=c&wchp=dGLbVzb-lSzBk&_acct=C000011238&_version=1&_userid=134779&md5=fdf7574264f82dee4ed55ea29c9b5794&ie=f.pdf
  11. Lominadze D, Joshua IG, Schuschke DA. Increased erythrocyte aggregation in spontaneously hypertensive rats. American Journal of Hypertension. 1998; 11(7): 784-789.
    http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9683038&dopt=Abstract
  12. Lominadze D, Joshua IG, Schuschke DA. In vivo platelet thrombus formation in microvessels of spontaneously hypertensive rats. American Journal of Hypertension. 1997; 10(10): 1140-1146.
    http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9370385&dopt=Abstract
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