Dr. Khundmiri's Research
Techniques and Research Projects:
Techniques
Molecular Biology: RNA isolation, genomic and restriction fragment DNA isolation, qualitative and quantitative analysis of DNA, deletion mutations, PCR amplification and RT-PCR.
Biochemical Techniques: Assay of various enzymes, protein purification, radioisotopic transports of Pi, L-Proline, and D-Glucose in BBMVs, preparation and purification of polyclonal antibodies, immunoprecipitation, electrophoresis, immunoblot techniques, plasma membrane preparations,SDS-PAGE, densitometry, Confocal microscopy, enzyme kinetics, spectrophotometry
Tissue Culture: Preparative techniques in tissue culture, maintenance of eukaryotic and insect cell lines, transient and stable transfection procedures using Geneporter.
Virology: Isolation of viral DNA and RNA from serum, nested RT-PCR, and PCR.
Recent Projects
- Regulation of Na-K ATPase by NHERF
AHA National Scientist Development Grant 07/2004-06/2008
PI: Syed Jalal Khundmiri - Regulation of Na-K ATPase by PTH in opossum kidney cells
AHA Ohio Valley Affiliate 01/2001-6/30/2003
PI: Syed Jalal Khundmiri - Analysis of PTH and Dopamine Receptor Signaling in Proximal Tubules
Department of Veterans Affairs Merit Review
10/2001 – 10/2005
PI: Eleanor D. Lederer, M.D. Post Doc: S. J. Khundmiri, Ph.D. - Regulation of Phosphate by Kidney Cells
Eagles D.D. Dunlap Kidney Fund
10/2002 – 10/2003
PI: Eleanor D. Lederer, M.D Post Doc: S. J. Khundmiri, Ph.D. - Regulation of sodium-phosphate co-transport in proximal renal tubule cells
Department of Veterans Affairs Merit Review
4/1997 – 03/2000
PI: Eleanor D. Lederer, M.D. - Post Doc: S. J. Khundmiri, Ph.D. - Analysis of PTH and Dopamine Receptor Signaling in Proximal Tubules
National Kidney Foundation Research Fellowship
PI: Syed Jalal Khundmiri, Ph.D. - Sponsor: Eleanor D. Lederer, M.D.
7/01/2000 – 06/30/2002 - Role of a Kinase Anchoring Protein in the Regulation of Sodium-Dependent Phosphate Transport
Intramural Research Incentive Grants Program
PI: Eleanor D. Lederer, M.D. - Post Doc: S. J. Khundmiri, Ph.D.
04/01/2000 – 03/31/2001
