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Dr. Bhatnagar's CV

by c0li0003 — last modified Aug 01, 2010 12:35 PM

CURRICULUM VITAE

Updated to APRIL 2001

Aruni Bhatnagar, Ph.D.

Professor

Division of Cardiology

Department of Medicine

University of Louisville

Room 107, Jewish Cardiovascular Research Center
500 South Floyd
Louisville, KY 40202
Tel: (502) 852-4883, (502) 852-5596
Fax: (502) 852-1795
Email:aruni@louisville.edu

 

 

UofL Cardiology - A. Bhatnagar

BIOGRAPHICAL:

DATE & PLACE OF BIRTH: Dec. 17, 1960 - Lucknow, U.P. India

CITIZENSHIP:   Indian (Permanent Resident USA)

HOME ADDRESS:      3405, Mt. Rainier Drive
                                     Louisville, KY 40241
                                     (502) 339-7450

EDUCATION:

        Institution                                 Date                   Degree                  Subject
University of Lucknow,  India        1979                      B.S.                Chemistry, Botany, Zoology 
University of Lucknow,  India        1981                      M.S.               Biochemistry
University of Kanpur, India            1986                      Ph.D.              Chemistry

 

UofL Cardiology - A. Bhatnagar

PROFESSIONAL EXPERIENCE:

1981 - 1985 Research Fellow National Council of Education Research & Training,  Central Drug Research Institute Lucknow, U.P. 226001 India
1985 - 1990 Postdoctoral Fellow Dept. of Human Biological Chemistry & Genetics University of Texas Medical Branch, Galveston, TX 77555
1990 - 1995 Assistant Professor Dept. of Human Biological Chemistry & Genetics and Dept. of      Physiology and Biophysics, University of Texas Medical Branch,  Galveston, TX 77555
1995 - 1998 Associate Professor Dept. of Human Biological Chemistry & Genetics and Dept. of    Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX 77555
1998 - to date Professor  Division of Cardiology, Department of Medicine, and the Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY40202

Teaching:

Member Graduate School, Department of Human Biological Chemistry and Genetics, UTMB, 1990-1998.
Special Member Graduate School, Department of Physiology and Biophysics, UTMB, 1991-1998.
School of Medicine (UTMB); 1990-1998:
Clinical Correlation; 1 lecture
Small Group Exercises (IFL) in Human Genetics: (1 week course)
Clinical Exercises (2 to 3 per year)
Graduate School (UTMB): 1992-1998
Citric Acid Cycle and Oxidative Phosphorylation-Cell Biology Course (4 lectures).
Carbohydrate Metabolism: Department of Human Biological Chemistry and Genetics (6 lectures)

Students/Trainees

1. Si-Qi Liu, Graduate Student, HBC&G (Co-Supervisor) UTMB, 1991 - 1995
2. Albert Zacarias, Ph.D., Post-Doctoral Fellow, Physiology and Biophysics, UTMB,1992 - 1998.
3. Animesh Chandra, Ph.D., Post-Doctoral Fellow, HBC&G, UTMB, 1994-1997
3. Li-Fei Wang, M.D., Post-Doctoral Fellow, HBC&G, UTMB, 1994 - 2000.
3. Kshama Kumari, Ph.D., Post-Doctoral Fellow, HBC&G, UTMB, 1992 - 1994.
4. Jeff Almrud, Student, Texas A&M, 1992 - 1994.
5.Sanjay Srivastava, Ph.D., Post-Doctoral Fellow, HBC & G, UTMB, 1995-1998
6. Xiaoging Yang, Graduate Student, (graduate rotation), Physiology and Biophysics, UTMB 1991.
7. Johannes Ruef, M.D., Fellow, Division of Cardiology, UTMB, 1996-1998.
8. Bharat Dixit, Ph.D., Post-Doctoral Fellow, UTMB, 1998-2000.
9. Kota Ramana, Ph.D., Post-Doctoral Fellow , HBC&G, UTMB, 1999-present.
10. Hong-jun Jing, M.S., Fellow, Division of Cardiology, University of Lousiville, 1999-2001.
11. Ken Shinmura, M.D., Fellow, Division of Cardiology, University of Louisville, 1999-2000
 

SERVICE

Interview students and faculty for School of Medicine.
Scientific Review: Biochemical Pharmacology
    Comparative Biochemistry and Physiology
    Toxicology and Applied Pharmacology
    Journal of Molecular and Cellular Cardiology
Experimental and Clinical Cardiology
Journal of Pharmacology and Experimental Therapeutics
Circulation Research
Biochemistry
Editorial Board. Journal of Pharmacology and Experimental Therapeutics. Aug, 1999 -
Grant Review:  Sealy Center Grants at the University of Texas
Jewish Heart and Lung Institute, Louisville
Charter Member: DRG1 ALTX-4 Study Section, National Institutes of Health, 1999-2002
  

MEMBERSHIP IN SCIENTIFIC SOCIETIES:

International Society of Heart Research
International Society of Eye Research (ISER)
American Heart Association
AWARDS AND HONORS:
National Science Talent Award, India, 1976.
Summer Fellowship, National Council of Educational Research and Training, India 1977, 1979, 1981
University Scholar, University of Louisville, Louisville, KY 1998
PH.D THESIS TITLE:
Biochemical and Electrophysiological Characterization of the Pacemaker Cells of the Sino-Atrial Node and its Relation to the Origin of Heart Beat.

RESEARCH ACTIVITIES

A. AREAS OF INTEREST:
Oxidative Mechanism of Cardiovascular Disease
Secondary Complications of Diabetes
Redox Regulation of Gene Expression
Cellular Cardiac Electrophysiology
 Role of Lipid Peroxidation in Atherosclerosis
 Oxidative DNA Damage
 
B. RESEARCH SUPPORT:
As Principal Investigator:
 
 1. Mechanism of Free Radical Induced Myocardial Injury
  National Institutes of Health R29 HL44676:  4/1/90 - 3/30/95:   Annual Direct Costs   $ 75,000,  Total Direct Costs   $ 350,000,  Status: Completed
 
2. Attenuation of Free Radical Injury to Myocardial Cells by Low Frequency Electromagnetic  Fields
  Electro-Biol Inc.:  10/1/92 - 12/1/94   Total Direct Costs  $80,000, Status: Completed

3. A Cell Culture Model of Age-Related Oxidative Changes in Mitochondrial Genome and  Cellular Electrophysiology
 UTMB Center on Aging,   5/1/93 - 4/20/94   Total Direct Costs  $10,000,  Status: Completed

 4. Use of Externally Applied Magnetic Fields to Limit Free Radical Injury during Myocardial  Reperfusion
 Texas Advance Technology Program (# 004952057),  1/1/94 - 12/31/96   Annual Direct Costs  $ 56,770. Total Direct Costs  $117,540.  Status: Completed

 5. Detoxification and Toxicity of 4-Hydroxyalkenals in Heart (35 % effort)
National Institutes of Health, RO1 HL55477-04, 4/1/97-3/31/01,  Current Annual Direct Costs $ 180,913. Total Direct Costs  $ 636, 534. Status: completed
National Institutes of Health, RO1 HL55477-05, 4/1/01-3/31/06,  Current Annual Direct Costs $ 225,000. Total Direct Costs  $ 1,125,000. Status: Funded
 
6. Cardiac Metabolism of Enals (35 % effort)
National Institutes of Health RO1 HL/ES59378-01, 12/01/97-11/30/02, Current Annual Direct Costs $145,873.  Total Direct Costs  $ 774,466. Status: Funded

7. Cardioprotective Effects of Ethanol (10 %)
 National Institutes of Health R21 AA13299-01,  04/01/01 - 03/31/04 Annual Direct Costs  $ 100,000.  Total Direct Costs   $ 300,000.  Status: Pending

As Co-investigator
1. Aldose Reductase and Diabetic Complications" (P.I., S.K. Srivastava)
National Institutes of Health RO1 DK36118-08, 7/1/98 - 6/31/02   Current Annual Costs  $ 210,420. Total Direct Costs  $ 1,208,506. Status: Funded

As Collaborator
1. Characterization of Genomic Changes in the Cardiac Mitochondria of Aged mice (P.I. Sanjay Srivastava)
 Grant-in-Aid, School of Medicine, University of Louisville,  4/1/2000-3/31/2001    Total Direct Costs  $ 15,000. Status: Funded
2. PKC, and Src PTK signaling in preconditioning (P.I. PeiPei Ping) 5 % Effort
  National Institutes of Health RO1 HL63901,  12/01/1999 - 11/30/2004   Annual Direct Costs  $ 219,013.  Total Direct Costs  $ 1,193,851. Status: Funded
 
 3. $-Adrenergic Axis and Oxidative Stress in Heart Failure (P.I. Sumanth D. Prabhu) 5 % effort
  National Institutes of Health,  07/01/01 - 06/30/06    Annual Direct Costs $ 225,000. Total Direct Costs  $ 1,125,000.  Status: Pending
 
As Consultant
1. The Role of Aldose Reductase in Vascular Smooth Muscle Cell Growth (P.I. Si.- Qi Liu)
 American Heart Association, Ohio Valley Affiliate,  7/1/1999 to 6/20/2001     Annual Direct Costs  $ 30,000. Total Direct Cost  $ 60,000.  Status: Funded
2. Contribution of Lipid Peroxidation Products to the Mitogenic Effects of Oxidized Low Density Lipoprotein (P.I. Sanjay Srivastava).
 American Heart Association-Ohio Valley Affiliate Annual Direct Costs  $ 35,000.  07/01/2000-06/30/2002    Total Direct Costs  $ 70,000. Status: Funded
 3. Mitochondrial Metabolism of Aldehydes (P.I. Si-Qi Liu)
 American Heart Association- National-Scientist Development Grant,  07/01/00-06/30/04     Annual Direct Costs  $ 59,091.  Total Direct Costs  $ 236,364
 Status: Funded
 3. The Role of Aldose Reductase in Vascular Smooth Muscle Cell Growth (P.I. Si.-Qi Liu)
 National Institutes of Health RO1 HL64849-01. 12/01/2000-11/31/2005    Annual Costs   $ 200,000. Total Direct Cost  $ 1,000,000. Status: Pending
 

PUBLICATIONS:

  1. Bhatnagar, A., Mehrotra, M. and Tripathi, O. Calcium efflux and spontaneous activity of frog sinus venosus in isotonic sucrose solution. Ind. J. Exp. Biol. 24, 389-394, 1986.
  2. Awasthi, Y.C., Bhatnagar, A. and Singh, S.V. Evidence for the involvement of histidine at the active site of glutathione S-transferase from human liver. Biochem. Biophys. Res. Comm. 143, 965-970, 1987.
  3. Bhatnagar, A., Das, B. and Srivastava, S.K. Diethyl pyrocarbonate inactivation of human placental aldehyde reductase II. Biochim. Biophys. Acta 916, 179-184, 1987.
  4. Bhatnagar, A., Das, B., Gavva, S.R., Cook P.F. and Srivastava, S.K. The kinetic mechanism of human placental aldose reductase and aldehyde reductase II. Arch. Biochem. Biophys. 261, 264-274, 1988.
  5. Tripathi, O. and Bhatnagar, A. Extracellular fluid space in rabbit SA node and atria as studied by 14C-inulin and 14C-sucrose. Jpn. J. Physiol. 37, 1057-1060, 1987.
  6. Tripathi, O., Ray, M., Bajpai, V.K., Bhatnagar, A., Kumar, R., Singh, Y.K., Singh, C., Shipstone, A.C. and Dhawan, B.N. Effects of sodium deoxycholate on the mechanical and electrical activities and ultrastructure of guinea pig atria. Biomed. Biochim. Acta 9, 901-914, 1988.
  7. Das, B., Bhatnagar, A., Liu, S-Q., Srivastava, S.K., Messmer, M., Ueno, N. and Chakrabarti, B. Spectral properties of human placental aldose reductase and aldehyde reductase II. Biochem. Int. 19, 497-504, 1989.
  8. Srivastava, S.K., Ansari, N.H., Liu, S-Q., Izban, A., Das, B., Szabo, G. and Bhatnagar, A. The effect of oxidants on biomembranes and cellular metabolism. Mol. Cell. Biochem. 91, 149-157, 1989.
  9. Srivastava, S.K., Ansari, N.H., Bhatnagar, A., Hair, G., Liu, S-Q. and Das, B. Activation of aldose reductase by nonenzymatic glycosylation. In Maillard Reaction in Aging, Diabetes, and Nutrition. Publisher Alan R. Liss, Inc. pp. 171-184, 1989.
  10. Liu, S-Q., Bhatnagar, A., Das, B. and Srivastava, S.K. Functional cysteinyl residues in human placental aldose reductase. Arch. Biochem. Biophys. 275, 112-121, 1989.
  11. Bhatnagar, A., Liu, S-Q., Das, B. and Srivastava, S.K. Involvement of sulfhydryl residues in aldose reductase-inhibitor interaction. Mol. Pharmacol. 36, 825-830, 1989.
  12. Bhatnagar, A., Liu, S-Q., Das, B., Ansari, N.H. and Srivastava, S.K. Inhibition kinetics of human kidney aldose and aldehyde reductases by aldose reductase inhibitors. Biochem. Pharmacol. 39, 1115-1124, 1990.
  13. Bhatnagar, A., Srivastava, S.K. and Szabo, G. Oxidative stress alters specific membrane currents in isolated cardiac myocytes. Circ. Res. 67, 535-549, 1990.
  14. Srivastava, S.K., Ansari, N.H. and Bhatnagar, A. Sugar induced cataractogenesis: A paradigm of oxidative tissue pathology? Lens Eye Toxic. Res. 7, 161-171, 1990.
  15. Bhatnagar, A., Liu, S-Q. and Srivastava, S.K. Structure-activity correlations in human kidney aldehyde reductase-catalyzed reduction of para-substituted benzaldehyde by 3-acetyl pyridine adenine dinucleotide phosphate. Biochim. Biophys. Acta 1077, 180-186, 1991.
  16. Bhatnagar, A., Das, B., Liu, S-Q. and Srivastava, S.K. Human liver aldehyde reductase: pH dependence of steady-state kinetic parameters. Arch. Biochem. Biophys. 287, 329-336, 1991.
  17. Ansari, N.H., Bhatnagar, A., Liu, S-Q. and Srivastava, S.K. Purification and characterization of aldose reductase and aldehyde reductase from human kidney. Biochem. Int. 25, 755-765,   1991.
  18. Liu, S-Q., Bhatnagar, A. and Srivastava, S.K. Carboxymethylation-induced activation of   bovine lens aldose reductase. Biochim. Biophys. Acta 1120, 329-336, 1992.
  19. Bhatnagar, A. and Srivastava, S.K. Aldose reductase: Congenial and injurious profiles of an   enigmatic enzyme. Biochem. Med. Metabol. Biol. 48, 91-121, 1992.
  20. Bhatnagar, A., Liu, S-Q., Petrash, J.M. and Srivastava, S.K. Mechanism of inhibition of aldose reductase by menadione (Vitamin K3). Mol. Pharmacol. 42, 917-921, 1992.
  21. Liu, S-Q., Bhatnagar, A. and Srivastava, S.K. Does sorbinil bind to the substrate binding site  of aldose reductase? Biochem. Pharmacol. 44, 2427-2429, 1992.
  22. Petrash, J.M., Harter, T.M., Devine, C.S., Olins, P.O., Bhatnagar, A., Liu, S-Q. and Srivastava, S.K. Involvement of cysteine residues in catalysis and inhibition of human aldose reductase:   Site-directed mutagenesis of Cys-80, -298 and -303. J. Biol. Chem. 267, 24833-24840, 1992.
  23. Castro, G.J., and Bhatnagar, A. Effect of extracellular ions and modulators of calcium transport on survival of tert-butyl hydroperoxide exposed cardiac myocytes. Cardiovas. Res. 27, 1873-1881, 1993
  24. Liu, S-Q., Bhatnagar, A., Ansari, N.H., and Srivastava, S.K. Identification of the reactive cysteine residue in human placenta aldose reductase. Biochim. Biophys. Acta. 1164, 268-272,   1993
  25. Bhatnagar, A., Ansari, N.H., Zacarias, A. and Srivastava, S.K. Digital image analysis of cultured rat lens during oxidative stress-induced cataractogenesis. Exp. Eye Res. 57, 385-391,1993.
  26.  Liu, S-Q., Bhatnagar, A. and Srivastava, S.K. Bovine lens aldose reductase: pH-Dependence of steady-state kinetic parameters and nucleotide binding. J. Biol. Chem. 268, 25494-25499,   1993.
  27. Bhatnagar, A., Liu, S-Q., Ueno, N., Chakrabarti, B. and Srivastava, S.K. Human placental   aldose reductase: Role of Cys-298 in substrate and inhibitor binding. Biochim. Biophys. Acta   1205, 207-214, 1994.
  28. Ansari, N.H., Bhatnagar, A., Fulep, E., Khanna, P. and Srivastava, S.K. Trolox protects hyperglycemia-induced cataractogenesis in cultured rat lens. Res. Comm. Chem. Pathol. Pharmacol. 84, 93-104, 1994.
  29. Bhatnagar, A. Biochemical mechanism of irreversible cell injury caused by free radical-initiated reactions. Mol. Cell Biochem. 137, 9-16, 1994.
  30. Bhatnagar, A. Electrophysiological effects of 4-hydroxynonenal, an aldehydic product of lipid peroxidation, on isolated rat ventricular myocytes. Circ. Res. 76, 293-304, 1995
  31. Bhatnagar, A. Attenuation of reperfusion injury by the antioxidant n-propyl gallate. J.   Cardiovasc. Pharmacol. 26, 343-347, 1995.
  32. Bhatnagar A. Ansari N.H, Wang L-F, Khanna P, Wang C-S, Srivastava S.K. Calcium-mediated disintegrative globulization mimics cataractogenesis. Exp Eye Res. 61, 303-310 1995.
  33. Bhatnagar A. Metabolic dependence of free radical-induced hypercontracture of cardiac myocytes. Biochem. Arch. 11, 135-140, 1995.
  34. Srivastava, S., Chandra A., Bhatnagar, A., Srivastava, S.K., Ansari, N.H. Lipid peroxidation product, 4-hydroxynonenal and its conjugate with GSH are excellent substrates of bovine lens aldose reductase. Biochem. Biophys. Res. Comm. 217, 741-746, 1995.
  35. Wang, L.F., Bhatnagar, A., Ansari, N.H., Dhir, P., Srivastava, S.K. Mechanism of calcium-induced disintegrative globulization of rat lens fiber cells. Invest. Ophthalmol. Vis. Sci. 37, 915-922, 1996.
  36. Srivastava, S.K., Awasthi, S., Wang L.F., Bhatnagar, A., Awasthi, Y.C., Ansari, N.H. Attenuation of 4-hydroxynonenal-induced catractogenesis in rat lens by butylated hydroxytoluene. Curr. Eye Res. 15, 749-754, 1996.
  37. Srivastava, S.K., Wang L.-F., Ansari, N.H. and Bhatnagar, A. Calcium homoestasis in isolated single cortical fibers of rat lens. Invest. Ophthalmol. Vis. Sci. 38, 2300-2312, 1997.
  38. Bhatnagar, A., Dhir, P., Wang, L.-F., Lo, W.-K., and Srivastava, S. K. Alterations in the light transmission through single lens fibers during calcium-mediated disintegrative globulization. Invest. Ophthalmol. Vis. Sci. 38, 586-592, 1997.
  39. Bhatnagar A. Contribution of ATP to oxidative stress-induced changes in the action potential of isolated cardiac myocytes. Am. J. Physiol. 272, H1598-H1608, 1997.
  40. Chandra, A., Srivastava, S., Petrash, M., Bhatnagar, A., and Srivastava, S.K. Active site modification of aldose reductase by nitric oxide. Biochim. Biophys. Acta 1341, 217-222, 1997.
  41. Wang, L.F, Dhir, P., Bhatnagar, A., Srivastava, S.K. Contribution of osmotic changes to disintegrative globulization of single cortical fibers isolated from rat lens. Exp. Eye Res. 65, 267-275, 1997.
  42. Chandra, A., Srivastava, S., Petrash, M., Bhatnagar, A., Srivastava, S.K. Modification of aldose
  43. reductase by nitrosoglutathione. Biochemistry 36, 15801-15809, 1997
  44. Bhatnagar, A., Hypercontracture of cardiac myocytes induced by the lipid peroxidation product, 4-hydroxynonenal: dependence on external calcium and energy source. Biochem. Arch.13, 215-222 1997.
  45. Reuf J., Rao, G.N., Li F.Z., Bode, C., Patterson, C., Bhatnagar, A., and Runge, M.S. Induction of aortic smooth muscle cell growth by the lipid peroxidation product, 4-hydroxy-2-nonenal. Circulation 97, 1071-1078, 1998.
  46. Srivastava S, Chandra A, Ansari N.H, Srivastava S.K, Bhatnagar, A. Identification of cardiac oxidoreductase(s) involved in the metabolism of the lipid peroxidation derived aldehyde - 4-hydoxynonenal. Biochem. J. 329, 469-475, 1998.
  47. Srivastava S. Chandra A, Wang, L.-F., Seifert, W.E., DaGue, B.B., Ansari, N.H., Srivastava, S.K.,Bhatnagar, A. Metabolism of the lipid peroxidation product, 4-hydoxy-trans-2-nonenal, in isolated perfused rat heart. J. Biol. Chem. 273, 10893-10900, 1998.
  48. Srivastava S., Harter, T.M., Chandra, A., Bhatnagar, A., Srivastava, S.K., and Petrash, J. M. Kinetic studies of FR-1, a growth factor-inducible aldo-keto reductase. Biochemistry 37, 12909-12917, 1998.
  49. Bhatnagar, A., Srivastava, S., Wang, L., Chandra, A., Ansari, N.H., Srivastava, S.K. Cardiac metabolism of enals. Adv. Exp. Med. Biol. 463, 223-229, 1999..
  50. Petrash, J.M., Harter, T.M., Srivastava, S., Chandra, A., Bhatnagar, A., Srivastava, S.K. Strucuture-Function studies of FR-1: A growth factor-inducible aldo-keto reductase. Adv. Exp. Med. Biol. 463, 435-443, 1999.
  51. Srivastava, S.K., Chandra, A., Srivastava, S., Petrash, J.M., Bhatnagar, A., Regulation of aldose reductase by aldehydes and nitric oxide. Adv. Exp. Med. Biol. 463, 501-507, 1999.
  52. Srivastava, S., Watowich, S.J., Petrash, J.M., Srivastava, S.K. and Bhatnagar, A. Structural and kinetic determinants of aldehyde reduction catalyzed by aldose reductase. Biochemistry 38, 42-54, 1999.
  53. Bhatnagar, A., and Bolli, R. Modulation of KATP channels to protect the ischemic myocardium: Clinical implications. Exp. Clin. Cardiol. 4, 20-22, 1999.
  54. Ruef, J., Liu, S.-Q., Bode, C., Tocchi, M., Srivastava, S., Runge, M., and Bhatnagar, A. Involvement of aldose reductase in vascular smooth muscle cell growth and lesion formation after arterial injury. Arterio. Thromb. Vasc. Biol. 20, 1745-1752, 2000.
  55. Dixit, B.L., Balendiran, G.K., Watowich, S.J., Srivastava, S., Ramana, K.V., Petrash, J.M. Bhatnagar, A., and Srivastava, S.K. Kinetic and structural characterization of the glutathione binding site of aldose reductase. J. Biol. Chem. 275, 21587-21595, 2000
  56. Wang, L.-F., Christensen, B.N., Bhatnagar, A., and Srivastava, S.K. Calcium-dependent protease(s) mediate globulization of isolated rat cortical fiber cells. Invest. Ophthalmol Vis Sci. 42, 194-199, 2001.
  57. Shinmura, K., Tang, X-L, Wang, Y., Xuan, Y-T, Liu, S-Q, Takano, H., Bhatnagar, A., and Bolli, R. Cyclooxygenase-2 mediates the cardioprotective effects of the late phase of ischemic preconditioning in conscious rabbits. Proc. Natl. Acad Sci. USA 97, 10197-10202, 2000.
  58. Ramana K.V., Dixit, B.L., Srivastava, S.K., Balendiran G.K, Watowitch S.J., Petrash J.M., Bhatnagar, A., and Srivastava, S.K. Characterization of the glutathione binding site of aldose reductase. Adv. Exp. Med. Biol. (In Press) 2000
  59. Dixit, B., Kota R., Jackson, EB., Srivastava, S., Bhatnagar, A., and Srivastava, S.K., Metabolic regulation of AR activity by nitric oxide donors. Adv. Exp. Med. Biol. (In Press) 2000.
  60. Ramana K.V., Dixit, B.L., Srivastava, S, Balendiran G.K., Srivastava, S.K. and Bhatnagar, A. Selective recognition of glutathiolated aldehydes by aldose reductase. Biochemistry 39, 12172-12180, 2000.
  61. Bhatnagar, A., Ruef Johannes, Liu, S.-Q., Srivastava, S, and Srivastava, S.K. Regulation of vascular smooth muscle cell growth by aldose reductase. Adv. Exp Med. Biol. (In Press) 2000
  62. Srivastava, S, Liu, S.-Q., Zacarias A., Srivastava, S, and Bhatnagar. A. Involvement of aldose reductase in the endothelial metabolism of atherogenic aldehydes. Adv. Exp. Med. Biol. (In Press) 2000
  63. Liu, S.-Q., Jin, H., Zacarias, A., Srivastava, S., and Bhatnagar, A. Binding of pyridine coenzymes to the beta-subunit of the voltage-sensitive potassium channels. Adv. Exp. Med. Biol. (In Press) 2000.
  64. Srivastava, S.K., Dixit, B.L., Srivastava, S., Ramana, K.V., Balendiran, G., and Bhatnagar, A., Aldose reductase efficiently detoxifies lipid-derived aldehydes. Pharmacol Therap New Millen. (In Press)
  65. Srivastava, S., Dixit, B.L., Cai, J., Sharma, S., Hurst, H., Bhatnagar, A., and Srivastava, S.K., Metabolism of lipid peroxidation product, 4-hydroxynonenal (HNE) in rat erythrocytes - Role of aldose reductase. Free Radic. Biol. Med. 29, 642-651, 2000.
  66. Srivastava, S., Dixit, B., Ramana, K.V., Chandra, A., Petrash, J.M., Bhatnagar, A., and Srivastava, S. K.. Kinetic and structural modifications of aldose reductase by nitrosothiols. Biochem. J. (In Press) 2001.
  67. Srivastava, S., Conklin, D.J., Boor, PJ, Liu, S.Q., Srivastava, S.K., and Bhatnagar, A. Identification of biochemical pathways for the metabolism of oxidized low-density lipoprotein-derived aldehyde - 4-hydroxy trans-2-nonenal in vascular smooth muscle cells. Atherosclerosis (In Press) 2001.
  68. Liu, S.Q., Jin, H., Zacharias, A., Srivastava, S., Bhatnagar, A. Binding of pyridine coenzymes to the $-subunit of the voltage-sensitive potassium channel. J. Biol. Chem. (In Press) 2001.

 

 

ABSTRACTS:

  1. Bhatnagar, A. Bioelectrochemical studies on the cardiac muscle. Proc. IIIrd Congress of Indian Association of College-going-Scientists., Ind. Inst. Tech., Kanpur, India. 26-27, 1980.
  2. Bhatnagar, A. and Tripathi, O.N. The contracture of frog ventricular strips in non-electrolytic sucrose solution. Ind. J. Heart Res., 1, 45, 1980.
  3. Bhatnagar, A., Chowdury, A.R. and Tripathi, O.N. Relationship between the ion content and mechanical activity of frog ventricular strips soaked in ion-free sucrose solution. Ind. J.   Pharmacol., 14, 50, 1982.
  4. Bhatnagar, A., Mehrotra, M., Singh, C., and Tripathi, O.N. Altered response pattern of isolated auricles to vagosymapathetic nerve stimulation in the presence of deoxycholic acid. Ind. J.   Pharmacol., 14, 54 , 1982
  5. Bhatnagar, A., Kapoor, N.K., Singh, C., and Tripathi, O.N. Differential activity of calcium-ATPase in amphibian and mammalian myocardium and its modification by sucrose solution and deoxycholate. Ind. J. Pharmacol. 14, 49, 1982.
  6. Mehrotra, M., Bhatnagar, A., Tripathi, O.N., and Dhawan, B.N. Effects of lanthanum on the   response of isolated guinea-pig auricles to vagosympathetic stimulation. Ind. J. Pharmacol., 15, 25, 1983.
  7. Bhatnagar, A., and Tripathi, O. Effects of nifedipine on the spontaneous electrical activity of   rabbit SA node. Ind. J. Heart Res., 2, 27, 1984.
  8. Bhatnagar, A., and Tripathi, O. Effects of A-23187 on the transmembrane electrical activity of the pacemaker cells of rabbit SA node. J. Mol. Cell. Cardiol., 18, 12, 1986.
  9. Bhatnagar, A., and Tripathi, O. A change in the action potential duration does not underlie the negative chronotropic effect of acetylcholine on rabbit SA node. J. Mol. Cell. Cardiol., 18, 71, 1986.
  10. Das, B., Ansari, N.H., Hair, G., Bhatnagar, A. and Srivastava, S.K., Role of aldose reductase in diabetic complications. Fed. Proc. 45, 1502, 1986.
  11. Bhatnagar, A., Das, B., Cook, P.F and Srivastava, S.K. Steady-state kinetics of aldose reductase reaction. Invest. Opthalmol Vis Sci, 28, 281, 1987.
  12. Song, H.P., Das, B., Bhatnagar, A., and Srivastava, S.K. Purification and properties of human brain, lens and placenta. Fed. Proc., 46, 1495, 1987.
  13. Bhatnagar, A., Das, B., and Srivastava, S.K. Structure-activity studies on aldose reductase and aldehyde reductase II, Fed. Proc., 46, 1971, 1987.
  14. Das, B., Bhatnagar, A., and Srivastava, S.K. Diethylpyrocarbonate inactivation of human   placental aldehyde reductase II, Fed. Proc., 46, 2154, 1987.
  15. Srivastava, S.K., Das, B., Liu, S-Q., Ansari, N.H. and Bhatnagar, A. Inhibition of human kidney aldose reductase and aldehyde reductase by aldose reductase inhibitors. Invest. Ophthalmol Vis Sci, 30, 1989.
  16. Liu, S-Q., Bhatnagar, A., Das, B., and Srivastava, S.K. pH dependence of kinetic parameters of human liver aldehyde reductase. FASEB J, 4, A1164, 1990.
  17. Srivastava, S.K., Ansari, N.H., Das, B., Liu, S-Q., and Bhatnagar, A. Induction of aldose   reductase by glucose in bovine lens and the mechanism of enzyme reaction. Invest Opthalmol Vis Sci , 31, 1990.
  18. Srivastava, S.K., Liu, S-Q., Ansari, N.H. and Bhatnagar, A. Oxidative stress modulates inhibitor sensitivity of bovine lens aldose reductase. Invest Opthalmol Vis Sci, 32, 1011, 1991.
  19. Bhatnagar A., Liu, S-Q., and Srivastava, S.K. Approaches to increase selectivity of aldose   reductase inhibitors. Transition state analogs and substrate enzyme complexes. U.S.-Japan   Aldose Reductase Workshop; Kona, Hawaii, 1991.
  20. Srivastava, S.K., Liu, S-Q., Bhatnagar, A. and Ansari, N.H. Mechanism of activation of human and bovine lens aldose reductase. U.S.-Japan Aldose Reductase Workshop; Kona, Hawaii,   1991.
  21. Srivastava, S.K., Ansari, N.H., Liu, S-Q., and Bhatnagar, A. Regulation of aldose reductase activity and inhibitor sensitivity by sulfhydryl oxidation. FASEB J, 5, A1507, 1991.
  22. Srivastava, S.K., Liu, S-Q., Bhatnagar, A. and Ansari, N.H. Activation of bovine lens aldose   reductase by carboxymethylation. Int Cong Eye Res, 1992.
  23. Bhatnagar, A., Liu, S-Q., Ueno, N., Chakrabarti, B. and Srivastava, S.K. Role of Cys-299 in   catalysis and inhibition of human placental aldose reductase. FASEB J, 6, A1699, 1992.
  24. Srivastava, S.K., Ansari, N.H., Liu, S-Q. and Bhatnagar, A. Carboxymethylation-induced activation of bovine lens aldose reductase. Invest Opthalmol Vis Sci, 33, 1376, 1992.
  25. Srivastava, S.K., Liu, S-Q., Bhatnagar, A., Ansari, N.H. and Petrash, J.M. Sorbinil and alcohol substrate bind at the same site on bovine lens aldose reductase. Invest Opthalmol Vis Sci, 34, 758, 1993.
  26. Fulep, E., Ansari, N.H., Bhatnagar, A., Zacarias, A. and Srivastava, S.K. Digital image analysis of cultured rat lens during oxidative stress-induced cataractogenesis. Investi Opthal mol Vis Sci, 34, 1297, 1993.
  27. Bhatnagar, A. Hydrogen peroxide-induced alterations in the kinetics of the cardiac sodium current. Biophys J, 64, A91 1993
  28. Bhatnagar, A. Tertiary-butyl hydroperoxide (t-BHP)-induced alterations in the cardiac action potential, FASEB J, 7, A102, 1993
  29. Ansari, N.H., Bhatnagar, A., Zacarias, A. and Srivastava, S.K. Prevention of sugar-induced cataractogenesis in rat lenses by Trolox. FASEB J, 7, A848, 1993.
  30. Liu, S-Q., Bhatnagar, A., Petrash, J.M. and Srivastava, S.K. Role of Cys-298 in inhibition of   aldose reductase by menadione. FASEB J, 7, A848, 1993.
  31. Zacarias A, Simon B, Bhatnagar A. Magnetic Field effects on metal-catalyzed reactions: Luminol radical recombination in solution. Biophys J, 66, A377, 1994
  32. Almrud JJ, Bhatnagar A. Activation of ATP-sensitive K channel in cardiac myocytes under oxidative stress: role of the DNA repair enzyme poly (ADP-ribose) polymerase. Biophys J, 66, A329, 1994.
  33. Ansari, N.H., Bhatnagar, A. and Srivastava, S.K. Digital image analysis: A powerful tool for  the quantitation of endogenous peroxides and lens opacity. Invest Opthalmol Vis Sci, 35, 1810, 1994.
  34. Bhatnagar, A. Aldehydic product of lipid peroxidation, 4-hydroxynonenal, inhibits inward rectifies K+ channels and depletes intracellular ATP in isolated rat ventricular myocytes. FASEB J, 8, A607, 1994.
  35. Liu, S-Q., Bhatnagar, A., Ansari, N.H. and Srivastava, S.K. Catalytic reaction mechanism of aldose reductase. FASEB J, 8, A936. 1994.
  36. Srivastava, S.K., Ansari, N.H., Wang, L. and Bhatnagar, A. Isolation and culture of single rat lens fiber cells: Effect of calcium. Int Cong Eye Res, 1994.
  37. Srivastava, S. K., Ansari, N.H., Khanna, P., Wang, C., Bhatnagar, A. Calcium channel and Na+/ Ca++ exchanger and Ca++ -induced globulization of isolated lens fiber cells that mimic cataractogenesis. Invest Opthalmol Vis Sci 36, S604, 1995.
  38. Srivastava, S., Chandra, A., Ansari, N.H., Bhatnagar, A., Srivastava, S.K. Detoxification of lipid peroxidation product 4-hydroxynonenal by aldose reductase. FASEB J., 10, A272, 1996.
  39. Bhatnagar, A. High intracellular ATP delays hydrogen peroxide mediated loss of excitability of cardiac myocytes but exacerbates proongation of the action potential. FASEB J., 10 , A582, 1996.
  40. Srivastava, S.K., Awasthi S., Bhatnagar, A., Awasthi, Y.C. Lens of curcumin fed rats are resistant to the catractogenic effects of 4-hydroxynonenal in organ cultures. Invest Opthalmol Vis Sci 37, S882, 1996
  41. Reuf, J., Wu, Y., Bhatnagar, A., Bode, C., Runge, M.S., Rao, G.N. A role of 4-hydroxynonenal, a lipid peroxidation product in redox-sensitive vascular smooth muscle cell growth. Circulation Suppl 94, I637-638, 1996.
  42. Srivastava, S.K., Wang, L.-F., Bhatnagar, A. Calcium homeostasis of isolated cortical fibers of rat lesns. Invest Opthalmol Vis Sci 38, S703, 1997.
  43. Ansari, N.H., Khanna, P., Bhatnagar, A., Bhalla, P., Szweda, L. Protein-HNE adducts in human diabetic cataractous lenses. Invest Opthalmol Vis Sci 38, S1171, 1997
  44. Ruef, J., Hu, Z., Bode, C., Runge, M., Bhatnagar, A. Role of aldose reductase in growth factor-induced proliferation of vascular smooth muscle cells. J. Amer. Coll. Cardiol. (In Press), 1997.
  45. B. Dixit, S. Srivatava, S. Watowich, J.M. Petrash, A. Bhatnagar, and S.K. Srivastava. Reduction of glutathione conjugates of lipid-derived unsaturated aldehydes: A novel antioxidant role of aldose reductase FASEB J. 13, A1363, 1999.

 

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