Core Facility Director: Robert Miller
Associate Director and Application Specialist: Qingsheng Li
The Microbiology and Immunology is located in the Clinical and Translational Research (CTR) building room 651 and provides all investigators at the University of Louisville, or interested outside concerns, training and access to high quality affordable flow cytometry services.
Recent upgrades to state of the art equipment include a BD LSRFortessa configured with a total of 4 lasers: 355nm (UV), 488nm (blue), 405nm (violet) and 640nm (red) supporting 16 colors plus 2 scatter parameters. The addition of the UV laser will allow for the use of larger fluorescent panels by utilizing the growing family of Brilliant Ultra Violet (BUV) reagents, as well as a variety of Hoechst/DAPI dyes for DNA and cell cycle analysis. We have also purchased the latest version of the industry standard in Cell Cycle Analysis and Flow Cytometry Modeling software, ModFit LT V4.0, which is available for those interested in cell cycle analysis.
For cell sorting a BD FACSAria III is configured with solid state 488nm (blue) and 633nm (red) lasers supporting 7 colors and 2 scatter parameters. The FACSAria III cell sorter is capable of 4-way sterile sorting with available options including automatic cell deposition sorting (sorting into plates), sample and collection temperature control and an aerosol containment unit.
Investigators have the option, once trained, of scheduling and performing their own acquisition and analysis on the LSRFortessa. Fees for use of the LSRFortessa are currently set at $25.00 per hour for University of Louisville investigators. Fees for training are currently set at $100.00 per session (usually two sessions required for beginners) with reduced or no fees with proof of operating expertise.
Cell sorting on the FACSAria III is performed only by the facility’s staff. Fees for cell-sorting are currently set at $45.00 per hour for University of Louisville investigators. A $50.00 setup charge for cell sorting will be added to the hourly fee.
Please contact Robert Miller at firstname.lastname@example.org regarding scheduling or fee information for non-University users.