Qianhong Li, M.D., Ph.D., F.A.H.A.

Associate Professor of Medicine

Department of Medicine, Division of Cardiovascular Medicine

502-852-2463 qhli0001@louisville.edu

Research Interests:

For nearly 18 years, Dr. Li has been investigating the mechanisms responsible for myocardial ischemia / reperfusion injury and developing the cardioprotective strategies including gene therapy and stem cell therapy. Dr. Li’s studies have demonstrated for the first time the effect of extracellular superoxide dismutase gene therapy on conscious rabbits against myocardial stunning and infarction, and the cardioprotective effect of inducible nitric oxide synthase gene therapy against myocardial infarction using a mouse model. She has developed a novel recombinant adeno-associated viral vector (rAAV) system, which is currently accepted as the most encouraging among all viral vector systems for clinical gene therapy. Additionally, she has established and developed a non-invasive, cardiac-specific biomarker system to monitor the extent of cardiac damage resulting from myocardial ischemia/reperfusion injury in vivo using three animal models (mice, rabbits, pigs). Combining the powers of molecular biology and gene transfer with the regenerative capacity of stem cells, Dr. Li and her associates are exploring the potentiation and mechanisms of adult cardiac stem cells to repair damaged myocardium. As a pioneer in this area, Dr. Li has procured NIH funding to create an adult cardiac stem cell system in the mouse. So far, this system has more than 30 cell populations.

Techniques in Use:

  • Molecular Biology- DNA cloning and gene expression, RT-PCR, microRNA profiles, real-time PCR, Western immunoblotting, ELISA, Chromatin immunoprecipitation (ChIP) analysis on gene promoter.
  • Cell Biology - Cell line culture (293, H9C2, COS7, and smooth muscle cells); primary isolation and culture of neonatal cardiac myocytes from rat; isolation, sorting, expansion, and analysis of adult cardiac stem cells.
  • Virology and Gene Transfer- Design, construction, propagation, and analysis of rAd5 and rAAV vectors. Gene delivery mediated by rAd5 or rAAV in vivo (rabbits and mice), or by lipofectamine in vitro.
  • Pathology - Preparation and section of frozen OCT blocks and paraffin blocks, immunohistochemistry and immunofluorescent staining, trichrome staining, and microscope image analysis.

Selected Publications: