Barbara J. Clark, Ph.D.

Associate Professor

Department of Biochemistry and Molecular Biology

319 Abraham Flexner Way, HSC-A, Room 501 502-852-2814 502-852-6222 (fax)

Education, Awards, Service

B.A.,   1981, Zoology, Miami University, Oxford, OH

Ph.D., 1992, Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX

Certificate in Health Professions Education, 2014, University of Louisville.

Senior Editor Board (2011- present) Journal of Endocrinology/Journal of Molecular Endocrinology

Director of Graduate Studies

Laboratory Personnel

No current lab personnel

Research Interests

●          Regulation and function of the START family of lipid/sterol transporters

●          Post-transcriptional regulation of the sodium-phosphate cotransporter type IIa (NPT2a)

●          DHEA regulation of estrogen receptor and androgen receptor activities.

Dr. Clark’s research background is in the area of reproductive endocrinology with work devoted to the identification and characterization of the steroidogenic acute regulatory (StAR) protein in cholesterol transport in steroidogenic tissues. Her group helped define the transcriptional mechanisms that control StAR expression in testicular Leydig cells and the adrenal, providing insight into tropic hormone regulation of steroid hormone output.  StAR is the namesake of the Steroidogenic Acute Regulatory Protein (StAR)-related Lipid Transfer Domain (START) domain protein family.  Members of the mammalian START domain protein family bind and transport lipid ligands and current work in the Clark lab is on defining the role of STARD5 in cholesterol and bile acid metabolism in polarized epithelial cells. We explore mechanisms regulating the expression and function of STARD5 in the kidney, small intestine, and lung.  This research is part of a collaborative effort with faculty at the University of Louisville including Drs. Eleanor Lederer and Syed J. Khundmiri [Department of Medicine, Nephrology Division], Dr. Matt Cave [Department of Medicine, GI Division) and Dr. Carolyn M. Klinge [Biochemistry & Molecular Biology].  Through these collaborations, Dr. Clark has contributed to defining the post-transcriptional mechanisms regulating sodium-phosphate cotransporter type IIa (NPT2a) mRNA stability and protein trafficking in renal proximal tubule cells and adrenal androgen regulation of miRNA expression in hepatocytes.

      Selected Publications

      Y.C. Chen, R. Meier, S.J. Khundmiri, S. Zheng, M.T. Tseng, E.D.Lederer, P.N. Epstein, and B. J. Clark.  2009. Steroidogenic Acute Regulator (StAR)-Related Lipid Transfer Domain Protein 5 (StarD5) Localization in Renal Tubules. Am. J. Physiol. Renal Physiol. 297: F380-F388. PMC2724253.

      S Salyer, M. L. Merchant, N. Lesousky, D.L. Wilkey, E. J. Weinman, B.J. Clark, E.D. Lederer, S.J. Khundmiri. 2011 Dopamine Regulation of Na+-K+ ATPase Requires the PDZ-2 Domain of Sodium Hydrogen Regulatory Factor - 1 (NHERF-1) in Opossum Kidney Cells.  Am. J. Physiol. Cell Physiol. 300(3):C425-34.  PMID: 21160026.

      R.K. Meier & B.J. Clark. 2012. Angiotensin II-dependent transcriptional activation of human Steroidogenic Acute Regulatory Protein gene by a 25-kDa cAMP-Responsive Element Modulator Protein and Yin Yang 1. Endocrinology 153(3):1256-68. PMID: 22253417

      B.J. Clark. 2012. The mammalian START domain protein family in lipid transport in health and disease. J.Endocrinol. 212(3):257-75. PMID: 21965545

      R. Murray, K. Holthouser, B. J. Clark, S.A. Salyer, M.T. Barati, S.J. Khundmiri, and E.D. Lederer 2013. Parathyroid Hormone (PTH) Decreases Sodium-Phosphate Cotransporter Type IIa (NpT2a) mRNA Stability. Am J Physiol Renal Physiol 304(8):F1076-85. PMID: 23466993.

      S.A. Salyer, J.R. Olberding, A.A. Distler, E.D. Lederer, B. J. Clark, N.A. Delamere, and S.J. Khundmiri. 2013. Vacuolar ATPase driven potassium transport in highly metastatic breast cancer cells.  Biochim Biophys Acta 1032(10):1734-43.  PMID 23639630.

      S.A. Salyer, J. Parks, M.T. Barati, E.D. Lederer, B. J. Clark, J.D. Klein, and S.J. Khundmiri. 2013. Aldosterone regulates Na+,K+ATPase activity in human renal proximal tubule cells through mineralocorticoid receptor.  Biochim Biophys Acta 1833(10):2143-52.  PMID 23684706.

      S.J. Khundmiri, S.A. Salyer, B. Farmer, N Qipshidze-Kelm, R.D. Murray, Z. Xie, T.A. Pressley, B. J. Clark, and E.D. Lederer.  2014. Structural determinants for the ouabain-stimulated increase in Na-K ATPase activity. Biochim Biophys Acta 1843(6):1089-102.  PMID 24566089.

      Y. Teng, L.M. Litchfield, M.M. Ivanova, R.A. Prough, B.J.Clark, and C.M. Klinge. Dehydroepiandrosterone-induces miR-21 through estrogen receptor beta and androgen receptor. Mol. Cell. Endocrinol. 2014, ms # MCE-D-14-00024, in press 2014.