William Guido, PhD

William Guido, PhD

William Guido, Ph.D.

Professor and Chair, Department of Anatomical Sciences & Neurobiology

Address: 511 South Floyd, Room 111, Louisville, KY 40202
Phone: (502) 852-5165
Fax: (804) 828-9477
Email


Research Focus

Dr. Guido's research focuses on the form and function of developing sensory systems. Of particular interest is understanding the cellular and molecular mechanisms responsible for the activity dependent refinement of sensory connections. The laboratory's model system has been the mouse lateral geniculate nucleus, the thalamic relay between retina and visual cortex. This pathway develops from a crudely wired, relatively undifferentiated network of cells into a highly ordered sensory system comprised of precise retinotopic patterns of connectivity, separate eye-specific domains, distinct cell types, and elaborate intrinsic circuitry.


Techniques

A variety of in vitro electrophysiological recording techniques are utilized, including visualized whole cell patch recordings. Anatomical experiments involve the use of anterograde tracers, molecular and immunocytochemical markers, and biocytin labeled material to delineate the functional and structural organization of the developing visual system. Biochemical experiments make use of western blots and PCR to examine the molecular composition of neural elements underlying visual system development. More recently, confocal microscopy and optogenetics have been utilized to capture the dynamics of developing thalamic circuitry.


Research

Present research activities include defining the anatomical and functional state of the developing retinogeniculate synapse, understanding the mechanisms that regulate the development of nonretinal circuitry in dLGN, using optogenetics to activate retinal and nonretinal circuits in dLGN, understanding how parallel visual channels from retina are organized in central visual targets.


Key Publications

Kerschensteiner D., Guido W. (2017) Organization of the dorsal lateral geniculate nucleus in the mouse. Visual Neuroscience. 34: E008.

Bickford M.E., Zhou N., Krahe T.E., Govindaiah G., Guido W. (2015) Convergence of "driver-like" inputs in the direction-selective zone of the mouse visual thalamus. Journal of Neuroscience, 35(29):0523-34.

El-Danaf, R., Krahe, T. E., Dilger E.K., Fox, M.A., Guido, W. (2015) Developmental remodeling of relay cells in the dorsal lateral geniculate nucleus in the absence of retinal input. Neural Development, 10:19.

Dilger, E. K., Morhardt, D., Krahe, T. E., Shin, H-S, Guido, W. (2015) Absence of synaptically evoked plateau potentials leads to a breakdown in retinogeniculate refinement. Journal of Neuroscience, 25, 35(8):3652-62.

Seabrook T.A., Krahe T.E., Govindaiah G., Guido W. (2013) Interneurons in the mouse visual thalamus maintain a high degree of retinal convergence throughout postnatal development. Neural Development, 8:24.

Brooks J.M., Su J., Levy C., Wang J.S., Seabrook T.A., Guido W., Fox M.A. (2013) A molecular mechanism regulating the timing of corticogeniculate innervation. Cell Reports, 5(3):573-81.

Seabrook T. A., El-Danaf R. N., Krahe T. E., Fox M. A., Guido W. (2013) Retinal input regulates the timing of corticogeniculate innervation. Journal of Neuroscience, 24:10085-97.

Kuwajima T., Sitko A. A., Bhansali P., Jurgens C., Guido W., Mason C. (2013) ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue. Development, (6):1364-8.

Krahe, T. E., Seabrook, T., Fox, M. A., Chen, C-K, Guido, W. (2012) Modulation of CREB in the dorsal lateral geniculate nucleus of normal and dark reared mice. Neural Plasticity, 426-437.

Krahe, T.E., El-Danaf, R.N., Dilger, E.K., Henderson, S.C. & Guido, W.  (2011) Morphologically distinct classes of relay cells exhibit regional preferences in the dorsal lateral geniculate nucleus of the mouse. Journal of Neuroscience, 31(48) 17437-17448.

Krahe T.E. & Guido W*. (2011). Homeostatic plasticity in the visual thalamus by monocular deprivation.  Journal of Neuroscience, 31(18): 6842-6849. (View PDF)

McNeill D.S., Sheely C.J., Ecker J.L., Badea T.C., Morhardt D., Guido W. & Hattar S. (2011) Development of melanopsin-based irradiance detecting circuitry. Neural Development, 6(1): 8. (View PDF)

Dilger, E. K., Shin, H. S., & Guido, W*. (2011) Requirements for synaptically evoked plateau potentials in relay cells of the dorsal lateral geniculate nucleus of the mouse.  Journal of Physiology, 589 (5) 1103-1115. (View PDF)

Su, J., Haner, C. V., Imbery, T. E., Brooks, J. M., Morhardt, D. R., Gorse, K., Guido W. & Fox M.A. (2011). Reelin is required for class-specific retinogeniculate targeting. Journal of Neuroscience, 31(2), 575-586 9. (View PDF)

Bickford, M. E., Slusarczyk, A., Dilger, E. K., Krahe, T. E., Kucuk, C., & Guido, W. (2010) Synaptic development of the mouse dorsal lateral geniculate nucleus.  Journal of Comparative Neurology, 518(5), 622-635. (View PDF)

Ziburkus, J., Dilger, E. K., Lo, F. S., & Guido, W*. (2009). LTD and LTP at the developing retinogeniculate synapse. Journal of Neurophysiology, 102(6), 3082-3090. (View PDF)

Guido, W. (2008). Refinement of the retinogeniculate pathway. Journal of Physiology, 586, 4357-4362. (View PDF)

Jeon, D., Song, I., Guido, W., Kim, K., Kim, E., Oh, U., et al. (2008). Ablation of Ca2+ channel beta3 subunit leads to enhanced N-methyl-D-aspartate receptor-dependent long term potentiation and improved long term memory.  Journal of Biological Chemistry, 283(18), 12093-12101. (View PDF)

Demas, J., Sagdullaev, B. T., Green, E., Jaubert-Miazza, L., McCall, M. A., Gregg, R. G., Wong R.O. & Guido W. (2006). Failure to maintain eye-specific segregation in nob, a mutant with abnormally patterned retinal activity. Neuron, 50(2), 247-259. (View PDF)

Ziburkus, J., & Guido, W. (2006). Loss of binocular responses and reduced retinal convergence during the period of retinogeniculate axon segregation. Journal of Neurophysiology, 96(5), 2775-2784 (View PDF)

Jaubert-Miazza, L., Green, E., Lo, F. S., Bui, K., Mills, J., & Guido, W. (2005). Structural and functional composition of the developing retinogeniculate pathway in the mouse. Visual Neuroscience, 22(5), 661-676. (View PDF)

Ziburkus, J., Lo, F. S., & Guido, W. (2003). Nature of inhibitory postsynaptic activity in developing relay cells of the lateral geniculate nucleus. Journal of Neurophysiology, 90(2), 1063-1070. (View PDF)

Li, J., Bickford, M. E., & Guido, W. (2003). Distinct firing properties of higher order thalamic relay neurons. Journal of Neurophysiology, 90(1), 291-299. (View PDF)

Lo, F. S., Ziburkus, J., & Guido, W. (2002). Synaptic mechanisms regulating the activation of a Ca2+ mediated plateau potential in developing relay cells of the LGN. Journal of Neurophysiology, 87(3), 1175-1185. (View PDF)

Weyand, T. G., Boudreaux, M., & Guido, W. (2001). Burst and tonic response modes in thalamic neurons during sleep and wakefulness. Journal of Neurophysiology, 85(3), 1107-1118. (View PDF)