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Core B - Surgery & Animal Care

Location:





MDR 603 Core Animal Surgery


MDR 604 Animal Pre/Post-Op


MDR 613 Microinjection/Virus injection





Core Director: Theo Hagg, M.D., Ph.D. 
Personnel: Christine Yarberry


Kim Cash

The facility contains a prep room and 8 general operating stations each with a surgical microscope.  Also available are a dedicated picospritzer setup for microinjections and a BSL2 biosafety hood for injection of viruses.  This Core is managed daily by highly skilled personnel and is overseen by a leading expert in various in vivo models.

Users provide their own surgeons who are trained one-on-one by Core personnel, if necessary. 

This Core has 10 years of experience serving KSCIRC combined with a background of innovative surgical approaches. Central to the success is the state-of-the-art but standardized surgical and animal care approaches, integrated with functional analyses of Core C.

The facility contains a prep room and 8 general operating stations each with a surgical microscope.

    The following procedures are available for training or assistance:
  • spinal cord contusion injuries with the IH, LISA and NYU impactor devices
  • stereotaxic partial or complete cord transections with the vibraknife (LISA)
  • excitotoxic or ischemic lesions
  • demyelinating lesions
  • microinjections of cells or pharmacological agents
  • injections of viruses from Core B to deliver genes in a dedicated BL2 biosafety hood using a remote controlled stereotaxic apparatus
  • implantation of chronic Alzet pumps
  • implantation of EMG recording electrodes
  • repeated jugular or tail vein injections
  • microchipping for identification

 

Note that the functional outcomes of the injuries have been documented by the Behavioral  and Electrophysiological Assessment Core, serving as reference points for future studies.  

The Core is managed daily by a highly skilled surgeon and teacher, Christine Yarberry.  Training in animal care is provided by Kim Cash, who has vast experience and success in postoperative care.  The overall operations will be overseen by Core Director, Dr. Theo Hagg who is a leading expert in various in vivo models.  

Users should contact Ms. Yarberry (email Ms. Yarberry) to schedule use of the facility or to discuss their training needs.  Please be prepared to show proof of UofL IACUC and, if necessary, Institutional Biosafety Committee approval.  Level II animal training and registration on the IACUC protocol is required for all personnel and users.

If projects are not funded by peer-reviewed grants, Users are required to submit a 1 page specifics aims page to the Core B steering committee (email the Steering Committee) who will evaluate whether research projects are scientifically qualified for Core use.  

For advice and assistance for developing novel surgical approaches please contact Dr. Hagg (email Dr. Hagg).

 

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Figure 1.  Eight general surgical tables each with a surgical microscope are available to users.

 

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Figure 2.  Three impactor devices are available, from left to right, IH, LISA and NUY impactors.

 

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Figure 3. Stereotaxic injections of cells or reagents can be delivered in the nano- or microliter range using our picospritzer unit.

 

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Figure 4.  Injections of viruses to deliver genes are performed in a dedicated BL2 biosafety hood using a remote controlled stereotaxic apparatus

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