Email Kyung Kang
Phone Number: (502) 852-2094
Fax Number: (502) 852-6355
Address: Department of Chemical Engineering

Website:  https://louisville.edu/speed/faculty/kang

Speed School of Engineering
Ernst Hall 312 (Office)
Ernst Hall 303  A&B( Lab)
University of Louisville
Louisville, KY 40292
Ph. D. 1991, University of California, Davis

Investigators:

•  Jianting Wang, Ph.D. Candidate
• Robert Lupitskyy, Postdoctoral Fellow
• Michell Liu - Manual High School Student
• Sarah Y Wang Manual High School Student

Research Interests:

• Nano-metal particles for Fluorescence Enhancement
• Nanoparticles for Optical Contrast in Optical Mammography
• Application of Nano-magnetic Particles for Tumor Specific Hyperthermia

• Biosensing systems for disease diaganosis/monitoring/prognosis – Anticoagulants (Protein C, Protein S, Antithrombin III, Plasminogen), Factor V Leiden), Cardiac Markers (Cardiac Troponin T/I, CRP, BNP, Myoglobin)

• NIR Spectroscopy (Experimental, Clinical, and Theoretical):  Algorithm Development for Absolute Optical Parameters; Characterization of Oxygen Saturation State in various Tissue; Frequency Response Analysis Theory for Optical Pulse Testing; Algorithm Development for Clinical Imaging

• Application: Oxygen Saturation Measurement; Breast Tumor Localization; Hemotoma Detection; Blood Flow Measurement; Deep Vein Thrombosis Detection

• Multi-Spectral Image Analysis for Skin Cancer Detection.

•  Ion Exchange Chromatography; Various Affinity Chromatography Separation; Purification of Protein C (Anti-coagulant) from Blood Plasma.

• Co-Developer of a probabilistic/numerical technique, the B-W-K Technique.
• Oxygen Transport to Tissue
• Heat Transfer during Cancer Hyperthermia
• Photon Transfer in highly scattering media

Awards:

•  IICT-Avon Padmashri Dr G S Sidhu Chemcon Distinguished Speaker Award, “Application of Nanoparticles for Disease Diagnosis and Treatment,” CHEMCON 2009, December 27-30, Visakhapatnam, India.
• University Scholar of University of Louisville, since March 22, 2001

• NSF CAREER Award recipient, 1998.

• The Most Outstanding Research Paper Published in 1997 - for the paper, "Feasibility Study of a Single and Multiple Source NIR Phase Modulation Device for Biological System Characterization," Biomedical Instrumentation & Technology - May 31, 1998.

• MELVIN H. KNISELY AWARD [Young Investigator's Award - International Society on Oxygen Transport to Tissue (ISOTT); 1988]

Selected Publications:

1. Wang, J., Nantz, M.H., Achilefu, S., and Kang, K.A., “FRET-Like Fluorophore-Nanoparticle Complex for Highly Specific Cancer Localization,” Oxygen Transport to Tissue XXXI, Advances in Experimental Medicine and Biology, Vol. 662, Takahashi, E. and Bruley, D.F. (Eds.) P. 407-414, 2010.
2. Wang, J., Nantz, M.H., Achilefu, S. and Kang, K.A., FRET-Like Fluorophore-Nanoparticle Complex for Highly Specific Cancer Localization,” Proceeding of 2008 Annual ISOTT Conference, August 3-7, 2008, Sapporo, Japan.
3. Kang, K.A., Hong, B., and Jin, H., “Nano Metal Particles for Biomedical Applications,” Book Chapter in "Nanoscience in Biomedicine," Donglu Shi (ed.) Springer-Verlag and Tsinghua University Press, Chapter 12, p.290-313, 2009.
   
4. Kang, K.A., Ren, Y., Sharma, V.R., and Peiper S.C., “Near Real-time Immuno-optical Sensor for Diagnosing Single Point Mutation (A model System: Sensor for Factor V Leiden Diagnosis), Biosensor and Bioelectronics, 24 (2009) 2785–2790.
5. Wang,J., Hong, B., Kai, J., Han, J., Zou. Z., Ahn, C.H., and Kang, K.A., “Mini sensing chip for Point-of-Care AMI diagnosis utilizing MEMS and nano-technology,” Advances in Experimental Medicine and Biology – Oxygen Transport to Tissue XXX (eds. P. Liss, P. Hansell, D.F. Bruley, and D. K. Harrison) Vol. 645, pp.101-108, 2009.
6. BOOK – Kang, K. A.; Harrison, D. K.; Bruley, D. F., Oxygen Transport to Tissue XXIX: Advances in Experimental Medicine and Biology, Vol. 614, January 2008.
7. Kakar, S, Jin, H., Hong, B., Eaton, J.W.,  and Kang, K.A. “LHRH receptor targeted therapy for breast cancer,” Oxygen Transport to Tissue XXIX; Advances in Experimental Medicine and Biology, Vol. 614, Kang, K.A.; Harrison, D. K.; Bruley, D. F. (Eds.), p.285-296,.2008
8. Lee, J.J., Bruley, D.F., and Kang, K.A., “Manipulation of the Affinity between Protein and Metal Ions by Imidazole and pH for Metal Affinity Purification of Protein C from Cohn Fraction IV-1,” Oxygen Transport to Tissue XXIX; Series: Advances in Experimental Medicine and Biology, Vol. 614, Kang, K.A. ; Harrison, D. K.; Bruley, D. F. (Eds.), p.93-100, 2008
9. Ren, Y., Rezania, S., and Kang, K.A., “Biosensor for Diagnosing Factor V Leiden, a Single Amino Acid Mutated Abnormality of Factor V,” Oxygen Transport to Tissue XXIX; Series: Advances in Experimental Medicine and Biology, Vol. 614, Kang, K.A.; Harrison, D. K.; Bruley, D. F. (Eds.), p.245-252, 2008
10. Rezania, S. and Kang, K.A., “Separation of Factor V Leiden Molecule, A Mutated Form of Factor V, from Plasma of Homozygous Patient,” Oxygen Transport to Tissue XXIX; Series: Advances in Experimental Medicine and Biology, Vol. 614, Kang, K.A.; Harrison, D. K.; Bruley, D/ F. (Eds.), p. 101-107, 2008
11. Hong, B., Kai, J., Ren, Y., Han, J., Zou, J. Ahn, C.H., and Kang, K.A., “Highly Sensitive, Rapid, Reliable, and Automatic, Cardiovascular Disease Diagnosis with Nanoprticle Fluorescence Enhancer and MEMS,” Oxygen Transport to Tissue XXIX; Series: Advances in Experimental Medicine and Biology, Vol. 614, Kang, K.A.; Harrison, D. K.; Bruley, D. F. (Eds.), p. 265-273, 2008
12. Jin, H, Hong, B., Kakar, S.S., and Kang, K.A., “Tumor Specific Nano-entities for Optical Detection and Hyperthermic Treatment of Breast Cancer,” Oxygen Transport to Tissue XXIX; Series: Advances in Experimental Medicine and Biology, Vol. 614 Kang, K.A.; Harrison, D. K.; Bruley, D. F. (Eds.), p. 275-284, 2008
13. Jin, H. and Kang, K.A., “Application of Novel Metal Nanoparticles as Optical/Thermal Agents in Optical Mammography and Hyperthermic Treatment for Breast Cancer,” Adv Exp Med Biol., 599, Oxygen Transport to Tissue XXVIII (Maguire, D.J., Bruley, D.F., Harrison, D.K., eds.), p.45-52, 2007.
14. Rezania, S., Ahn, D.K., and Kang, K.A., “Separation of Protein C from Cohn Plasma Fraction IV-1 by Mini Antibody,” Adv Exp Med Biol., 599, Oxygen Transport to Tissue XXVIII (Maguire, D.J., Bruley, D.F., Harrison, D.K., eds.), p.125-132, 2007.
15. Lee, J.J., Bruley, D.F., and Kang, K.A., “Effect of pH and Immidazol on Protein C Purification by Immobilized Metal Affinity Chromatography from Cohn Fraction IV-1," Adv Exp Med Biol., 599, Oxygen Transport to Tissue XXVIII (Maguire, D.J., Bruley, D.F., Harrison, D.K., eds.), p. 53-60, 2007.
16. Hong, B.,Tang, L., Ren, Y.J., and Kang, K.A., "Real-time, Automated, Fluorophore Mediated Multi-Cardiac Marker Biosensing System With Nano-metallic Particle Reagent," Adv Exp Med Biol., 599, Oxygen Transport to Tissue XXVIII (Maguire, D.J., Bruley, D.F., Harrison, D.K., eds.), p. 23-30, 2007.
17. Kai  J., Zou, Z., Han, J., Lee, L., Hong, B., Ren, Y., Kang, K.A.,  and Ahn, C.A., “ Automated Fluidic System with  a Chaotic Microfluidic Reaction Chamber for Rapid, Multi-Analyate Immuno-Sensing,” Proceeding of the Annual Conference of the 14th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers'07), Lyon, France, June 10-14, 2007, pp. 735-738, 2007.
18. Lee, S., Hong, B., Kang, K.A. and Ahn, C.H., “A Disposable Optical Polymer Lab-On-A Chip With Injection-Molded Polymer Waveguides For Measuring Protein-C,” Proceedings Of The 9th World Congress On Biosensors (Biosensors 2006), Toronto, Canada, O81, May 10-12, 2006.
19. Tang, L. and Kang, K.A., “Preliminary study of fiber optic multi-cardiac-marker biosensing system for rapid coronary heart disease diagnosis and prognosis,” Adv Exp Med Biol., 578, Oxygen Transport to Tissue XXVII, p. 101-106, 2006.
20. Rezania, S., Ahn D.G., and Kang, K.A., “Cost Effective Metal Affinity Chromatography for Protein C Specific Mini-Antibody Purification,” Adv Exp Med Biol., 578, Oxygen Transport to Tissue XXVII, p. 49-54, 2006.
21. Hong, B. and Kang, K.A., “Fluorescence Enhancers for Fluorophore Mediated Biosensors for Cardiovascular Disease Diagnosis,” Adv Exp Med Biol., 578, Oxygen Transport to Tissue XXVII, p. 179-184, 2006.
22. Tang, L., Ren, Y.J., Hong, B. and Kang, K.A., "A Fluorophore-mediated, Fiber-optic, Multi-analyte, Immuno-sensing System for Rapid Diagnosis and Prognosis of Cardiovascular Diseases." Journal of Biomedical Optics, 11(2), 021011(p.1-10), March/April 2006
23. INVITED PAPER, Kang, K.A. and Hong, B., “Biocompatible Nano-Metal Particle Fluorescence Enhancers,” Critical ReviewsTM in Eukaryotic Gene Expression, 16(1): p. 45-60, 2006.
24. Hong, B. and Kang, K.A., “Biosensor compatible, nanogold particle fluorescence enhancer for optical immunoassay,” Biosensor and Bioelectronics, 21, p. 1333~1338, 2006.
25. Tang, L. and Kang, K.A., “Preliminary study of simultaneous multi-anticoagulant deficiency diagnosis by fiber optic multi-analyte biosensor,” Advances in Experimental Medicine and Biology, 566, Oxygen Transport to Tissue XXVI, Okunieff, P., Williams, J., Chen, Y. (Eds.), p. 303-310, Springer, 2005.
26. Jin, H., and Kang, K.A., “Fluorescent mediated detection of heterogeneity in a highly scattering media,” Advances in Experimental Medicine and Biology, 566, Oxygen Transport to Tissue XXVI Okunieff, P., Williams, J., Chen, Y. (Eds.), p.167-172, Springer, 2005.

Patents & Licenses:

•  09013 -- Multifunctional Nanoparticles for Use as Contrast Agents for Multi-Imaging Modalities and as Therapeutics; Research Disclosure submitted on July 30, 2008.

• 08082 – Conjugated Gold Nanoparticles for Targeted Cancer Treatment; PENDING: Priority Filing: Provisional patent application no. 61/073,635; filed June 18, 2008.

• 07074 – Cancer Site Specific Fluorescence Marking and Contrast Marker for Same; Priority Filing: Provisional patent application no. 61/072,427; filed March 31, 2008. PENDING: PCT/US2009/TBA; Cancer Site Specific Fluorescence Marking and Contrast Marker for Same; filed March 31, 2009.
  

• 07071 – A New Method to Induce the Heat Shock Response; Research Disclosure submitted on June 21, 2007.

•  07019 – Transdermal Application of Cancer-Specific Nanoparticles for Cancer Treatment; Research Disclosure submitted on September 13, 2006.

• 06052 – Protein C and Other High Molecular Weight Therapeutic Protein Purification by Immobilized Metal Affinity Chromatography from Complex Source Material; Research Disclosure submitted April 4, 2006.

• 05030 – Cancer Specific Nanoparticles for Optical Contrast Enhancement, and Local, Inductive Hyperthermic Treatment; Priority Filing: Provisional patent application no. 60/663,777, filed March 21, 2005. PUBLISHED; decided not to further pursue: Target Specific Nanoparticles for Enhancing Optical Contrast Enhancement and Inducing Target-Specific Hyperthermia; Patent Corporation Treaty application no. PCT/US06/010191, filed March 21, 2006.
  

• 04063 – Fiber-Optic Multi-Cardiac-Marker Fluorophore Mediated Biosensing and Bioimaging; Priority Filing: Provisional patent application no. 60/653,543, filed February 16, 2005.
• PCT:  Fiber-Optic Biosensor and Biosensing Methods: Patent Corporation Treaty application no.  PCT/US2006/005376, filed February 16, 2006.
  
• PENDING: Fiber Optic Multi-Cardiac Marker Biosensing System for Rapid Coronary Heart Disease Diagnosis; Non-provisional patent application no. 11/355,467, filed February 16, 2006.
  

• 04034 – Optimized Production Process for the Mini-Antibody Against Protein C Using E. Coli; Research Disclosure submitted January 12, 2004.

• 04033 – Economic Process for Purification of Single Chain Variable Fragments (Mini-Antibody) by Immobilized Metal Affinity Chromatography (IMAC); Research Disclosure submitted January 12, 2004.

• 04032 – Economic Process for Purification of Protein C Using the Single Chain Variable Fragment (Mini-Antibody); Research Disclosure submitted January 12, 2004.

• 04031 – Near Real Time Biosensor for the Factor V Leiden Diagnosis. PENDING: Non-provisional patent application no. 11/891,452 filed August 10, 2007; Status:  April 2009: Received notice of publication; June 2009: Status inquiry with the USPTO; Waiting for first Office Action.
  

• 04030 – Multi  (More Than 2) -Anticoagulant Deficiency Detection System (Simultaneous Detection System); Research Disclosure submitted December 17, 2003.

• 04029 – Real Time or Near-Real Time Sensor for Protein C Deficiency Diagnosis (Specifically, Application of Convective Flow, an Optimization Component); Research Disclosure submitted  December 17, 2003.

• 04027 – Enhancement of Sensitivity of Fluorophore Mediated Biosensing Applications: Priority Filing: Provisional patent application no. 60/590,992, filed July 26, 2004.

• 04028 – Disclosed: Reagent for Enhancement of Fluorescence (filed together with 04027).
  
• PENDING:  Enhancement of Sensitivity of Fluorophore Mediated Biosensing and Bioimaging: Non-Provisional patent application no. 11/190,199, filed July 26, 2005.  Patent granted October, 2010.
  
•  PENDING:  Enhancement of Sensitivity of Fluorophore Mediated Biosensing and Bioimaging: Divisional patent application no. 11/394,290, filed March 30, 2003. Patent granted October, 2010.