Chuan Hu, Ph.D.
Assistant Professor of Biochemistry & Molecular Biology
Email Chuan Hu
Phone Number: (502) 852-6287 (Office)
Fax Number: (502) 852-6222
Address: 319 Abraham Flexner Way, Rm 515
Dept. of Biochemistry & Molecular Biology
University of Louisville School of Medicine
Louisville, KY 40202
Ph. D. 2000, Columbia University Medical Center
- David Humphrey, Research Technician
Research Interests:Vesicular Trafficking in Cancer Metastasis and Angiogenesis:
Metastasis, the spreading of cancer from a primary site to distant organs, is responsible for 90 percent of the deaths from cancer. However, cancer metastasis is not well understood at the molecular level. The research of the laboratory is focused on a fundamental event of metastasis – migration of cancer cells. In migrating cells, plasma membrane components and the cell adhesion receptors integrins are transported by vesicles and exocytosed at the cell front. Using a variety of techniques including molecular biology, cell biology and confocal imaging, we are characterizing the SNARE protein complexes that mediate polarized exocytosis in cell migration. Our goal is to elucidate how SNARE proteins interact with signal transduction cascades to modulate the migration and metastasis of cancer cells.
Another project is aimed at VE-cadherin, the primary component of adherens junctions in endothelial cells. VE-cadherin regulates endothelial barrier function and the organization of endothelial cells during angiogenesis. We are investigating how vesicular trafficking regulates the availability and function of VE-cadherin on the cell surface.
- Riggs, K.A., Hasan, N., Humphrey, D., Read, C., Nevitt, C., Corbin, D., and Hu, C. (2012). Regulation of integrin endocytic recycling and chemotactic cell migration by syntaxin 6 and VAMP3 interaction. J. Cell Sci. 125 (pt 16):3827-3839.
- Hasan, N., Humphrey, D., Riggs, K.A., and Hu, C. (2012). Analysis of SNARE-mediated membrane fusion using an enzymatic cell fusion assay. J. Vis. Exp. Oct 19;(68).
- Teng, Y., Manavalan, T.T., Hu, C., Medjakovic, S., Jungbauer, A., and Klinge, C.M. (2013). Endocrine Disruptors Fludioxonil and Fenhexamid Stimulate miR-21 Expression in Breast Cancer Cells. Toxicol. Sci. 131(1):71-83.
- Day, P., Riggs, K.A., Hasan, N., Corbin, D., Humphrey, D., and Hu, C. (2011). Syntaxins 3 and 4 mediate vesicular trafficking of a5b1 and a3b1 integrins and cancer cell migration. Int. J. Oncol. 39:863-871.
- Imbert-Fernandez, Y., Radde, B. N., Teng, Y., Young, W.W., Hu, C., and Klinge, C.M. (2011). MUC1/A and MUC1/B splice variants differentially regulate inflammatory cytokine expression. Exp. Eye Res. 93(5):649-657.
- Hasan, N., Corbin, D., and Hu, C. (2010). Fusogenic pairings of vesicle-associated membrane proteins (VAMPs) and plasma membrane t-SNAREs – VAMP5 as the exception. PLoS One 5(12):e14238.
- Hasan, N., and Hu, C. (2010). Vesicle-associated membrane protein 2 mediates trafficking of a5b1 integrin to the plasma membrane. Exp. Cell Res. 316:12-23.
- Luftman, K., Hasan, N., Day, P., Hardee, D., and Hu, C. (2009). Silencing of VAMP3 inhibits cell migration and integrin-mediated adhesion. Biochem. Biophys. Res. Commun. 380:65-70.
- Hu, C., Hasan, N., and Riggs, K.A. (2012). Analysis of SNARE-mediated exocytosis using a cell fusion assay. In Crosstalk and Integration of Membrane Trafficking Pathways. R. Weigert, editor: InTech. pp. 229-245.
- Hu, C., Hasan, N., and Riggs, K.A. (2012). Vesicular trafficking of the fibronectin receptor 51 integrin. In Fibronectin: Current Concepts in Structure, Function and Pathology. J. Beattie, editor: Nova Science Publishers, Inc. pp.125-140.