Research Methods in Pharmacology and Toxicology 2006-2007
Research Methods in Pharmacology and Toxicology 2006-2007
This team-taught set of courses will provide a brief introduction to the wide range of methods used to conduct research in pharmacology and toxicology. The student will be exposed to departmental experts covering each topic. The course will consist of four 1 credit Modules. Sessions will be held on Tuesdays and Thursdays, 2:00 – 3:00 PM, in the PKK Conference Room (HSCA 1302). 1 h per session, 15 h per module; Module I will be lecture based and grades assigned based on performance on assignments and/or examinations. Modules II –IV will consist of 1 h of lecture followed by 1 h of student presentations of research papers demonstrating the method. Presentations will use a team approach with one member of the team assigned to present and the other members responsible for leading discussion with questions. Grades for modules II-IVwill be assigned based on faculty evaluations of the presentations and discussions.
PHTX 672 Research Methods in Pharmacology and Toxicology - I Basic techniques and fundamentals of laboratory practice |
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8/22/06 |
A. Use of common lab instruments (pH meter and centrifuges) (balance and pipets) |
Benz/Nerland |
8/24/06 |
B. Radioisotope tracers |
Pierce |
8/29/06 |
C. Chromatography |
Hurst |
8/31/06 |
D. Spectrophotometry |
Benz |
9/5/06 |
E. Experimental design and hypothesis testing; Lab notebooks, precision, accuracy, calibration and quality control |
Arteel |
9/7, 9/12, 9/14, 9/19, 9/21, 9/26, 9/28, 10/3, 10/5, 10/12/06 |
F. Introduction to Statistics (10 h) |
Kidd |
PHTX 673 Research Methods in Pharmacology and Toxicology – II Cellular & Molecular Approaches |
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10/17, 10/19/06 |
A. Receptors & signal transduction |
Song |
10/24, 10/26/06 |
B. Transcriptional regulation (reporter assays, gel shifts, etc.) |
E. Gozal |
10/31, 11/2/06 |
C. Detection of gene expression |
Nerland |
11/7, 11/9/06 |
D. RNA regulation |
Wang |
11/14, 11/16/06 |
E. Array analyses |
Zacharias |
11/21, 11/28/06 |
F. Genotoxicity I: DNA damage & repair |
States |
11/30, 12/5/06 |
G. Genotoxicity II: mutagenesis |
McGregor |
12/12/06 |
Capstone lecture: DNA repair defects and premature aging |
Niedernhofer |
PHTX 674 Research Methods in Pharmacology and Toxicology - III Specialized / Advanced Biochemical & Physical Chemical Approaches |
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1/9, 1/11/07 |
A. Biomarkers |
Myers |
1/16, 1/18/07 |
B. Mass spectrometry |
Hurst |
1/23, 1/24/07 |
C. Imaging (fluorescence) |
Pisano |
1/30, 2/1/07 |
D. Proteomics |
Pierce |
2/6, 2/8/07 |
E. Atomic spectroscopy |
Pierce |
2/13, 2/15/07 |
F. Metabolomics |
Fan |
2/20, 2/22/07 |
G. Autoradiography, Phosphorimaging analysis and densitometry |
States |
2/22/07 |
Capstone lecture: topic tba |
Palmer |
PHTX 675 Research Methods in Pharmacology and Toxicology - IV Whole animal and organ system approaches |
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2/27, 3/1/07 |
A.Brain slices |
Schurr |
3/6, 3/8/07 |
B. Perfused organs |
Williams |
3/20, 3/22/07 |
C. ADME study designs |
Chen |
Genetic Approaches |
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3/27, 3/29/07 |
A. Genetically manipulated models I |
Epstein |
4/3, 4/5/07 |
B. Genetically manipulated models II |
Arteel |
4/10, 4/12/07 |
C. Genetic screening |
Zundel |
4/17, 4/19/07 |
D. Ectopic expression in cultured cells |
Zundel |
4/24/07 |
Capstone lecture: Experimental models (from whole animals to molecular) |
Arteel |
