Partha Mukhopadhyay, PhD

Associate Professor

Department of Surgical and Hospital Dentistry


Division of Craniofacial Development & Anomalies Partha.Mukhopadhyay@louisville.edu

Scholarly Activities

Dr. Mukhopadhyay’s principal research focus involves investigation of the role and interaction of various peptide growth factors and transcriptional regulators, whose collective interplay can regulate neural tube and neural crest development, midfacial ontogenesis and palatogenesis. His specific research interests are to examine the role of the nuclear transcriptional regulators, coactivators and corepressors in craniofacial growth and anomalies, to analyze the role of growth factors like TGFßs and BMPs in orofacial development as well as characterization of the various transcription factors and their role in normal orofacial growth. He has used DNA microarray technology to establish comprehensive “mRNA and microRNA expression profiles” of developing murine orofacial tissue and of developing neural tube to identify important candidate microRNAs and their targets - regulating palatogenesis and neural tube development, respectively. His current research projects for which he was awarded NIH funding investigate, employing a combined bioinformatic and molecular approach: (1) the interaction between differentially-expressed miRNAs and their target mRNAs, as well as to define the role of those miRNAs in regulating key processes required for normal orofacial and neural tube development (2) the epigenetically-regulated, differentially expressed candidate genes central to normal orofacial development and also, to identify and characterize the differentially-methylated genomic regulatory elements governing the expression of such candidate genes. His additional research projects investigate: (1) Developmental neurotoxicity of prenatal arsenic and environmental tobacco smoke exposure (2) Epigenetics and Fetal Alcohol Syndrome (3) Developmental toxicity (teratogenicity) of Azacytidine - an inhibitor of DNA methyl transferases.

 

 

Peer-Reviewed Publications (2014-2017):

  1. Warner DR, Mukhopadhyay P, Brock G, Webb CL, Michele Pisano M, Greene RM. MicroRNA expression profiling of the developing murine upper lip. Dev Growth Differ. 2014 Aug;56(6):434-447. PMCID: PMC4379120.
     
  2.  Seelan RS, Mukhopadhyay P, Warner DR, Appana SN, Brock GN, Pisano MM, Greene RM. Methylated microRNA genes of the developing murine palate. MicroRNA. 2014;3(3):160-173. PMCID: PMC4739625.
     
  3.  Warner D, Ding J, Mukhopadhyay P, Brock G, Smolenkova IA, Seelan RS, Webb CL, Wittliff JL, Greene RM, Pisano MM. Temporal Expression of miRNAs in Laser Capture Microdissected Palate Medial Edge Epithelium from Tgfβ3(-/-) Mouse Fetuses. MicroRNA. 2015;4(1):64-71. PubMed PMID: 26159804.
     
  4. Mukhopadhyay P, Greene RM, Pisano MM. Cigarette smoke induces proteasomal-mediated degradation of DNA methyltransferases and methyl CpG-/CpG domain-binding proteins in embryonic orofacial cells. Reprod Toxicol. 2015 Dec;58:140-148. PMCID: PMC4690750.
     
  5. Mukhopadhyay P, Seelan RS, Rezzoug F, Warner DR, Smolenkova IA, Brock G, Pisano MM, Greene RM. Determinants of orofacial clefting I: Effects of 5-Aza-2'-deoxycytidine on cellular processes and gene expression during development of the first branchial arch. Reprod Toxicol. 2017 Jan;67:85-99. PMCID: PMC5303154.
     
  6. Seelan RS, Mukhopadhyay P, Warner DR, Smolenkova IA, Pisano MM, Greene RM. Determinants of orofacial clefting II: Effects of 5-Aza-2'-deoxycytidine on gene methylation during development of the first branchial arch. Reprod Toxicol. 2017 Jan;67:100-110. PMCID: PMC5303139.