Molecular, Cellular & Craniofacial Biology

Molecular, Cellular & Craniofacial Biology

Department of Molecular, Cellular & Craniofacial Biology

dnaWelcome to the Department of Molecular, Cellular and Craniofacial Biology in the School of Dentistry.  The core of the University's mission is the discovery, dissemination, and application of knowledge.  Faculty in university departments, centers and institutes have been classically thought of as the principal incubators of new discoveries and ideas.  To that end, our department has designated the discovery of new knowledge by the performance of scientific research as its primary mission.

Prestige and the respect of one's professional peers is the coin of the realm.  In academia, this is more often than not acquired by recognition of one's research achievements.  As the Department of Molecular, Cellular and Craniofacial Biology evolves, the objective of new faculty recruitment to the department will be to strengthen the developing research focus on molecular/molecular genetic mechanisms of birth defects, specifically, craniofacial anomalies.  This research strength will contribute to, and synergize with, the significant extant focus on children’s disorders in the department, as well as at the university.  Thus, the broad primary departmental objective will be to conduct fundamental and applied research on cellular and molecular aspects of embryonic development and acquired congenital malformations.

“There is something fascinating about science. One gets such wholesale returns of conjecture out of such a trifling investment of facts.”
—Mark Twain - Life on the Mississippi

Interactions between scholars and students enlivens the pursuit of knowledge.  The obligation of each faculty scholar is to work in the context of communicating his/her research to students and trainees.  The university’s research and teaching mission (the creation and dissemination of knowledge) are not separate functions that compete for attention but, rather, are integrated activities.  Thus, an important departmental objective is faculty engagement in exciting teaching—specifically, dissemination of new knowledge acquired through biomedical research.

Faculty and administration are not antithetical entities, despite the erroneous perception of many faculty that administration attempts to save faculty the trouble of thinking for themselves.  Indeed, faculty have multiple academic and scholarly talents and several means of contributing to the health and vitality of a university.  All efficiently functioning academic organizations rely on faculty wisdom and experience to make informed decisions regarding governance.  Thus, each faculty member is expected to contribute to university governance by membership on, and active contribution to, appropriate university committees.

Any simple problem can be made insoluble if enough committee meetings are held to discuss it.
—Anonymous

We hope you take the time to explore links to "Faculty" where details of faculty research activities are defined, and "Publications" where recent faculty publications are listed; and our University Birth Defects Center.

 

Robert M. Greene, Ph.D.

Staff

Support Staff

 

  • Barr, Nancy A. - Business Manager

  • Cheng, Yu - Research Tech./TMP

  • Hadel, Diana - Research Tech. II

  • Smith, Scott - Post Doctoral Fellow

  • Smolenkova, Irina - Research Associate, Sr.

  • Webb, Cynthia L. - Laboratory Research Tech. III

Publications

Recent Peer-reviewed Publications

 Jixiang Ding, Ph.D.

  • Jin JZ, Tan M and Ding J. (2013) Analysis of Cripto expression during mouse cardiac myocyte differentiation. Int J Dev Biol, Oct 30(PMID: 24307303)
  • Ji R, Tian S, Lu HJ, Lu Q, Zheng Y, Wang X, Ding J, Li Q and Lu Q. (2013) TAM Receptors Affect Adult Brain Neurogenesis by Negative Regulation of Microglial Cell Activation.  J Immunol. 191, 6165-77. (PMID: 24244024).
  • Jin JZ, Warner DR, Lu Q, Pisano MM, Greene RM, Ding J (2014) Deciphering TGF-β3 function in medial edge epithelium specification and fusion during mouse secondary palate development. Dev Dyn. (PMID: 25104574)

 

Robert M. Greene, Ph.D.

  • Seelan RS, Mukhopadhyay P, Pisano MM, Greene RM. Developmental Epigenetics of the Murine Secondary Palate. ILAR Journal, special issue “Epigenetics – From Mice to Men.” 53:240-252 (2012). PMID: 23744964 
  • Mukhopadhyay P, Singh S, Greene RM, Pisano MM. Strain-specific modifier genes governing craniofacial phenotypes.  Birth Defects Res A. 94:162-175 (2012). PMCID: PMC3302963 
  • Warner D, Mukhopadhyay P, Webb CL, Greene RM, Pisano MM. Chromati Immunoprecipitation-promoter microarray identification of genes regulated by PRDM16 in murine embryonic palate mesenchymal cells – Exper. Biol. & Medicine 237:387-394 (2012). PMID: 22522345 
  • Warner D, Wells J, Greene RM, Pisano MM. Gene expression changes in the secondary palate and mandible of Prdm16-/- mice. Cell & Tissue Research 351: 445-452 (2013). PMCID: PMC3584240 
  • Amos-Kroohs RM, Williams MT, Vorhees CV, Braun AA, Graham DL, Webb CL, Birtles TS, Greene RM, Pisano MM. Behavioral pheotype of C57BL/6J mice prenatally and neonatally exposed to cigarette smoke. Neurotoxicology & Teratology 35:34-45 (2013). PMCID: PMC3593942
  • Mukhopadhyay P, Rezzoug F, Kaikaus J, Pisano MM and Greene RM. Alcohol modulates expression of DNA methyltranferases and methyl CpG-CpG domain-binding proteins in murine embryonic fibroblasts. Reprod Tox 37:40-48 (2013). PMID: 23395981
  • Brock G, Mukhopadhyay P, Pihur V, Webb C, Greene RM, Pisano MM. MmPalateMiRNA, an R Package Compendium Illustrating Analysis of miRNA Microarray Data. Source Code for Biology and Medicine 8(1): http://www.scfbm.org/content/8/1 - (2013). PMID: 23298515.
  • Seelan RS, Mukhopadhyay P, Warner DR, Pisano MM, Greene RM. Epigenetic regulation of Sox4 during palate development. Epigenomics 5:131-146 (2013). PMID: 2356609
  • Seelan R, Appana SN, Mukhopadhyay P, Warner DR, Brock GN, Pisano MM, Greene RM. Developmental profiles of the murine palatal methylome. Birth Defects Research A  97:171-186 (2013). PMID: 23554260
  • Seelan RS, Warner DR, Mukhopadhyay PM, Andres SA, Smolenkova I, Wittliff JL, Pisano MM, Greene RM.  Epigenetic analysis of laser capture microdissected fetal epithelia. Analytical Biochem 442:68-74 (2013) PMID 23911529.
  • Greene RM and Kirschner RE. Molecular Strategies in the Study and Repair of Palatal Defects. In: Stem Cell Biology and Tissue Engineering in Dental Sciences, (editors: A. Vishwakarma, P. Sharpe, S. Shi, X Wang, M Ramalingam), Elsevier Press (in press), (2013).
  • Neal RE, Chen J, Jagadapillai R, Jang H, Abomoelak B, Brock GN, Brock G, Greene RM, Pisano MM. Developmental cigarette smoke exposure: hippocampus proteome and metabolome profiles in low birth weight pups. Toxicology 317:40-49 (2014) PMID: 24486158
  • Greene RM, Pisano MP, Warner D.  Genetic and Epigenetic Perspectives of Orofacial Clefting. In: Comprehensive Cleft Care, 2nd edition. edit. Losee JE, Kirschner RE., McGraw-Hill (in press), (2014).
  • Warner DR, Pisano MM, Greene RM. PRDM16 in Development and Disease. Human Genetics & Embryology (in press).
  • Warner DR, Mukhopadhyay P, Brock G, Webb C, Pisano MM, Greene RM. MicroRNA expression profiling of the developing upper lip in mice. Development, Growth & Differentiation  (in press).

Partha Mukhopadhyay, Ph.D.

  • Warner DR, Mukhopadhyay P, Webb CL, Greene RM, Pisano MM. Chromatin immunoprecipitation-promoter microarray identification of genes regulated by PRDM16 in murine embryonic palate mesenchymal cells. Exp Biol Med (Maywood). 237(4):387-394 (2012). PubMed PMID:22522345.
  • Mukhopadhyay P, Brock G, Webb C, Pisano MM, Greene RM. Strain-specific modifier genes governing craniofacial phenotypes. Birth Defects Res A Clin Mol Teratol. 94:162-175, (2012). PMCID: PMC3302963.
  • Seelan RS, Warner DR, Mukhopadhyay P, Andres SA, Smolenkova IA, Wittliff JL, Michele Pisano M, Greene RM. Epigenetic analysis of laser capture microdissected fetal epithelia. Anal Biochem. 442(1):68-74, (2013). PubMed PMID: 23911529.
  • Seelan RS, Mukhopadhyay P, Pisano MM, Greene RM. Developmental epigenetics of the murine secondary palate. ILAR J. 53(3-4):240-252, (2012). PMCID:PMC4021823.
  • Seelan RS, Mukhopadhyay P, Warner DR, Webb CL, Pisano M, Greene RM. Epigenetic regulation of Sox4 during palate development. Epigenomics 5(2):131-146, (2013). PMCID: PMC3776497.
  • Seelan RS, Appana SN, Mukhopadhyay P, Warner DR, Brock GN, Pisano MM, Greene RM. Developmental profiles of the murine palatal methylome. Birth Defects Res A Clin Mol Teratol. 97(4):171-186 (2013). PMCID: PMC3776452.
  • Mukhopadhyay P, Rezzoug F, Kaikaus J, Greene RM, Pisano MM. Alcohol modulates expression of DNA methyltransferases and methyl CpG-/CpG domain-binding proteins in murine embryonic fibroblasts. Reprod Toxicol. 37:40-48 (2013). PubMed PMID: 23395981.
  • Brock GN, Mukhopadhyay P, Pihur V, Webb C, Greene RM, Pisano MM. MmPalateMiRNA, an R package compendium illustrating analysis of miRNA microarray data. Source Code Biol Med. 8(1):1, (2013). PMCID: PMC3654997.
  • Liu Y, Mukhopadhyay P, Pisano MM, Lu X, Huang L, Lu Q, Dean DC. Repression of Zeb1 and hypoxia cause sequential mesenchymal-to-epithelial transition and induction of aid, Oct4, and Dnmt1, leading to immortalization and multipotential  reprogramming of fibroblasts in spheres. Stem Cells. 31(7):1350-62. (2013). PubMed PMID: 23554223.
  • Warner DR, Mukhopadhyay P, Brock G, Webb CL, Pisano MM, Greene RM. 2014. MicroRNA expression profiling of the developing murine upper lip. Dev Growth Differ. May 22. doi: 10.1111/dgd.12140. [Epub ahead of print]. PubMed PMID:24849136.

 

M. Michele Pisano, Ph.D.

  • Mukhopadhyay P., C.L. Webb, M.M. Pisano, R.M. Greene.  2012.  Strain-specific modifier genes governing craniofacial phenotypes in ski-null mice. Birth Defects Res. A. 94:162-175.
  • Warner D.R., P. Mukhopadhyay, C.L. Webb, R.M. Greene, M.M. Pisano. 2012.  Chromatin immuno-precipitation-promoter microarray identification of genes regulated by PRDM16 in murine embryonic palate mesenchymal cells  Exper. Biol. Med. 237:387-394.  **Special feature on "EurekAlert!" science and medicine world news.
  • Canales L., J. Chen, E. Kelty, S. Musah, C. Webb, M.M. Pisano, R.E. Neal.  2012 . Developmental cigarette smoke exposure alters liver proteome profiles in low birth weight Pups. Toxicol. 300:1-11.
  • Rekha J, J. Chen, L. Canales, T.M. Birtles, M. M. Pisano, R.E. Neal. 2012.  Developmental cigarette smoke exposure alters kidney proteome Pprofiles. Toxicol.  299: 80-89.
  • Seelan R.S., P. Mukhopadhyay, M.M. Pisano, R.M. Greene.  2012.  Developmental epigenetics of the murine secondary palate. ILAR Journal, special issue “Epigenetics – From Mice to Men.” 53:240-252.
  • Amos-Kroohs RM, M.T, Williams, A.A. Brauna, D.L. Graham, C.L.  Webb, T.S. Birtles, R.M. Greene, C.V. Vorhees, M.M. Pisano. 2013.  Neurobehavioral phenotype of C57BL/6J mice prenatally and neonatally exposed to cigarette smoke. Neurobehav. Teratol Toxicol. 35:34-45.
  • Luijten M., A.V. Singh, C. Bastian, A Westerman, M.M. Pisano, J.L.A. Pennings, A. Verhoef,  M.L. Green, A.H.  Piersma,  A. de Vries, T.B. Knudsen.  2013.  Altered developmental programming of the mouse mammary gland in female offspring following perinatal dietary exposures. Plos ONE  February 2013 | Volume 8 | Issue 2 | e556.
  • Brock G.N., P. Mukhopadhyay, V. Pihur, C. Webb, R.M. Greene, M.M. Pisano. 2013.  MmPalateMiRNA, an R Package Compendium Illustrating Analysis of miRNA Microarray Data.  Source Code in Biol. and Med.  8:1-20.
  • Warner D.R., J.P. Wells, R.M. Greene, M.M. Pisano. 2013. Gene expression changes in the secondary palate and mandible of PRDM16-/- mice.  Cell Tissue Res. 351:445-452.
  • **Awarded the Society for Experimental Biology and Medicine’s “Best Clinical/Preclinical and Translational Paper of 2013”
  • Mukhopadhyay P., F. Rezzoug, J. Kaikaus, R.M. Greene and M.M. Pisano.  2013.  Alcohol modulates expression of DNA methyltransfereases and Methyl CpG/CpG domain-binding proteins in murine embryonic fibroblasts.  Repro. Toxicol. 37:40-48.
  • Seelan R.S., P. Mukhopadhyay, D.R. Warner, C.L. Webb, M.M. Pisano, R.M. Greene.  2013.  Epigenetic Regulation of Sox4 During Palate Development.  Epigenomics 5:131-146.
  • Seelan R.S., S.N. Appana, P. Mukhopadhyay, D.R. Warner, G.N. Brock, M.M. Pisano, R.M. Greene. 2013.  Developmental profiles of the murine palatal methylome.  Birth Defects Res. A  97:171-186.
  • Liu Y., P.  Mukhopadhyay, M.M. Pisano, X. Lu, L. Huang, Q. Lu, D.C. Dean.  2013.  Repression of Zeb1 and hypoxia cause sequential MET and induction of Aid, Oct4, and Dnmt1, leading to immortalization and multipotential reprogramming of fibroblasts in spheres.  Stem Cells  31:1350-1362.
  • Seelan R.S., I. Smolenkova, S. Andres, J.L. Wittliff,  P. Mukhopadhyay, D.R. Warner, M.M. Pisano, R.M. Greene. 2013. Epigenetic Analysis of Laser Capture Microdissected Fetal Epithelia. Analyt. Biochem. 443:68-74. 
  • Green M.L., M.M. Pisano, R.A. Prough, T.B. Knudsen.  2013.  Release of targeted p53 from the mitochondrion as an early signal during mitochondrial dysfunction.  Cell. Signal.  25:2383-2390.
  • Neal, R.E.,. J. Chen, Rekha J, H. Jang, G. N. Brock, B. Abomoelak, R.M. Greene, M. M. Pisano.  2014.  Developmental cigarette smoke exposure:  Hippocampus proteome and metabolome profiles in low birthweight pups.   Toxicology 317:40-49.
  • Warner D.R., P. Mukhopadhyay, G. Brock, C. Webb, M.M. Pisano, R.M. Greene.   2014.  MicroRNA expression profiling of the developing murine upper lip. Develop., Growth & Differentiation May 22. doi: 10.1111/dgd.12140. [Epub ahead of print]
  • Warner D.R., R.M. Greene, M.M. Pisano.  2014.  PRDM16 in Development and Disease. Human Genet. & Embryol. 4:121. doi: 10.4172/2161-0436.1000121
  • Jin J.-Z., D.R. Warner, Q. Lu Q, M.M. Pisano, R.M. Greene J.  Ding.  2014.  Deciphering TGFß3 function in medial edge epithelium specification and fusion during mouse secondary palate development. Submitted to:  Developmental Dynamics Aug 8. doi: 10.1002/dvdy.24177 [Epub ahead of print]
  • Greene, R.M. and M.M. Pisano. 2014  “Perspectives in orofacial cleft research III:  Molecular mechanisms” in Comprehsensive Cleft Care, 2nd Edition,  Edit. Lossee, JE and Kirschner, RE; McGraw-Hill Inc., New York.

 

Lisa Sandell, Ph.D.

  • Sandell, LL, in Neural crest cells: evolution, development and disease, P. A. Trainor, Ed. Academic Press, London, (2014),  pp. 167-188.doi:10.1038/sj.bdj.2014.423
  • Sandell LL, Butler Tjaden NE, Barlow AJ, Trainor PA. Cochleovestibular nerve development is integrated with migratory neural crest cells. Dev Biol.  385(2): 200-10. (2014).  PMID:24252775
  • Cunningham Thomas J, Zhao X, Sandell Lisa L, Evans Sylvia M, Trainor Paul A, Duester G.  Antagonism between retinoic acid and fibroblast growth factor signaling during limb development. Cell Reports.  3(5): 1503-11. (2013). PMCID: PMC3745640.
  • Sandell LL, Lynn ML, Inman KE, McDowell W, Trainor PA. Rdh10 oxidation of vitamin a is a critical control step in synthesis of retinoic acid during mouse embryogenesis. PLoS ONE.  7(2): e30698. (2012). PMCID: PMC3271098.
  • Sandell LL, Kurosaka H, Trainor PA.  Whole mount nuclear fluorescent imaging: Convenient documentation of embryo morphology. Genesis.  50(11): 844-50. doi: 10.1002/dvg.22344. (2012).  PMCID: PMC3504639.

 

Seelan, Ratnam, Ph.D.

  • Lakshmanan J, Seelan RS, Vadnal RE, Janckila AJ, Casanova MF, Parthasarathy LK, Parthasarathy RN. Proteomic analysis of rat prefrontal cortex after chronic lithium treatment. J. Proteomics Bioinform. 5: 140-146 (2012).
  • Seelan RS, Mukhopadhyay P, Pisano MM, Greene RM. Developmental epigenetics of the murine secondary palate. ILAR Journal 53: 240-252 (2012). PMID: 23744964.
  • Seelan RS, Mukhopadhyay P, Warner DR, Webb CL, Pisano MM, Greene RM. The epigenetics of Sox4 regulation in murine palatogenesis. Epigenomics 5: 131-146 (2013). PMID: 23566091
  • Parthasarathy RN, Lakshmanan J, Thangavel M, Seelan RS, Stagner JI, Janckila AJ, Vadnal RE, Casanova MF, Parthasarathy LK.Rat brain myo-inositol 3-phosphate synthase is a phosphoprotein. Mol. Cell. Biochem. 378: 83-89 (2013). PMID: 23504145.
  • Seelan RS, Appana SN, Mukhopadhyay P, Warner DR, Brock GN, Pisano MM, Greene RM. Developmental profiles of the murine palatal methylome. Birth Defects Res A Clin Mol Teratol. 97: 171-186 (2013). PMID: 23554260
  • Seelan RS, Warner DR, Mukhopadhyay P, Andres SA, Smolenkova IA, Wittliff JL, Pisano MM, Greene RM. Epigenetic analysis of laser capture microdissected fetal epithelia. Anal. Biochem. 442: 68-74 (2013). PMID: 23911529
  • Thangavel M, Seelan RS, Lakshmanan J, Vadnal RE, Stagner JI, Parthasarathy LK, Casanova MF, Parthasarathy RN. Proteomic analysis of rat prefrontal cortex after chronic valproate treatment. J. Neurosci. Res. 92: 927-936 (2014). PMID: 24692022

 

Dennis Warner, Ph.D.

  • D.R. Warner, P. Mukhopadhyay, C.L. Webb, R.M. Greene, M.M. Pisano (2012) Experimental Biology and Medicine 237:387-394.  “Chromatin immunoprecipitation-promoter microarray identification of genes regulated by PRDM16 in murine embryonic palate mesenchymal cells”
  • D.R. Warner, J.P. Wells, R.M. Greene, M.M. Pisano (2012) Cell Tissue Research 351:445-452.  “Gene expression changes in the secondary palate and mandible of Prdm16-/- mice”. 
  • R.S. Seelan, S.N. Appana, P. Mukhopadhyay, D.R. Warner, G.N. Brock, M.M. Pisano, R.M. Greene. (2013) Birth Def. Res. A Clin. Mol. Teratol. 97:171-186.  “Developmental profiles of the murine palatal methylome”. 
  • R.S. Seelan, P. Mukhopadhyay, D.R. Warner, C.L. Webb, M.M. Pisano, R.M. Greene (2013) Epigenomics 5:131-146. “Epigenetic regulation of Sox4 during palate development”.
  • Z. Gu, A.Y. Chhabra, P.Alard, D.R. Warner, M.M. Kosiewicz (2013) Immunobiology 218:1200-1206. “FcgRI is required for TGFb2-treated macrophage-induced tolerance”.
  • R.S. Seelan, D.R. Warner, P. Mukhopadhyay, S.A. Andres, I.A. Smolenkova, J.L. Wittliff, M.M. Pisano, R.M. Greene (2013) Analytical Biochemistry 442:68-74. “Epigenetic analysis of laser capture microdissected fetal epithelia”.
  • D.R. Warner, P. Mukhopadhyay,  G. Brock, C.L. Webb, M.M. Pisano, R.M. Greene (2014) Development Growth and Differentiation. “MicroRNA expression profiling of the developing murine upper lip”.
  • D.R. Warner, R.M. Greene, M.M. Pisano (2014) Human Genetics and  Embryology. 4:1-4. “PRDM16 in development and disease”. (Review Article). 
  • J.-Z.  Jin, D.R.  Warner, Q.  Lu, M. M.  Pisano, R.M.  Greene, and J. Ding (2014) Developmental Biology. “Deciphering TGF-b3 function in medial edge epithelium specification and fusion during mouse secondary palate development”.  In Press.

 

Research Funding

Jixiang Ding
Role: Principal Investigator
Title:  Mechanism of mammalian embryonic heart formation
Funding Agency: KSEF
Total Direct cost requested: $72,000
Period: 07/01/2010-06/30/2012

Role: Principal Investigator
Title: Regulation of Nodal signaling in holoprosencephaly
Funding Agency: National Institutes of Health
Type: R01
Total Direct Costs: $1,125,000
Period: In no cost extension

Role: Principal Investigator
Title: Regulation of Nodal signaling in holoprosencephaly
Funding Agency: National Institutes of Health
Type: K02 career development award
Total Direct Costs: $482,958
Period: ended in June 2010.

Robert Greene
Role: Principal Investigator
Title: TRANSCRIPTIONAL COACTIVATORS AND PREGNANCY OUTCOMES
Funding Agency: National Institutes of Health
Type: R01–HD053509
Total Direct Costs: $1,321,365
Period: 2008-2013

Role: Principal Investigator
Title: NUTRITIONAL EPIGENETICS AND OROFACIAL DEVELOPMENT
Funding Agency: National Institutes of Health
Type: R01–DE018215
Total Direct Costs: $900,000
Period: 2008-2012

Role: Principal Investigator
Title: MOLECULAR DETERMINANTS OF DEVELOPMENTAL DEFECTS
NCRR Center for Biomedical Research Excellence
Funding Agency: NIH, NCRR
Type: P20-RR/DE17702
Total Direct Costs: $12,072,292
Period: 2002-2013

Role: Principal Investigator
Title: Birth Defects Center Postdoctoral Fellowship
Funding Agency: Kosair Charities
Total Direct Costs: $25,000
Period: 2010-2011

ZI-JIAN LAN
Role:  COBRE supported junior investigator
Title: Molecular studies of male and female reproduction
Funding Agency: National Institutes of Health/National Center for Research Resources
Grant Type: Center of Biological Research Excellence (COBRE)
Annual Direct Costs Funded:  $157,500

PARTHA MUKHOPADHYAY

Role: Co-Investigator [Co-PIs: Eric Rouchka and Ted Kalbfleisch]
Title: Extension of Informatics Infrastructure to Support Translational and Basic Research
Funding Agency: Department of Energy
Direct Costs Funded: $1,194,300

M. MICHELE PISANO

P.I.:    M. Michele Pisano
P.I.   Rachel Neal
Title:     Developmental Cigarette Smoke Exposure: Biomarkers of Neurotoxicity   
Funding Agency:  NIH R21DA027466
Total Direct Costs Funded:  $400,000
Project Period: 2009 - 2012

P.I.:    M. Michele Pisano
P.I.   Thomas B. Knudsen
Title:  Response Signatures of Alcohol Related Birth Defects
Funding Agency:  NIH R01 AA13205
Total Direct Costs Funded:  $221,582.

P.I.:    M. Michele Pisano
P.I.:  Thomas B. Knudsen
Title: Perinatal Programming of Breast Cancer:  Fat and Estrogens
Funding Agency:  NIH R01 ES013821
Total Direct Costs Funded:  $104,000.

Subproject Director:    M. Michele Pisano
P.I.:  Robert M Greene
Title:    Molecular Determinants of Developmental Defects
Subroject:  Pre- and Postnatal Tobacco Smoke Exposure:  Effects on Neurocognitive Development
Funding Agency:  NIH P20 RR/DE017702
Subproject Direct Costs Funded: $540,000

P.I.:  M. Michele Pisano
Title:  “Developmental Neurotoxicity of Prenatal Environmental Tobacco Smoke Exposure”
Funding Agency: University of Louisville Center for Environmental Genomics and
Integrative Biology,  Kenneth Ramos, Director.
Direct Costs Funded: $30,000.       

Co-I.: M. Michele Pisano
P.I.:  Robert M. Greene
Title: “Nutritional Epigenetics and Orofacial Development”
Funding Agency: NIH R01 DE018215
Total Direct Costs Funded:  $900,000.

Co-I.: M. Michele Pisano
P.I.:  Robert M. Greene
Title:  “Transcriptional Coactivators and Pregnancy Outcomes”
Funding Agency: NIH R01 HD053509
Total Direct Costs Funded:  $1,321,365.

Ratnam Seelan
Role: Sub-project Director
P.I.:  Robert M Greene
Title: Regulation of Neural Crest Cell Migration by SDF1-CXCR4 Signaling.
Funding Agency:  NIH P20 RR/DE017702
Direct Costs Funded: $450,000 (2008-2011).